Mechanisms of microtubule dynamics and force generation examined with computational modeling and electron cryotomography

Microtubules are dynamic tubulin polymers which elongate by addition of bent guanosine triphosphate tubulin to the tips of curving protofilaments. Here authors use Brownian dynamics modeling and electron cryotomography to show that the lateral activation energy barrier in tubulin-tubulin interaction...

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Main Authors:	Nikita B. Gudimchuk, Evgeni V. Ulyanov, Eileen O’Toole, Cynthia L. Page, Dmitrii S. Vinogradov, Garry Morgan, Gabriella Li, Jeffrey K. Moore, Ewa Szczesna, Antonina Roll-Mecak, Fazoil I. Ataullakhanov, J. Richard McIntosh
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-07-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-17553-2

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spelling	doaj-4c2a69ba2077454289ab669fcc7b56862021-08-01T11:40:05ZengNature Publishing GroupNature Communications2041-17232020-07-0111111510.1038/s41467-020-17553-2Mechanisms of microtubule dynamics and force generation examined with computational modeling and electron cryotomographyNikita B. Gudimchuk0Evgeni V. Ulyanov1Eileen O’Toole2Cynthia L. Page3Dmitrii S. Vinogradov4Garry Morgan5Gabriella Li6Jeffrey K. Moore7Ewa Szczesna8Antonina Roll-Mecak9Fazoil I. Ataullakhanov10J. Richard McIntosh11Department of Physics, Lomonosov Moscow State UniversityDepartment of Physics, Lomonosov Moscow State UniversityDepartment of Molecular, Cellular, and Developmental Biology, University of ColoradoDepartment of Molecular, Cellular, and Developmental Biology, University of ColoradoCenter for Theoretical Problems of Physicochemical Pharmacology, Russian Academy of SciencesDepartment of Molecular, Cellular, and Developmental Biology, University of ColoradoDepartment of Cell and Developmental Biology, University of Colorado School of MedicineDepartment of Cell and Developmental Biology, University of Colorado School of MedicineCell Biology and Biophysics Unit, National Institute of Neurological Disorders and StrokeCell Biology and Biophysics Unit, National Institute of Neurological Disorders and StrokeDepartment of Physics, Lomonosov Moscow State UniversityDepartment of Molecular, Cellular, and Developmental Biology, University of ColoradoMicrotubules are dynamic tubulin polymers which elongate by addition of bent guanosine triphosphate tubulin to the tips of curving protofilaments. Here authors use Brownian dynamics modeling and electron cryotomography to show that the lateral activation energy barrier in tubulin-tubulin interactions is a key parameter for this process, controlling the development of high pulling forces.https://doi.org/10.1038/s41467-020-17553-2
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Nikita B. Gudimchuk Evgeni V. Ulyanov Eileen O’Toole Cynthia L. Page Dmitrii S. Vinogradov Garry Morgan Gabriella Li Jeffrey K. Moore Ewa Szczesna Antonina Roll-Mecak Fazoil I. Ataullakhanov J. Richard McIntosh
spellingShingle	Nikita B. Gudimchuk Evgeni V. Ulyanov Eileen O’Toole Cynthia L. Page Dmitrii S. Vinogradov Garry Morgan Gabriella Li Jeffrey K. Moore Ewa Szczesna Antonina Roll-Mecak Fazoil I. Ataullakhanov J. Richard McIntosh Mechanisms of microtubule dynamics and force generation examined with computational modeling and electron cryotomography Nature Communications
author_facet	Nikita B. Gudimchuk Evgeni V. Ulyanov Eileen O’Toole Cynthia L. Page Dmitrii S. Vinogradov Garry Morgan Gabriella Li Jeffrey K. Moore Ewa Szczesna Antonina Roll-Mecak Fazoil I. Ataullakhanov J. Richard McIntosh
author_sort	Nikita B. Gudimchuk
title	Mechanisms of microtubule dynamics and force generation examined with computational modeling and electron cryotomography
title_short	Mechanisms of microtubule dynamics and force generation examined with computational modeling and electron cryotomography
title_full	Mechanisms of microtubule dynamics and force generation examined with computational modeling and electron cryotomography
title_fullStr	Mechanisms of microtubule dynamics and force generation examined with computational modeling and electron cryotomography
title_full_unstemmed	Mechanisms of microtubule dynamics and force generation examined with computational modeling and electron cryotomography
title_sort	mechanisms of microtubule dynamics and force generation examined with computational modeling and electron cryotomography
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2020-07-01
description	Microtubules are dynamic tubulin polymers which elongate by addition of bent guanosine triphosphate tubulin to the tips of curving protofilaments. Here authors use Brownian dynamics modeling and electron cryotomography to show that the lateral activation energy barrier in tubulin-tubulin interactions is a key parameter for this process, controlling the development of high pulling forces.
url	https://doi.org/10.1038/s41467-020-17553-2
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Mechanisms of microtubule dynamics and force generation examined with computational modeling and electron cryotomography

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