Identifikasi Tomato infectious chlorosis virus dan Tomato chlorosis virus melalui Reverse Transcription Polymerase Chain Reaction dan Analisis Sikuen Nukleotida
<p>Tomato chlorosis disease was found more frequent on production area in West Java recently. Observation in the field showed typical symptoms of <em>Tomato infectious chlorosis virus </em>(TICV) and <em>Tomato chlorosis virus </em>(ToCV). The objective of this research...
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Perhimpunan Fitopatologi Indonesia
2014-08-01
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Online Access: | http://journal.ipb.ac.id/index.php/jfiti/article/view/8072 |
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doaj-4b405602415444ad82f7d7cbaf4d21a02020-11-24T23:16:13ZindPerhimpunan Fitopatologi IndonesiaJurnal Fitopatologi Indonesia0215-79502339-24792014-08-019410.14692/jfi.9.4.1077167Identifikasi Tomato infectious chlorosis virus dan Tomato chlorosis virus melalui Reverse Transcription Polymerase Chain Reaction dan Analisis Sikuen NukleotidaSari Nurulita0Gede Suastika1Institut Pertanian BogorInstitut Pertanian Bogor<p>Tomato chlorosis disease was found more frequent on production area in West Java recently. Observation in the field showed typical symptoms of <em>Tomato infectious chlorosis virus </em>(TICV) and <em>Tomato chlorosis virus </em>(ToCV). The objective of this research is to identify TICV and ToCV associated with chlorosis disease on tomato plants in West Java (Cipanas, Lembang and Garut) using <em>reverse transcription polymerase chain reaction </em>(RT-PCR) method and nucleotide sequence analysis. DNA target of 417 bp and 360 bp was successfully amplified only from Cipanas sample using specific primers, TICV-CF/TICV-CR and ToCV-CF/ToCV-CR, respectively. Sequence analysis confirmed that chlorosis disease in Cipanas was associated with TICV and ToCV infection. Nucleotide sequence and phylogenetic analysis showed that TICV from Cipanas has high homology to and belongs to the same group with TICV from Japan and Spain; whereas ToCV from Cipanas has high homology to and belongs to the same group with ToCV from America.</p>http://journal.ipb.ac.id/index.php/jfiti/article/view/8072Bemisia tabaci, crinivirus, phylogenetic, Trialeuroides vaporariorum |
collection |
DOAJ |
language |
Indonesian |
format |
Article |
sources |
DOAJ |
author |
Sari Nurulita Gede Suastika |
spellingShingle |
Sari Nurulita Gede Suastika Identifikasi Tomato infectious chlorosis virus dan Tomato chlorosis virus melalui Reverse Transcription Polymerase Chain Reaction dan Analisis Sikuen Nukleotida Jurnal Fitopatologi Indonesia Bemisia tabaci, crinivirus, phylogenetic, Trialeuroides vaporariorum |
author_facet |
Sari Nurulita Gede Suastika |
author_sort |
Sari Nurulita |
title |
Identifikasi Tomato infectious chlorosis virus dan Tomato chlorosis virus melalui Reverse Transcription Polymerase Chain Reaction dan Analisis Sikuen Nukleotida |
title_short |
Identifikasi Tomato infectious chlorosis virus dan Tomato chlorosis virus melalui Reverse Transcription Polymerase Chain Reaction dan Analisis Sikuen Nukleotida |
title_full |
Identifikasi Tomato infectious chlorosis virus dan Tomato chlorosis virus melalui Reverse Transcription Polymerase Chain Reaction dan Analisis Sikuen Nukleotida |
title_fullStr |
Identifikasi Tomato infectious chlorosis virus dan Tomato chlorosis virus melalui Reverse Transcription Polymerase Chain Reaction dan Analisis Sikuen Nukleotida |
title_full_unstemmed |
Identifikasi Tomato infectious chlorosis virus dan Tomato chlorosis virus melalui Reverse Transcription Polymerase Chain Reaction dan Analisis Sikuen Nukleotida |
title_sort |
identifikasi tomato infectious chlorosis virus dan tomato chlorosis virus melalui reverse transcription polymerase chain reaction dan analisis sikuen nukleotida |
publisher |
Perhimpunan Fitopatologi Indonesia |
series |
Jurnal Fitopatologi Indonesia |
issn |
0215-7950 2339-2479 |
publishDate |
2014-08-01 |
description |
<p>Tomato chlorosis disease was found more frequent on production area in West Java recently. Observation in the field showed typical symptoms of <em>Tomato infectious chlorosis virus </em>(TICV) and <em>Tomato chlorosis virus </em>(ToCV). The objective of this research is to identify TICV and ToCV associated with chlorosis disease on tomato plants in West Java (Cipanas, Lembang and Garut) using <em>reverse transcription polymerase chain reaction </em>(RT-PCR) method and nucleotide sequence analysis. DNA target of 417 bp and 360 bp was successfully amplified only from Cipanas sample using specific primers, TICV-CF/TICV-CR and ToCV-CF/ToCV-CR, respectively. Sequence analysis confirmed that chlorosis disease in Cipanas was associated with TICV and ToCV infection. Nucleotide sequence and phylogenetic analysis showed that TICV from Cipanas has high homology to and belongs to the same group with TICV from Japan and Spain; whereas ToCV from Cipanas has high homology to and belongs to the same group with ToCV from America.</p> |
topic |
Bemisia tabaci, crinivirus, phylogenetic, Trialeuroides vaporariorum |
url |
http://journal.ipb.ac.id/index.php/jfiti/article/view/8072 |
work_keys_str_mv |
AT sarinurulita identifikasitomatoinfectiouschlorosisvirusdantomatochlorosisvirusmelaluireversetranscriptionpolymerasechainreactiondananalisissikuennukleotida AT gedesuastika identifikasitomatoinfectiouschlorosisvirusdantomatochlorosisvirusmelaluireversetranscriptionpolymerasechainreactiondananalisissikuennukleotida |
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1725588220997009408 |