Tissue alkaline phosphatase activity and expression in an experimental infant swine model of cardiopulmonary bypass with deep hypothermic circulatory arrest
Abstract Background Infant cardiac surgery with cardiopulmonary bypass results in decreased circulating alkaline phosphatase that is associated with poor postoperative outcomes. Bovine intestinal alkaline phosphatase infusion represents a novel therapy for post-cardiac surgery organ injury. However,...
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doaj-4b4a840a301c49a4b4b1d5cb07f9b0012020-11-25T03:19:51ZengBMCJournal of Inflammation1476-92552020-08-0117111310.1186/s12950-020-00256-2Tissue alkaline phosphatase activity and expression in an experimental infant swine model of cardiopulmonary bypass with deep hypothermic circulatory arrestLudmila Khailova0Justin Robison1James Jaggers2Richard Ing3Scott Lawson4Amy Treece5Danielle Soranno6Suzanne Osorio Lujan7Jesse A. Davidson8Department of Pediatrics, University of ColoradoDepartment of Pediatrics, University of ColoradoDepartment of Surgery, University of ColoradoDepartment of Anesthesiology, University of ColoradoChildren’s Hospital Colorado, Heart InstituteDepartment of Pathology, University of ColoradoDepartment of Pediatrics, University of ColoradoDepartment of Pediatrics, University of ColoradoDepartment of Pediatrics, University of ColoradoAbstract Background Infant cardiac surgery with cardiopulmonary bypass results in decreased circulating alkaline phosphatase that is associated with poor postoperative outcomes. Bovine intestinal alkaline phosphatase infusion represents a novel therapy for post-cardiac surgery organ injury. However, the effects of cardiopulmonary bypass and bovine-intestinal alkaline phosphatase infusion on tissue-level alkaline phosphatase activity/expression are unknown. Methods Infant pigs (n = 20) underwent cardiopulmonary bypass with deep hypothermic circulatory arrest followed by four hours of intensive care. Seven control animals underwent mechanical ventilation only. Cardiopulmonary bypass/deep hypothermic circulatory arrest animals were given escalating doses of bovine intestinal alkaline phosphatase infusion (0-25 U/kg/hr.; n = 5/dose). Kidney, liver, ileum, jejunum, colon, heart and lung were collected for measurement of tissue alkaline phosphatase activity and mRNA. Results Tissue alkaline phosphatase activity varied significantly across organs with the highest levels found in the kidney and small intestine. Cardiopulmonary bypass with deep hypothermic circulatory arrest resulted in decreased kidney alkaline phosphatase activity and increased lung alkaline phosphatase activity, with no significant changes in the other organs. Alkaline phosphatase mRNA expression was increased in both the lung and the ileum. The highest dose of bovine intestinal alkaline phosphatase resulted in increased kidney and liver tissue alkaline phosphatase activity. Conclusions Changes in alkaline phosphatase activity after cardiopulmonary bypass with deep hypothermic circulatory arrest and bovine intestinal alkaline phosphatase delivery are tissue specific. Kidneys, lung, and ileal alkaline phosphatase appear most affected by cardiopulmonary bypass with deep hypothermic circulatory arrest and further research is warranted to determine the mechanism and biologic importance of these changes.http://link.springer.com/article/10.1186/s12950-020-00256-2Cardiac surgeryOrgan injuryInflammationEndotoxinAcute kidney injuryAcute lung injury |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ludmila Khailova Justin Robison James Jaggers Richard Ing Scott Lawson Amy Treece Danielle Soranno Suzanne Osorio Lujan Jesse A. Davidson |
spellingShingle |
Ludmila Khailova Justin Robison James Jaggers Richard Ing Scott Lawson Amy Treece Danielle Soranno Suzanne Osorio Lujan Jesse A. Davidson Tissue alkaline phosphatase activity and expression in an experimental infant swine model of cardiopulmonary bypass with deep hypothermic circulatory arrest Journal of Inflammation Cardiac surgery Organ injury Inflammation Endotoxin Acute kidney injury Acute lung injury |
author_facet |
Ludmila Khailova Justin Robison James Jaggers Richard Ing Scott Lawson Amy Treece Danielle Soranno Suzanne Osorio Lujan Jesse A. Davidson |
author_sort |
Ludmila Khailova |
title |
Tissue alkaline phosphatase activity and expression in an experimental infant swine model of cardiopulmonary bypass with deep hypothermic circulatory arrest |
title_short |
Tissue alkaline phosphatase activity and expression in an experimental infant swine model of cardiopulmonary bypass with deep hypothermic circulatory arrest |
title_full |
Tissue alkaline phosphatase activity and expression in an experimental infant swine model of cardiopulmonary bypass with deep hypothermic circulatory arrest |
title_fullStr |
Tissue alkaline phosphatase activity and expression in an experimental infant swine model of cardiopulmonary bypass with deep hypothermic circulatory arrest |
title_full_unstemmed |
Tissue alkaline phosphatase activity and expression in an experimental infant swine model of cardiopulmonary bypass with deep hypothermic circulatory arrest |
title_sort |
tissue alkaline phosphatase activity and expression in an experimental infant swine model of cardiopulmonary bypass with deep hypothermic circulatory arrest |
publisher |
BMC |
series |
Journal of Inflammation |
issn |
1476-9255 |
publishDate |
2020-08-01 |
description |
Abstract Background Infant cardiac surgery with cardiopulmonary bypass results in decreased circulating alkaline phosphatase that is associated with poor postoperative outcomes. Bovine intestinal alkaline phosphatase infusion represents a novel therapy for post-cardiac surgery organ injury. However, the effects of cardiopulmonary bypass and bovine-intestinal alkaline phosphatase infusion on tissue-level alkaline phosphatase activity/expression are unknown. Methods Infant pigs (n = 20) underwent cardiopulmonary bypass with deep hypothermic circulatory arrest followed by four hours of intensive care. Seven control animals underwent mechanical ventilation only. Cardiopulmonary bypass/deep hypothermic circulatory arrest animals were given escalating doses of bovine intestinal alkaline phosphatase infusion (0-25 U/kg/hr.; n = 5/dose). Kidney, liver, ileum, jejunum, colon, heart and lung were collected for measurement of tissue alkaline phosphatase activity and mRNA. Results Tissue alkaline phosphatase activity varied significantly across organs with the highest levels found in the kidney and small intestine. Cardiopulmonary bypass with deep hypothermic circulatory arrest resulted in decreased kidney alkaline phosphatase activity and increased lung alkaline phosphatase activity, with no significant changes in the other organs. Alkaline phosphatase mRNA expression was increased in both the lung and the ileum. The highest dose of bovine intestinal alkaline phosphatase resulted in increased kidney and liver tissue alkaline phosphatase activity. Conclusions Changes in alkaline phosphatase activity after cardiopulmonary bypass with deep hypothermic circulatory arrest and bovine intestinal alkaline phosphatase delivery are tissue specific. Kidneys, lung, and ileal alkaline phosphatase appear most affected by cardiopulmonary bypass with deep hypothermic circulatory arrest and further research is warranted to determine the mechanism and biologic importance of these changes. |
topic |
Cardiac surgery Organ injury Inflammation Endotoxin Acute kidney injury Acute lung injury |
url |
http://link.springer.com/article/10.1186/s12950-020-00256-2 |
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