Evaluation of lambda-cyhalothrin oxidative stress and gonad histoarchitecture toxicity potency in Clarias gariepinus

• Main Authors: Samuel U. Ezenwosu, Emmanuel I. Nnamonu, Gregory Ejikeme Odo, Bright C. Ikele, Ogonna C. Ani
• Format: Article
• Language: English
• Published: SpringerOpen 2021-01-01
• Series: Journal of Basic and Applied Zoology
• Subjects: Lambda-cyhalothrin; Clarias gariepinus; Oxidative stress biomarkers; Gonad-histology
• Online Access: https://doi.org/10.1186/s41936-020-00201-y

Abstract Background Extensive and indiscriminate use of pesticides gradually destroys the environment (ecosystem), poses serious threats to human health, animal life (especially aquatic), plant forms, soil, water, and also lead to emergence of resilient species of life forms that are becoming resistant to pesticides. The present study focused on evaluating lambda-cyhalothrin oxidative stress and gonad histoarchitecture toxicity potency in Clarias gariepinus. Results A total of 120 C. gariepinus 16 to 40 cm SL and 200 to 250 g bodyweights (assigned into treatments 0.00 (control), 2.5 × 10−4 μg/L, 5.0 × 10−4 μg/L, and 6.25×10−4 μg/L (A-D) lambda-cyhalothrin (LCT), each treatment consisted of 30 fishes, replicated three times, 10 fishes per replicate) were used for this study. On day 7, catalase activity (CAT) and glutathione peroxidase (GPx) significantly increased (p < 0.05) in all treatments compared with control. Day 14, superoxide dismutase (SOD) and GPx significantly increased (p < 0.05). All parameters significantly increased (p < 0.05) on days 21 and 28 except SOD (day 21). All parameters increased significantly on day 28 across the row in all treatments. The significant increase (p < 0.05) in SOD, (malondialdehyde) MDA, GPx, and glutathione reductase (GR) levels returned to normal after 7 days of depuration but CAT level did not return to normal. The testes photomicrographs showed necrotic conditions in the spermatogenic cells with nuclear pyknosis and cytoplasmic swelling while that of the ovary displayed vacuolations, flabby oocytes, and degenerated ovaries changes. Conclusion Lambda-cyhalothrin is toxic to C. gariepinus. The inability of significant increase in CAT to return to normal after 7 days of depuration further confirms our report.