In vivo magnetic resonance imaging tracking of C6 glioma cells labeled with superparamagnetic iron oxide nanoparticles

Objective: The aim of the current study was to monitor the migrationof superparamagnetic iron oxide nanoparticle (SPION)-labeled C6cells, which were used to induce glioblastoma tumor growth in ananimal model, over time using magnetic resonance imaging (MRI),with the goal of aiding in tumor prognosis...

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Main Authors: Javier Bustamante Mamani, Jackeline Moraes Malheiros, Ellison Fernando Cardoso, Alberto Tannús, Paulo Henrique Silveira, Lionel Fernel Gamarra
Format: Article
Language:English
Published: Instituto Israelita de Ensino e Pesquisa Albert Einstein 2012-06-01
Series:Einstein (São Paulo)
Subjects:
Online Access:http://apps.einstein.br/revista/arquivos/PDF/2418-164-170.pdf
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spelling doaj-4c1ddb0b6ff8471ead52f31e4d54e48a2020-11-24T22:50:26ZengInstituto Israelita de Ensino e Pesquisa Albert EinsteinEinstein (São Paulo)1679-45082012-06-01102164170In vivo magnetic resonance imaging tracking of C6 glioma cells labeled with superparamagnetic iron oxide nanoparticlesJavier Bustamante MamaniJackeline Moraes MalheirosEllison Fernando CardosoAlberto TannúsPaulo Henrique SilveiraLionel Fernel GamarraObjective: The aim of the current study was to monitor the migrationof superparamagnetic iron oxide nanoparticle (SPION)-labeled C6cells, which were used to induce glioblastoma tumor growth in ananimal model, over time using magnetic resonance imaging (MRI),with the goal of aiding in tumor prognosis and therapy. Methods: Twogroups of male Wistar rats were used for the tumor induction model.In the first group (n=3), the tumors were induced via the injectionof SPION-labeled C6 cells. In the second group (n=3), the tumorswere induced via the injection of unlabeled C6 cells. Prussian Bluestaining was performed to analyze the SPION distribution within theC6 cells in vitro. Tumor-inducing C6 cells were injected into the rightfrontal cortex, and subsequent tumor monitoring and SPION detectionwere performed using T2- and T2*-weighted MRI at a 2T fieldstrength. In addition, cancerous tissue was histologically analyzedafter performing the MRI studies. Results: The in vitro qualitativeevaluation demonstrated adequate distribution and satisfactory celllabeling of the SPIONs. At 14 or 21 days after C6 injection, a SPIONinducedT2- and T2*-weighted MRI signal reduction was observedwithin the lesion located in the left frontal lobe on parasagittaltopography. Moreover, histological staining of the tumor tissue withPrussian Blue revealed a broad distribution of SPIONs within the C6cells. Conclusion: MRI analyses exhibit potential for monitoring thetumor growth of C6 cells efficiently labeled with SPIONs.http://apps.einstein.br/revista/arquivos/PDF/2418-164-170.pdfGliomaNanoparticlesMagnetic resonance imagingAnimal models
collection DOAJ
language English
format Article
sources DOAJ
author Javier Bustamante Mamani
Jackeline Moraes Malheiros
Ellison Fernando Cardoso
Alberto Tannús
Paulo Henrique Silveira
Lionel Fernel Gamarra
spellingShingle Javier Bustamante Mamani
Jackeline Moraes Malheiros
Ellison Fernando Cardoso
Alberto Tannús
Paulo Henrique Silveira
Lionel Fernel Gamarra
In vivo magnetic resonance imaging tracking of C6 glioma cells labeled with superparamagnetic iron oxide nanoparticles
Einstein (São Paulo)
Glioma
Nanoparticles
Magnetic resonance imaging
Animal models
author_facet Javier Bustamante Mamani
Jackeline Moraes Malheiros
Ellison Fernando Cardoso
Alberto Tannús
Paulo Henrique Silveira
Lionel Fernel Gamarra
author_sort Javier Bustamante Mamani
title In vivo magnetic resonance imaging tracking of C6 glioma cells labeled with superparamagnetic iron oxide nanoparticles
title_short In vivo magnetic resonance imaging tracking of C6 glioma cells labeled with superparamagnetic iron oxide nanoparticles
title_full In vivo magnetic resonance imaging tracking of C6 glioma cells labeled with superparamagnetic iron oxide nanoparticles
title_fullStr In vivo magnetic resonance imaging tracking of C6 glioma cells labeled with superparamagnetic iron oxide nanoparticles
title_full_unstemmed In vivo magnetic resonance imaging tracking of C6 glioma cells labeled with superparamagnetic iron oxide nanoparticles
title_sort in vivo magnetic resonance imaging tracking of c6 glioma cells labeled with superparamagnetic iron oxide nanoparticles
publisher Instituto Israelita de Ensino e Pesquisa Albert Einstein
series Einstein (São Paulo)
issn 1679-4508
publishDate 2012-06-01
description Objective: The aim of the current study was to monitor the migrationof superparamagnetic iron oxide nanoparticle (SPION)-labeled C6cells, which were used to induce glioblastoma tumor growth in ananimal model, over time using magnetic resonance imaging (MRI),with the goal of aiding in tumor prognosis and therapy. Methods: Twogroups of male Wistar rats were used for the tumor induction model.In the first group (n=3), the tumors were induced via the injectionof SPION-labeled C6 cells. In the second group (n=3), the tumorswere induced via the injection of unlabeled C6 cells. Prussian Bluestaining was performed to analyze the SPION distribution within theC6 cells in vitro. Tumor-inducing C6 cells were injected into the rightfrontal cortex, and subsequent tumor monitoring and SPION detectionwere performed using T2- and T2*-weighted MRI at a 2T fieldstrength. In addition, cancerous tissue was histologically analyzedafter performing the MRI studies. Results: The in vitro qualitativeevaluation demonstrated adequate distribution and satisfactory celllabeling of the SPIONs. At 14 or 21 days after C6 injection, a SPIONinducedT2- and T2*-weighted MRI signal reduction was observedwithin the lesion located in the left frontal lobe on parasagittaltopography. Moreover, histological staining of the tumor tissue withPrussian Blue revealed a broad distribution of SPIONs within the C6cells. Conclusion: MRI analyses exhibit potential for monitoring thetumor growth of C6 cells efficiently labeled with SPIONs.
topic Glioma
Nanoparticles
Magnetic resonance imaging
Animal models
url http://apps.einstein.br/revista/arquivos/PDF/2418-164-170.pdf
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