Distribution of 18S rDNA clusters in Central European harvestmen of the suborder Eupnoi (Arachnida: Opiliones)
rDNA clusters are an important cytogenetic marker for studying karyotype evolution and chromosomal changes. The variability of this cytogenetic characteristic is, however, still almost unknown in the karyotypes of the entire class Arachnida (Arthropoda: Chelicerata). This situation is particularly e...
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Institute of Entomology, Biology Centre, Czech Academy of Science
2020-06-01
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doaj-4b983b85cb764fb2981bd85095bee8a22021-04-16T20:36:13ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292020-06-01117128228810.14411/eje.2020.032eje-202001-0032Distribution of 18S rDNA clusters in Central European harvestmen of the suborder Eupnoi (Arachnida: Opiliones)Hana JINDROVÁ0Matyáš HIŘMAN1David SADÍLEK2Pavel BEZDĚČKA3František ŠŤÁHLAVSKÝ4Charles University, Faculty of Science, Department of Zoology, Viničná 7, CZ-12844 Praha, Czech Republic; e-mails: svojanovska.hanka@seznam.cz, hirmanm@natur.cuni.cz, david.sadilek@natur.cuni.cz, frantisek.stahlavsky@natur.cuni.czCharles University, Faculty of Science, Department of Zoology, Viničná 7, CZ-12844 Praha, Czech Republic; e-mails: svojanovska.hanka@seznam.cz, hirmanm@natur.cuni.cz, david.sadilek@natur.cuni.cz, frantisek.stahlavsky@natur.cuni.czCharles University, Faculty of Science, Department of Zoology, Viničná 7, CZ-12844 Praha, Czech Republic; e-mails: svojanovska.hanka@seznam.cz, hirmanm@natur.cuni.cz, david.sadilek@natur.cuni.cz, frantisek.stahlavsky@natur.cuni.czMuseum of the Highlands Jihlava, Masarykovo náměstí 55, CZ-58601 Jihlava, Czech Republic; e-mail: bezdecka@muzeum.ji.czCharles University, Faculty of Science, Department of Zoology, Viničná 7, CZ-12844 Praha, Czech Republic; e-mails: svojanovska.hanka@seznam.cz, hirmanm@natur.cuni.cz, david.sadilek@natur.cuni.cz, frantisek.stahlavsky@natur.cuni.czrDNA clusters are an important cytogenetic marker for studying karyotype evolution and chromosomal changes. The variability of this cytogenetic characteristic is, however, still almost unknown in the karyotypes of the entire class Arachnida (Arthropoda: Chelicerata). This situation is particularly evident in harvestmen (Arachnida: Opiliones), with 97 species studied cytogenetically, for which there is information on the number and position of rDNA clusters for only 13. Moreover, previous studies indicate that the number of rDNA loci is highly variable in the species analysed, ranging from one to five pairs of rDNA clusters. Based on this fragmentary information, which is for rare species mainly from the limits of the distribution of their families, it is still not possible to reconstruct the ancestral state for this important cytogenetic feature in this order. Building upon recent research in Central Europe, we analysed the number and position of 18S rDNA in 13 species belonging to the suborder Eupnoi. This revealed that their karyotypes were variable in terms of the diploid number (2n = 16-36) and number of 18S rDNA clusters (from one to seven pairs). For the first time, an 18S rDNA cluster was detected on B chromosomes in harvestmen. Our study sheds new light on the karyotype evolution and 18S rDNA distribution in harvestmen and provides an improved understanding of the ancestral state of karyotypes in the order Opiliones.https://www.eje.cz/artkey/eje-202001-0032_distribution_of_18s_rdna_clusters_in_central_european_harvestmen_of_the_suborder_eupnoi_arachnida_opiliones.phpopilionessclerosomatidaephalangiidaekaryotypefishnorsmeiosis18s rdna |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hana JINDROVÁ Matyáš HIŘMAN David SADÍLEK Pavel BEZDĚČKA František ŠŤÁHLAVSKÝ |
spellingShingle |
Hana JINDROVÁ Matyáš HIŘMAN David SADÍLEK Pavel BEZDĚČKA František ŠŤÁHLAVSKÝ Distribution of 18S rDNA clusters in Central European harvestmen of the suborder Eupnoi (Arachnida: Opiliones) European Journal of Entomology opiliones sclerosomatidae phalangiidae karyotype fish nors meiosis 18s rdna |
author_facet |
Hana JINDROVÁ Matyáš HIŘMAN David SADÍLEK Pavel BEZDĚČKA František ŠŤÁHLAVSKÝ |
author_sort |
Hana JINDROVÁ |
title |
Distribution of 18S rDNA clusters in Central European harvestmen of the suborder Eupnoi (Arachnida: Opiliones) |
title_short |
Distribution of 18S rDNA clusters in Central European harvestmen of the suborder Eupnoi (Arachnida: Opiliones) |
title_full |
Distribution of 18S rDNA clusters in Central European harvestmen of the suborder Eupnoi (Arachnida: Opiliones) |
title_fullStr |
Distribution of 18S rDNA clusters in Central European harvestmen of the suborder Eupnoi (Arachnida: Opiliones) |
title_full_unstemmed |
Distribution of 18S rDNA clusters in Central European harvestmen of the suborder Eupnoi (Arachnida: Opiliones) |
title_sort |
distribution of 18s rdna clusters in central european harvestmen of the suborder eupnoi (arachnida: opiliones) |
publisher |
Institute of Entomology, Biology Centre, Czech Academy of Science |
series |
European Journal of Entomology |
issn |
1210-5759 1802-8829 |
publishDate |
2020-06-01 |
description |
rDNA clusters are an important cytogenetic marker for studying karyotype evolution and chromosomal changes. The variability of this cytogenetic characteristic is, however, still almost unknown in the karyotypes of the entire class Arachnida (Arthropoda: Chelicerata). This situation is particularly evident in harvestmen (Arachnida: Opiliones), with 97 species studied cytogenetically, for which there is information on the number and position of rDNA clusters for only 13. Moreover, previous studies indicate that the number of rDNA loci is highly variable in the species analysed, ranging from one to five pairs of rDNA clusters. Based on this fragmentary information, which is for rare species mainly from the limits of the distribution of their families, it is still not possible to reconstruct the ancestral state for this important cytogenetic feature in this order. Building upon recent research in Central Europe, we analysed the number and position of 18S rDNA in 13 species belonging to the suborder Eupnoi. This revealed that their karyotypes were variable in terms of the diploid number (2n = 16-36) and number of 18S rDNA clusters (from one to seven pairs). For the first time, an 18S rDNA cluster was detected on B chromosomes in harvestmen. Our study sheds new light on the karyotype evolution and 18S rDNA distribution in harvestmen and provides an improved understanding of the ancestral state of karyotypes in the order Opiliones. |
topic |
opiliones sclerosomatidae phalangiidae karyotype fish nors meiosis 18s rdna |
url |
https://www.eje.cz/artkey/eje-202001-0032_distribution_of_18s_rdna_clusters_in_central_european_harvestmen_of_the_suborder_eupnoi_arachnida_opiliones.php |
work_keys_str_mv |
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