Rhodotorula Strains Isolated from Seawater That Can Biotransform Raw Glycerol into Docosahexaenoic Acid (DHA) and Carotenoids for Animal Nutrition
Due to the overexploitation of industrial fisheries, as the principal source of fish oil, as well as the increasing replacement of synthetic pigments for animal nutrition, we need to find sustainable sources for these essential nutrient productions. Marine <i>Rhodotorula</i> strains NCYC...
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doaj-4bf178e6018340bb812053ece31c67862021-03-23T00:02:31ZengMDPI AGApplied Sciences2076-34172021-03-01112824282410.3390/app11062824Rhodotorula Strains Isolated from Seawater That Can Biotransform Raw Glycerol into Docosahexaenoic Acid (DHA) and Carotenoids for Animal NutritionNatalie L. Pino-Maureira0Rodrigo R. González-Saldía1Alejandro Capdeville2Benjamín Srain3Doctorado en Ciencias con Mención en Manejo de Recursos Acuáticos Renovables (MaReA), Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Concepción 4070405, ChileDoctorado en Ciencias con Mención en Manejo de Recursos Acuáticos Renovables (MaReA), Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Concepción 4070405, ChileEWOS Chile, Alimentos Ltd.a., Parque Industrial, Escuadrón Km 20, Coronel 4190000, ChileCentro de Investigación Oceanográfica COPAS Sur-Austral, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Concepción 4070405, ChileDue to the overexploitation of industrial fisheries, as the principal source of fish oil, as well as the increasing replacement of synthetic pigments for animal nutrition, we need to find sustainable sources for these essential nutrient productions. Marine <i>Rhodotorula</i> strains NCYC4007 and NCYC1146 were used to determine the biosynthesis of docosahexaenoic acid (DHA) and carotenoids by biotransforming raw glycerol, a waste product of biodiesel. To evaluate the presence of inhibitory substances in raw glycerol, both strains were also grown in the presence of analytical grade glycerol and glucose as the main carbon source separately. With raw glycerol, NCYC4007 showed a significant correlation between DHA production and intracellular phosphorous concentrations. NCYC1146, a new <i>Rhodotorula</i> strain genetically described in this work, can produce canthaxanthin but only when glycerol is used as a main carbon source. Then, NCYC4007 could synthesize DHA as a phospholipid, and the production of canthaxanthin depends on the kind of carbon source used by NCYC1146. Finally, malate dehydrogenase activity and glucose production can be used as a proxy of the metabolisms in these marine <i>Rhodotorula</i>. This is the first evidence that marine <i>Rhodotorula</i> are capable of synthesizing DHA and canthaxanthin using an alternative and low-cost source of carbon to potentially scale their sustainable production for animal nutrition.https://www.mdpi.com/2076-3417/11/6/2824<i>Rhodotorula </i>sp.docosahexaenoic acid (DHA)carotenoidscanthaxanthinraw glycerol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Natalie L. Pino-Maureira Rodrigo R. González-Saldía Alejandro Capdeville Benjamín Srain |
spellingShingle |
Natalie L. Pino-Maureira Rodrigo R. González-Saldía Alejandro Capdeville Benjamín Srain Rhodotorula Strains Isolated from Seawater That Can Biotransform Raw Glycerol into Docosahexaenoic Acid (DHA) and Carotenoids for Animal Nutrition Applied Sciences <i>Rhodotorula </i>sp. docosahexaenoic acid (DHA) carotenoids canthaxanthin raw glycerol |
author_facet |
Natalie L. Pino-Maureira Rodrigo R. González-Saldía Alejandro Capdeville Benjamín Srain |
author_sort |
Natalie L. Pino-Maureira |
title |
Rhodotorula Strains Isolated from Seawater That Can Biotransform Raw Glycerol into Docosahexaenoic Acid (DHA) and Carotenoids for Animal Nutrition |
title_short |
Rhodotorula Strains Isolated from Seawater That Can Biotransform Raw Glycerol into Docosahexaenoic Acid (DHA) and Carotenoids for Animal Nutrition |
title_full |
Rhodotorula Strains Isolated from Seawater That Can Biotransform Raw Glycerol into Docosahexaenoic Acid (DHA) and Carotenoids for Animal Nutrition |
title_fullStr |
Rhodotorula Strains Isolated from Seawater That Can Biotransform Raw Glycerol into Docosahexaenoic Acid (DHA) and Carotenoids for Animal Nutrition |
title_full_unstemmed |
Rhodotorula Strains Isolated from Seawater That Can Biotransform Raw Glycerol into Docosahexaenoic Acid (DHA) and Carotenoids for Animal Nutrition |
title_sort |
rhodotorula strains isolated from seawater that can biotransform raw glycerol into docosahexaenoic acid (dha) and carotenoids for animal nutrition |
publisher |
MDPI AG |
series |
Applied Sciences |
issn |
2076-3417 |
publishDate |
2021-03-01 |
description |
Due to the overexploitation of industrial fisheries, as the principal source of fish oil, as well as the increasing replacement of synthetic pigments for animal nutrition, we need to find sustainable sources for these essential nutrient productions. Marine <i>Rhodotorula</i> strains NCYC4007 and NCYC1146 were used to determine the biosynthesis of docosahexaenoic acid (DHA) and carotenoids by biotransforming raw glycerol, a waste product of biodiesel. To evaluate the presence of inhibitory substances in raw glycerol, both strains were also grown in the presence of analytical grade glycerol and glucose as the main carbon source separately. With raw glycerol, NCYC4007 showed a significant correlation between DHA production and intracellular phosphorous concentrations. NCYC1146, a new <i>Rhodotorula</i> strain genetically described in this work, can produce canthaxanthin but only when glycerol is used as a main carbon source. Then, NCYC4007 could synthesize DHA as a phospholipid, and the production of canthaxanthin depends on the kind of carbon source used by NCYC1146. Finally, malate dehydrogenase activity and glucose production can be used as a proxy of the metabolisms in these marine <i>Rhodotorula</i>. This is the first evidence that marine <i>Rhodotorula</i> are capable of synthesizing DHA and canthaxanthin using an alternative and low-cost source of carbon to potentially scale their sustainable production for animal nutrition. |
topic |
<i>Rhodotorula </i>sp. docosahexaenoic acid (DHA) carotenoids canthaxanthin raw glycerol |
url |
https://www.mdpi.com/2076-3417/11/6/2824 |
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