KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light.
Genetically encoded photosensitizers, proteins that produce reactive oxygen species when illuminated with visible light, are increasingly used as optogenetic tools. Their applications range from ablation of specific cell populations to precise optical inactivation of cellular proteins. Here, we repo...
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doaj-4be45a6ed02044b89daa9fdc7e3683da2020-11-25T02:33:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011012e014528710.1371/journal.pone.0145287KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light.Karen S SarkisyanOlga A ZlobovskayaDmitry A GorbachevNina G BozhanovaGeorge V SharonovDmitriy B StaroverovEvgeny S EgorovAnastasia V RyabovaKyril M SolntsevAlexander S MishinKonstantin A LukyanovGenetically encoded photosensitizers, proteins that produce reactive oxygen species when illuminated with visible light, are increasingly used as optogenetic tools. Their applications range from ablation of specific cell populations to precise optical inactivation of cellular proteins. Here, we report an orange mutant of red fluorescent protein KillerRed that becomes toxic when illuminated with blue or green light. This new protein, KillerOrange, carries a tryptophan-based chromophore that is novel for photosensitizers. We show that KillerOrange can be used simultaneously and independently from KillerRed in both bacterial and mammalian cells offering chromatic orthogonality for light-activated toxicity.http://europepmc.org/articles/PMC4683004?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Karen S Sarkisyan Olga A Zlobovskaya Dmitry A Gorbachev Nina G Bozhanova George V Sharonov Dmitriy B Staroverov Evgeny S Egorov Anastasia V Ryabova Kyril M Solntsev Alexander S Mishin Konstantin A Lukyanov |
spellingShingle |
Karen S Sarkisyan Olga A Zlobovskaya Dmitry A Gorbachev Nina G Bozhanova George V Sharonov Dmitriy B Staroverov Evgeny S Egorov Anastasia V Ryabova Kyril M Solntsev Alexander S Mishin Konstantin A Lukyanov KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light. PLoS ONE |
author_facet |
Karen S Sarkisyan Olga A Zlobovskaya Dmitry A Gorbachev Nina G Bozhanova George V Sharonov Dmitriy B Staroverov Evgeny S Egorov Anastasia V Ryabova Kyril M Solntsev Alexander S Mishin Konstantin A Lukyanov |
author_sort |
Karen S Sarkisyan |
title |
KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light. |
title_short |
KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light. |
title_full |
KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light. |
title_fullStr |
KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light. |
title_full_unstemmed |
KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light. |
title_sort |
killerorange, a genetically encoded photosensitizer activated by blue and green light. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
Genetically encoded photosensitizers, proteins that produce reactive oxygen species when illuminated with visible light, are increasingly used as optogenetic tools. Their applications range from ablation of specific cell populations to precise optical inactivation of cellular proteins. Here, we report an orange mutant of red fluorescent protein KillerRed that becomes toxic when illuminated with blue or green light. This new protein, KillerOrange, carries a tryptophan-based chromophore that is novel for photosensitizers. We show that KillerOrange can be used simultaneously and independently from KillerRed in both bacterial and mammalian cells offering chromatic orthogonality for light-activated toxicity. |
url |
http://europepmc.org/articles/PMC4683004?pdf=render |
work_keys_str_mv |
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