Vinasse fertirrigation alters soil resistome dynamics: an analysis based on metagenomic profiles
Abstract Every year around 300 Gl of vinasse, a by-product of ethanol distillation in sugarcane mills, are flushed into more than 9 Mha of sugarcane cropland in Brazil. This practice links fermentation waste management to fertilization for plant biomass production, and it is known as fertirrigation....
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doaj-4b7471573dd04b19af04cd355f95ef462020-11-25T00:24:08ZengBMCBioData Mining1756-03812017-05-011011710.1186/s13040-017-0138-4Vinasse fertirrigation alters soil resistome dynamics: an analysis based on metagenomic profilesLucas P. P. Braga0Rafael F. Alves1Marina T. F. Dellias2Acacio A. Navarrete3Thiago O. Basso4Siu M. Tsai5Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture (CENA), University of São Paulo (USP)Brazilian Bioethanol Science and Technology Laboratory (CTBE), University of Campinas (Unicamp)Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture (CENA), University of São Paulo (USP)Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture (CENA), University of São Paulo (USP)Department of Chemical Engineering, Polytechnic School, University of São PauloCell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture (CENA), University of São Paulo (USP)Abstract Every year around 300 Gl of vinasse, a by-product of ethanol distillation in sugarcane mills, are flushed into more than 9 Mha of sugarcane cropland in Brazil. This practice links fermentation waste management to fertilization for plant biomass production, and it is known as fertirrigation. Here we evaluate public datasets of soil metagenomes mining for changes in antibiotic resistance genes (ARGs) of soils from sugarcane mesocosms repeatedly amended with vinasse. The metagenomes were annotated using the ResFam database. We found that the abundance of open read frames (ORFs) annotated as ARGs changed significantly across 43 different families (p-value < 0.05). Co-occurrence network analysis revealed distinct patterns of interactions among ARGs, suggesting that nutrient amendment to soil microbial communities can impact on the coevolutionary dynamics of indigenous ARGs within soil resistome.http://link.springer.com/article/10.1186/s13040-017-0138-4SugarcaneVinasseResistomeAntibioticsResistance genesMetagenomic profiles |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lucas P. P. Braga Rafael F. Alves Marina T. F. Dellias Acacio A. Navarrete Thiago O. Basso Siu M. Tsai |
spellingShingle |
Lucas P. P. Braga Rafael F. Alves Marina T. F. Dellias Acacio A. Navarrete Thiago O. Basso Siu M. Tsai Vinasse fertirrigation alters soil resistome dynamics: an analysis based on metagenomic profiles BioData Mining Sugarcane Vinasse Resistome Antibiotics Resistance genes Metagenomic profiles |
author_facet |
Lucas P. P. Braga Rafael F. Alves Marina T. F. Dellias Acacio A. Navarrete Thiago O. Basso Siu M. Tsai |
author_sort |
Lucas P. P. Braga |
title |
Vinasse fertirrigation alters soil resistome dynamics: an analysis based on metagenomic profiles |
title_short |
Vinasse fertirrigation alters soil resistome dynamics: an analysis based on metagenomic profiles |
title_full |
Vinasse fertirrigation alters soil resistome dynamics: an analysis based on metagenomic profiles |
title_fullStr |
Vinasse fertirrigation alters soil resistome dynamics: an analysis based on metagenomic profiles |
title_full_unstemmed |
Vinasse fertirrigation alters soil resistome dynamics: an analysis based on metagenomic profiles |
title_sort |
vinasse fertirrigation alters soil resistome dynamics: an analysis based on metagenomic profiles |
publisher |
BMC |
series |
BioData Mining |
issn |
1756-0381 |
publishDate |
2017-05-01 |
description |
Abstract Every year around 300 Gl of vinasse, a by-product of ethanol distillation in sugarcane mills, are flushed into more than 9 Mha of sugarcane cropland in Brazil. This practice links fermentation waste management to fertilization for plant biomass production, and it is known as fertirrigation. Here we evaluate public datasets of soil metagenomes mining for changes in antibiotic resistance genes (ARGs) of soils from sugarcane mesocosms repeatedly amended with vinasse. The metagenomes were annotated using the ResFam database. We found that the abundance of open read frames (ORFs) annotated as ARGs changed significantly across 43 different families (p-value < 0.05). Co-occurrence network analysis revealed distinct patterns of interactions among ARGs, suggesting that nutrient amendment to soil microbial communities can impact on the coevolutionary dynamics of indigenous ARGs within soil resistome. |
topic |
Sugarcane Vinasse Resistome Antibiotics Resistance genes Metagenomic profiles |
url |
http://link.springer.com/article/10.1186/s13040-017-0138-4 |
work_keys_str_mv |
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