Resistance of mice to infection with secondary hydatid disease by polyamine oxidase isomer

• Main Author: Wathba ُEdrees Tohala
• Format: Article
• Language: Arabic
• Published: College of Education for Pure Sciences 2013-06-01
• Series: مجلة التربية والعلم
• Subjects: mice; hydatid disease; polyamine oxidase; isomer
• Online Access: https://edusj.mosuljournals.com/article_89796_ba0bb978d3f8e503309428e56f5a505f.pdf

ABSTRACT<br /> Polyamine oxidase activity in cerebrospinal fluid of normal children was determined. The specific activity of crude PAO was found 64.16  8.18 enzyme unit/mg protein. Two main peaks of high PAO activities were obtained I and II with specific activity of 1201.9 and 1157.2 enzyme unit/mg protein, and with purification folds of 18.2 and 17.5 respectively.<br /> The resistance to infection with secondary hydatid disease was studied in mice activated by partially purified CSF-PAO with spermine and infected with protoscoleces of Echinococcus granulosus. <br /> The results revealed an obvious decrease in numbers, weights and diameters of secondary hydatid cysts grown, and elevation in the percentage of reduction of hydatid cysts, and a decrease in liver and spleen weights and their organ indices, in mice activated by different concentrations of PAO II 200-1000 µg/ 10 gm body weight with constant concentration 200 µg Spm throughout one month, and with optimum concentration 800 µg/ 10 gm body weight PAO II with 200 µg Spm throughout two and three months, in comparison with positive and negative control groups.<br /> Therefore, it was concluded that PAO II isolated from CSF with Spm could be considered as an effective resistance against infection with secondary hydatid disease.