Proteomic analysis of buccal gland secretion from fasting and feeding lampreys (Lampetra morii)
Abstract Background Previous studies have shown that lamprey buccal glands contain some regulators related to anticoagulation, nociception, and immune responses due to the blood sucking habit. Regrettably, the protein expression profile in the buccal glands of feeding lampreys has never been reporte...
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doaj-4c13e3295827406a89c74f2b9c2e05822020-11-24T21:29:05ZengBMCProteome Science1477-59562018-05-011611910.1186/s12953-018-0137-5Proteomic analysis of buccal gland secretion from fasting and feeding lampreys (Lampetra morii)Bowen Li0Meng Gou1Jianmei Han2Xiaofei Yuan3Yingying Li4Tiesong Li5Qi Jiang6Rong Xiao7Qingwei Li8School of Life Sciences, Liaoning Normal UniversitySchool of Life Sciences, Liaoning Normal UniversitySchool of Life Sciences, Liaoning Normal UniversitySchool of Life Sciences, Liaoning Normal UniversitySchool of Life Sciences, Liaoning Normal UniversitySchool of Life Sciences, Liaoning Normal UniversitySchool of Life Sciences, Liaoning Normal UniversitySchool of Life Sciences, Liaoning Normal UniversitySchool of Life Sciences, Liaoning Normal UniversityAbstract Background Previous studies have shown that lamprey buccal glands contain some regulators related to anticoagulation, nociception, and immune responses due to the blood sucking habit. Regrettably, the protein expression profile in the buccal glands of feeding lampreys has never been reported yet. The present study was performed in order to further identify more proteins which are closely associated with lamprey feeding process. Methods 2D-PAGE, NanoLC–MS/MS with higher resolution, Ensembl lamprey and NCBI protein databases, as well as western blot was used to compare the proteomics of buccal gland secretion from China northeast lampreys (Lampetra morii) which had been fed for 0, 10, and 60 min, respectively. Results In the present study, the number of identified protein species in the buccal glands of feeding groups (60 min) was increased significantly, nearly ten times of that in the fasting group. During the feeding stage, novel proteins emerged in the buccal gland secretion of lampreys. According to gene ontology (GO) analysis and function predictions, these proteins were summarized and discussed based on their potential roles during feeding process. Furthermore, some of the identified proteins were confirmed to express during the feeding time of lampreys. Conclusion When lampreys attack host fishes to suck blood and flesh, their buccal glands could secrete enough proteins to suppress blood coagulation, nociception, oxidative stress, immune response, as well as other adverse effects encountered during their parasitic lives. The present study would provide clues to clarify the feeding mechanism of the bloodsucking lampreys.http://link.springer.com/article/10.1186/s12953-018-0137-5LampreysBuccal gland secretionFastingFeedingProteomic analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bowen Li Meng Gou Jianmei Han Xiaofei Yuan Yingying Li Tiesong Li Qi Jiang Rong Xiao Qingwei Li |
spellingShingle |
Bowen Li Meng Gou Jianmei Han Xiaofei Yuan Yingying Li Tiesong Li Qi Jiang Rong Xiao Qingwei Li Proteomic analysis of buccal gland secretion from fasting and feeding lampreys (Lampetra morii) Proteome Science Lampreys Buccal gland secretion Fasting Feeding Proteomic analysis |
author_facet |
Bowen Li Meng Gou Jianmei Han Xiaofei Yuan Yingying Li Tiesong Li Qi Jiang Rong Xiao Qingwei Li |
author_sort |
Bowen Li |
title |
Proteomic analysis of buccal gland secretion from fasting and feeding lampreys (Lampetra morii) |
title_short |
Proteomic analysis of buccal gland secretion from fasting and feeding lampreys (Lampetra morii) |
title_full |
Proteomic analysis of buccal gland secretion from fasting and feeding lampreys (Lampetra morii) |
title_fullStr |
Proteomic analysis of buccal gland secretion from fasting and feeding lampreys (Lampetra morii) |
title_full_unstemmed |
Proteomic analysis of buccal gland secretion from fasting and feeding lampreys (Lampetra morii) |
title_sort |
proteomic analysis of buccal gland secretion from fasting and feeding lampreys (lampetra morii) |
publisher |
BMC |
series |
Proteome Science |
issn |
1477-5956 |
publishDate |
2018-05-01 |
description |
Abstract Background Previous studies have shown that lamprey buccal glands contain some regulators related to anticoagulation, nociception, and immune responses due to the blood sucking habit. Regrettably, the protein expression profile in the buccal glands of feeding lampreys has never been reported yet. The present study was performed in order to further identify more proteins which are closely associated with lamprey feeding process. Methods 2D-PAGE, NanoLC–MS/MS with higher resolution, Ensembl lamprey and NCBI protein databases, as well as western blot was used to compare the proteomics of buccal gland secretion from China northeast lampreys (Lampetra morii) which had been fed for 0, 10, and 60 min, respectively. Results In the present study, the number of identified protein species in the buccal glands of feeding groups (60 min) was increased significantly, nearly ten times of that in the fasting group. During the feeding stage, novel proteins emerged in the buccal gland secretion of lampreys. According to gene ontology (GO) analysis and function predictions, these proteins were summarized and discussed based on their potential roles during feeding process. Furthermore, some of the identified proteins were confirmed to express during the feeding time of lampreys. Conclusion When lampreys attack host fishes to suck blood and flesh, their buccal glands could secrete enough proteins to suppress blood coagulation, nociception, oxidative stress, immune response, as well as other adverse effects encountered during their parasitic lives. The present study would provide clues to clarify the feeding mechanism of the bloodsucking lampreys. |
topic |
Lampreys Buccal gland secretion Fasting Feeding Proteomic analysis |
url |
http://link.springer.com/article/10.1186/s12953-018-0137-5 |
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