Quantifying the area-at-risk of myocardial infarction in-vivo using arterial spin labeling cardiac magnetic resonance

Abstract T2-weighted cardiovascular magnetic resonance (T2-CMR) of myocardial edema can quantify the area-at-risk (AAR) following acute myocardial infarction (AMI), and has been used to assess myocardial salvage by new cardioprotective therapies. However, some of these therapies may reduce edema, le...

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Main Authors: Rachel K. Dongworth, Adrienne E. Campbell-Washburn, Hector A. Cabrera-Fuentes, Heerajnarain Bulluck, Thomas Roberts, Anthony N. Price, Sauri Hernández-Reséndiz, Roger J. Ordidge, David L. Thomas, Derek M. Yellon, Mark F. Lythgoe, Derek J. Hausenloy
Format: Article
Language:English
Published: Nature Publishing Group 2017-05-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-02544-z
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spelling doaj-4b5a825347db4811b2bd0474faeac5142020-12-08T00:15:17ZengNature Publishing GroupScientific Reports2045-23222017-05-017111210.1038/s41598-017-02544-zQuantifying the area-at-risk of myocardial infarction in-vivo using arterial spin labeling cardiac magnetic resonanceRachel K. Dongworth0Adrienne E. Campbell-Washburn1Hector A. Cabrera-Fuentes2Heerajnarain Bulluck3Thomas Roberts4Anthony N. Price5Sauri Hernández-Reséndiz6Roger J. Ordidge7David L. Thomas8Derek M. Yellon9Mark F. Lythgoe10Derek J. Hausenloy11The Hatter Cardiovascular Institute, University College LondonCentre for Advanced Biomedical Imaging, University College LondonCardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical SchoolThe Hatter Cardiovascular Institute, University College LondonCentre for Advanced Biomedical Imaging, University College LondonDivision of Imaging Sciences and Biomedical Engineering, King’s College London, King’s Health Partners, St. Thomas’ HospitalCardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical SchoolCentre for Neuroscience, University of MelbourneDepartment of Brain Repair and Rehabilitation, UCL Institute of Neurology, University College LondonThe Hatter Cardiovascular Institute, University College LondonCentre for Advanced Biomedical Imaging, University College LondonThe Hatter Cardiovascular Institute, University College LondonAbstract T2-weighted cardiovascular magnetic resonance (T2-CMR) of myocardial edema can quantify the area-at-risk (AAR) following acute myocardial infarction (AMI), and has been used to assess myocardial salvage by new cardioprotective therapies. However, some of these therapies may reduce edema, leading to an underestimation of the AAR by T2-CMR. Here, we investigated arterial spin labeling (ASL) perfusion CMR as a novel approach to quantify the AAR following AMI. Adult B6sv129-mice were subjected to in vivo left coronary artery ligation for 30 minutes followed by 72 hours reperfusion. T2-mapping was used to quantify the edema-based AAR (% of left ventricle) following ischemic preconditioning (IPC) or cyclosporin-A (CsA) treatment. In control animals, the AAR by T2-mapping corresponded to that delineated by histology. As expected, both IPC and CsA reduced MI size. However, IPC, but not CsA, also reduced myocardial edema leading to an underestimation of the AAR by T2-mapping. In contrast, regions of reduced myocardial perfusion delineated by cardiac ASL were able to delineate the AAR when compared to both T2-mapping and histology in control animals, and were not affected by either IPC or CsA. Therefore, ASL perfusion CMR may be an alternative method for quantifying the AAR following AMI, which unlike T2-mapping, is not affected by IPC.https://doi.org/10.1038/s41598-017-02544-z
collection DOAJ
language English
format Article
sources DOAJ
author Rachel K. Dongworth
Adrienne E. Campbell-Washburn
Hector A. Cabrera-Fuentes
Heerajnarain Bulluck
Thomas Roberts
Anthony N. Price
Sauri Hernández-Reséndiz
Roger J. Ordidge
David L. Thomas
Derek M. Yellon
Mark F. Lythgoe
Derek J. Hausenloy
spellingShingle Rachel K. Dongworth
Adrienne E. Campbell-Washburn
Hector A. Cabrera-Fuentes
Heerajnarain Bulluck
Thomas Roberts
Anthony N. Price
Sauri Hernández-Reséndiz
Roger J. Ordidge
David L. Thomas
Derek M. Yellon
Mark F. Lythgoe
Derek J. Hausenloy
Quantifying the area-at-risk of myocardial infarction in-vivo using arterial spin labeling cardiac magnetic resonance
Scientific Reports
author_facet Rachel K. Dongworth
Adrienne E. Campbell-Washburn
Hector A. Cabrera-Fuentes
Heerajnarain Bulluck
Thomas Roberts
Anthony N. Price
Sauri Hernández-Reséndiz
Roger J. Ordidge
David L. Thomas
Derek M. Yellon
Mark F. Lythgoe
Derek J. Hausenloy
author_sort Rachel K. Dongworth
title Quantifying the area-at-risk of myocardial infarction in-vivo using arterial spin labeling cardiac magnetic resonance
title_short Quantifying the area-at-risk of myocardial infarction in-vivo using arterial spin labeling cardiac magnetic resonance
title_full Quantifying the area-at-risk of myocardial infarction in-vivo using arterial spin labeling cardiac magnetic resonance
title_fullStr Quantifying the area-at-risk of myocardial infarction in-vivo using arterial spin labeling cardiac magnetic resonance
title_full_unstemmed Quantifying the area-at-risk of myocardial infarction in-vivo using arterial spin labeling cardiac magnetic resonance
title_sort quantifying the area-at-risk of myocardial infarction in-vivo using arterial spin labeling cardiac magnetic resonance
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2017-05-01
description Abstract T2-weighted cardiovascular magnetic resonance (T2-CMR) of myocardial edema can quantify the area-at-risk (AAR) following acute myocardial infarction (AMI), and has been used to assess myocardial salvage by new cardioprotective therapies. However, some of these therapies may reduce edema, leading to an underestimation of the AAR by T2-CMR. Here, we investigated arterial spin labeling (ASL) perfusion CMR as a novel approach to quantify the AAR following AMI. Adult B6sv129-mice were subjected to in vivo left coronary artery ligation for 30 minutes followed by 72 hours reperfusion. T2-mapping was used to quantify the edema-based AAR (% of left ventricle) following ischemic preconditioning (IPC) or cyclosporin-A (CsA) treatment. In control animals, the AAR by T2-mapping corresponded to that delineated by histology. As expected, both IPC and CsA reduced MI size. However, IPC, but not CsA, also reduced myocardial edema leading to an underestimation of the AAR by T2-mapping. In contrast, regions of reduced myocardial perfusion delineated by cardiac ASL were able to delineate the AAR when compared to both T2-mapping and histology in control animals, and were not affected by either IPC or CsA. Therefore, ASL perfusion CMR may be an alternative method for quantifying the AAR following AMI, which unlike T2-mapping, is not affected by IPC.
url https://doi.org/10.1038/s41598-017-02544-z
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