An optimized microwave-assisted extraction method for increasing yields of rare ginsenosides from Panax quinquefolius L.
Background: Rare ginsenosides in Panax quinquefolius L. have strong bioactivities. The fact that it is hard to obtain large amounts of rare ginsenosides seriously restricts further research on these compounds. An easy, fast, and efficient method to obtain different kinds of rare ginsenosides simulta...
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doaj-4bd97289be2f437d9c1f9d9b542a46142020-11-24T22:56:03ZengElsevierJournal of Ginseng Research1226-84532016-10-0140441542210.1016/j.jgr.2016.06.007An optimized microwave-assisted extraction method for increasing yields of rare ginsenosides from Panax quinquefolius L.Hua Yao0Xuwen Li1Ying Liu2Qian Wu3Yongri Jin4Scientific Research Center, China–Japan Union Hospital of Jilin University, Jilin, ChinaCollege of Chemistry, Jilin University, Jilin, ChinaCollege of Chemistry, Jilin University, Jilin, ChinaCollege of Chemistry, Jilin University, Jilin, ChinaCollege of Chemistry, Jilin University, Jilin, ChinaBackground: Rare ginsenosides in Panax quinquefolius L. have strong bioactivities. The fact that it is hard to obtain large amounts of rare ginsenosides seriously restricts further research on these compounds. An easy, fast, and efficient method to obtain different kinds of rare ginsenosides simultaneously and to quantify each one precisely is urgently needed. Methods: Microwave-assisted extraction (MAE) was used to extract nine kinds of rare ginsenosides from P. quinquefolius L. In this article, rare ginsenosides [20(S)-Rh1, 20(R)-Rh1, Rg6, F4, Rk3, 20(S)-Rg3, 20(R)-Rg3, Rk1, and Rg5] were identified by high performance liquid chromatography (HPLC)–electrospray ionization–mass spectrometry. The quantity information of rare ginsenosides was analyzed by HPLC-UV at 203 nm. Results: The optimal conditions for MAE were using water as solvent with the material ratio of 1:40 (w/v) at a temperature of 145°C, and extracting for 15 min under microwave power of 1,600 W. Seven kinds of rare ginsenosides [20(S)-Rh1, 20(R)-Rh1, Rg6, F4, Rk3, Rk1, and Rg5] had high extraction yields, but those of 20(S)-Rg3 and 20(R)-Rg3 were lower. Compared with the conventional method, the extraction yields of the nine rare ginsenosides were significantly increased. Conclusion: The results indicate that rare ginsenosides can be extracted effectively by MAE from P. quinquefolius L. in a short time. Microwave radiation plays an important role in MAE. The probable generation process of rare ginsenosides is also discussed in the article. It will be meaningful for further investigation or application of rare ginsenosides.http://www.sciencedirect.com/science/article/pii/S1226845316301191high performance liquid chromatography/mass spectrometrymicrowave-assisted extractionPanax quinquefolius L.rare ginsenosides |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hua Yao Xuwen Li Ying Liu Qian Wu Yongri Jin |
spellingShingle |
Hua Yao Xuwen Li Ying Liu Qian Wu Yongri Jin An optimized microwave-assisted extraction method for increasing yields of rare ginsenosides from Panax quinquefolius L. Journal of Ginseng Research high performance liquid chromatography/mass spectrometry microwave-assisted extraction Panax quinquefolius L. rare ginsenosides |
author_facet |
Hua Yao Xuwen Li Ying Liu Qian Wu Yongri Jin |
author_sort |
Hua Yao |
title |
An optimized microwave-assisted extraction method for increasing yields of rare ginsenosides from Panax quinquefolius L. |
title_short |
An optimized microwave-assisted extraction method for increasing yields of rare ginsenosides from Panax quinquefolius L. |
title_full |
An optimized microwave-assisted extraction method for increasing yields of rare ginsenosides from Panax quinquefolius L. |
title_fullStr |
An optimized microwave-assisted extraction method for increasing yields of rare ginsenosides from Panax quinquefolius L. |
title_full_unstemmed |
An optimized microwave-assisted extraction method for increasing yields of rare ginsenosides from Panax quinquefolius L. |
title_sort |
optimized microwave-assisted extraction method for increasing yields of rare ginsenosides from panax quinquefolius l. |
publisher |
Elsevier |
series |
Journal of Ginseng Research |
issn |
1226-8453 |
publishDate |
2016-10-01 |
description |
Background: Rare ginsenosides in Panax quinquefolius L. have strong bioactivities. The fact that it is hard to obtain large amounts of rare ginsenosides seriously restricts further research on these compounds. An easy, fast, and efficient method to obtain different kinds of rare ginsenosides simultaneously and to quantify each one precisely is urgently needed.
Methods: Microwave-assisted extraction (MAE) was used to extract nine kinds of rare ginsenosides from P. quinquefolius L. In this article, rare ginsenosides [20(S)-Rh1, 20(R)-Rh1, Rg6, F4, Rk3, 20(S)-Rg3, 20(R)-Rg3, Rk1, and Rg5] were identified by high performance liquid chromatography (HPLC)–electrospray ionization–mass spectrometry. The quantity information of rare ginsenosides was analyzed by HPLC-UV at 203 nm.
Results: The optimal conditions for MAE were using water as solvent with the material ratio of 1:40 (w/v) at a temperature of 145°C, and extracting for 15 min under microwave power of 1,600 W. Seven kinds of rare ginsenosides [20(S)-Rh1, 20(R)-Rh1, Rg6, F4, Rk3, Rk1, and Rg5] had high extraction yields, but those of 20(S)-Rg3 and 20(R)-Rg3 were lower. Compared with the conventional method, the extraction yields of the nine rare ginsenosides were significantly increased.
Conclusion: The results indicate that rare ginsenosides can be extracted effectively by MAE from P. quinquefolius L. in a short time. Microwave radiation plays an important role in MAE. The probable generation process of rare ginsenosides is also discussed in the article. It will be meaningful for further investigation or application of rare ginsenosides. |
topic |
high performance liquid chromatography/mass spectrometry microwave-assisted extraction Panax quinquefolius L. rare ginsenosides |
url |
http://www.sciencedirect.com/science/article/pii/S1226845316301191 |
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