The miR-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo development
Previously, we reported that Sebox is a new maternal effect gene (MEG) that is required for early embryo development beyond the two-cell (2C) stage because this gene orchestrates the expression of important genes for zygotic genome activation (ZGA). However, regulators of Sebox expression remain unk...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
The Royal Society
2016-01-01
|
Series: | Open Biology |
Subjects: | |
Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.160181 |
id |
doaj-4b67966ca1fe4a58a70141a80e2a7c29 |
---|---|
record_format |
Article |
spelling |
doaj-4b67966ca1fe4a58a70141a80e2a7c292020-11-25T03:28:26ZengThe Royal SocietyOpen Biology2046-24412016-01-0161110.1098/rsob.160181160181The miR-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo developmentKyeoung-Hwa KimYou-Mi SeoEun-Young KimSu-Yeon LeeJini KwonJung-Jae KoKyung-Ah LeePreviously, we reported that Sebox is a new maternal effect gene (MEG) that is required for early embryo development beyond the two-cell (2C) stage because this gene orchestrates the expression of important genes for zygotic genome activation (ZGA). However, regulators of Sebox expression remain unknown. Therefore, the objectives of the present study were to use bioinformatics tools to identify such regulatory microRNAs (miRNAs) and to determine the effects of the identified miRNAs on Sebox expression. Using computational algorithms, we identified a motif within the 3′UTR of Sebox mRNA that is specific to the seed region of the miR-125 family, which includes miR-125a-5p, miR-125b-5p and miR-351-5p. During our search for miRNAs, we found that the Lin28a 3′UTR also contains the same binding motif for the seed region of the miR-125 family. In addition, we confirmed that Lin28a also plays a role as a MEG and affects ZGA at the 2C stage, without affecting oocyte maturation or fertilization. Thus, we provide the first report indicating that the miR-125 family plays a crucial role in regulating MEGs related to the 2C block and in regulating ZGA through methods such as affecting Sebox and Lin28a in oocytes and embryos.https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.160181seboxlin28amir-125 familytranslational regulationbioinformatics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kyeoung-Hwa Kim You-Mi Seo Eun-Young Kim Su-Yeon Lee Jini Kwon Jung-Jae Ko Kyung-Ah Lee |
spellingShingle |
Kyeoung-Hwa Kim You-Mi Seo Eun-Young Kim Su-Yeon Lee Jini Kwon Jung-Jae Ko Kyung-Ah Lee The miR-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo development Open Biology sebox lin28a mir-125 family translational regulation bioinformatics |
author_facet |
Kyeoung-Hwa Kim You-Mi Seo Eun-Young Kim Su-Yeon Lee Jini Kwon Jung-Jae Ko Kyung-Ah Lee |
author_sort |
Kyeoung-Hwa Kim |
title |
The miR-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo development |
title_short |
The miR-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo development |
title_full |
The miR-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo development |
title_fullStr |
The miR-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo development |
title_full_unstemmed |
The miR-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo development |
title_sort |
mir-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo development |
publisher |
The Royal Society |
series |
Open Biology |
issn |
2046-2441 |
publishDate |
2016-01-01 |
description |
Previously, we reported that Sebox is a new maternal effect gene (MEG) that is required for early embryo development beyond the two-cell (2C) stage because this gene orchestrates the expression of important genes for zygotic genome activation (ZGA). However, regulators of Sebox expression remain unknown. Therefore, the objectives of the present study were to use bioinformatics tools to identify such regulatory microRNAs (miRNAs) and to determine the effects of the identified miRNAs on Sebox expression. Using computational algorithms, we identified a motif within the 3′UTR of Sebox mRNA that is specific to the seed region of the miR-125 family, which includes miR-125a-5p, miR-125b-5p and miR-351-5p. During our search for miRNAs, we found that the Lin28a 3′UTR also contains the same binding motif for the seed region of the miR-125 family. In addition, we confirmed that Lin28a also plays a role as a MEG and affects ZGA at the 2C stage, without affecting oocyte maturation or fertilization. Thus, we provide the first report indicating that the miR-125 family plays a crucial role in regulating MEGs related to the 2C block and in regulating ZGA through methods such as affecting Sebox and Lin28a in oocytes and embryos. |
topic |
sebox lin28a mir-125 family translational regulation bioinformatics |
url |
https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.160181 |
work_keys_str_mv |
AT kyeounghwakim themir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment AT youmiseo themir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment AT eunyoungkim themir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment AT suyeonlee themir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment AT jinikwon themir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment AT jungjaeko themir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment AT kyungahlee themir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment AT kyeounghwakim mir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment AT youmiseo mir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment AT eunyoungkim mir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment AT suyeonlee mir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment AT jinikwon mir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment AT jungjaeko mir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment AT kyungahlee mir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment |
_version_ |
1724584187833352192 |