Bee Venom Melittin Protects against Cisplatin-Induced Acute Kidney Injury in Mice via the Regulation of M2 Macrophage Activation
Inflammation is an essential biological response that eliminates pathogenic bacteria and repairs tissue after injury. Acute kidney injury (AKI) is associated with systemic and intrarenal inflammation as the inflammatory process decreases renal function and promotes progression to advanced chronic ki...
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doaj-4bee8c7a0beb473692ac57f60798d5e22020-11-25T03:07:24ZengMDPI AGToxins2072-66512020-09-011257457410.3390/toxins12090574Bee Venom Melittin Protects against Cisplatin-Induced Acute Kidney Injury in Mice via the Regulation of M2 Macrophage ActivationHyunseong Kim0Jin Young Hong1Wan-Jin Jeon2Seung Ho Baek3In-Hyuk Ha4Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation, Seoul 135-896, KoreaJaseng Spine and Joint Research Institute, Jaseng Medical Foundation, Seoul 135-896, KoreaJaseng Spine and Joint Research Institute, Jaseng Medical Foundation, Seoul 135-896, KoreaCollege of Korean Medicine, Dongguk University, 32 Dongguk-ro, Ilsandong-gu, Goyang-si 10326, Gyeonggi-do, KoreaJaseng Spine and Joint Research Institute, Jaseng Medical Foundation, Seoul 135-896, KoreaInflammation is an essential biological response that eliminates pathogenic bacteria and repairs tissue after injury. Acute kidney injury (AKI) is associated with systemic and intrarenal inflammation as the inflammatory process decreases renal function and promotes progression to advanced chronic kidney disease. Macrophages are key mediators of the inflammatory response; their activation influences the immune system and may have various effects. Classically activated type I macrophages (M1) produce a variety of pro-inflammatory cytokines at the lesion site. However, anti-inflammatory type II macrophages (M2) are alternatively activated upon exposure to anti-inflammatory cytokines and are associated with wound healing and tissue repair following AKI. Here, we used melittin from bee venom to enhance the polarization of M2 macrophages and promote renal recovery after AKI. Melittin was administered to mice intraperitoneally for 5 days at various concentrations (10, 50, and 100 µg/kg); serum creatinine and blood urea nitrogen (BUN) levels were analyzed 72 h after cisplatin administration to confirm renal dysfunction. Melittin inhibited the cisplatin-induced increase in creatinine and BUN, an indicator of renal dysfunction. The expression of M1 markers (CD16/32) decreased significantly, whereas that of M2 markers (CD206, Arg1nase I) increased after melittin administration. Consistently, tubular necrosis was substantially reduced in melittin-treated mice. Thus, melittin alleviates cisplatin-induced AKI by regulating M2 macrophage expression.https://www.mdpi.com/2072-6651/12/9/574cisplatinacute kidney injurymelittinM2 macrophage |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hyunseong Kim Jin Young Hong Wan-Jin Jeon Seung Ho Baek In-Hyuk Ha |
spellingShingle |
Hyunseong Kim Jin Young Hong Wan-Jin Jeon Seung Ho Baek In-Hyuk Ha Bee Venom Melittin Protects against Cisplatin-Induced Acute Kidney Injury in Mice via the Regulation of M2 Macrophage Activation Toxins cisplatin acute kidney injury melittin M2 macrophage |
author_facet |
Hyunseong Kim Jin Young Hong Wan-Jin Jeon Seung Ho Baek In-Hyuk Ha |
author_sort |
Hyunseong Kim |
title |
Bee Venom Melittin Protects against Cisplatin-Induced Acute Kidney Injury in Mice via the Regulation of M2 Macrophage Activation |
title_short |
Bee Venom Melittin Protects against Cisplatin-Induced Acute Kidney Injury in Mice via the Regulation of M2 Macrophage Activation |
title_full |
Bee Venom Melittin Protects against Cisplatin-Induced Acute Kidney Injury in Mice via the Regulation of M2 Macrophage Activation |
title_fullStr |
Bee Venom Melittin Protects against Cisplatin-Induced Acute Kidney Injury in Mice via the Regulation of M2 Macrophage Activation |
title_full_unstemmed |
Bee Venom Melittin Protects against Cisplatin-Induced Acute Kidney Injury in Mice via the Regulation of M2 Macrophage Activation |
title_sort |
bee venom melittin protects against cisplatin-induced acute kidney injury in mice via the regulation of m2 macrophage activation |
publisher |
MDPI AG |
series |
Toxins |
issn |
2072-6651 |
publishDate |
2020-09-01 |
description |
Inflammation is an essential biological response that eliminates pathogenic bacteria and repairs tissue after injury. Acute kidney injury (AKI) is associated with systemic and intrarenal inflammation as the inflammatory process decreases renal function and promotes progression to advanced chronic kidney disease. Macrophages are key mediators of the inflammatory response; their activation influences the immune system and may have various effects. Classically activated type I macrophages (M1) produce a variety of pro-inflammatory cytokines at the lesion site. However, anti-inflammatory type II macrophages (M2) are alternatively activated upon exposure to anti-inflammatory cytokines and are associated with wound healing and tissue repair following AKI. Here, we used melittin from bee venom to enhance the polarization of M2 macrophages and promote renal recovery after AKI. Melittin was administered to mice intraperitoneally for 5 days at various concentrations (10, 50, and 100 µg/kg); serum creatinine and blood urea nitrogen (BUN) levels were analyzed 72 h after cisplatin administration to confirm renal dysfunction. Melittin inhibited the cisplatin-induced increase in creatinine and BUN, an indicator of renal dysfunction. The expression of M1 markers (CD16/32) decreased significantly, whereas that of M2 markers (CD206, Arg1nase I) increased after melittin administration. Consistently, tubular necrosis was substantially reduced in melittin-treated mice. Thus, melittin alleviates cisplatin-induced AKI by regulating M2 macrophage expression. |
topic |
cisplatin acute kidney injury melittin M2 macrophage |
url |
https://www.mdpi.com/2072-6651/12/9/574 |
work_keys_str_mv |
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