Evaluation of 99mTc-HYNIC-βAla-Bombesin(7-14) as an agent for pancreas tumor detection in mice
Pancreatic adenocarcinoma is important in oncology because of its high mortality rate. Deaths may be avoided if an early diagnosis could be achieved. Several types of tumors overexpress gastrin-releasing peptide receptors (GRPr), including pancreatic cancer cells. Thus, a radiolabeled peptide deriva...
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doaj-4c33cefd4ca044e38c07eb89bfecca662020-11-24T23:07:44ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2015-01-010000010.1590/1414-431X20154506S0100-879X2015005054506Evaluation of 99mTc-HYNIC-βAla-Bombesin(7-14) as an agent for pancreas tumor detection in miceF.N. CarlessoL.L. FuscaldiR.S. AraújoC.S. TeixeiraM.C. OliveiraS.O.A. FernandesG.D. CassaliD.C. ReisA.L.B. BarrosV.N. CardosoPancreatic adenocarcinoma is important in oncology because of its high mortality rate. Deaths may be avoided if an early diagnosis could be achieved. Several types of tumors overexpress gastrin-releasing peptide receptors (GRPr), including pancreatic cancer cells. Thus, a radiolabeled peptide derivative of gastrin-releasing peptide (GRP) may be useful as a specific imaging probe. The purpose of the present study was to evaluate the feasibility of using 99mTc-HYNIC-βAla-Bombesin(7-14) as an imaging probe for Capan-1 pancreatic adenocarcinoma. Xenographic pancreatic tumor was developed in nude mice and characterized by histopathological analysis. Biodistribution studies and scintigraphic images were carried out in tumor-bearing nude mice. The two methods showed higher uptake by pancreatic tumor when compared to muscle (used as control), and the tumor-to-muscle ratio indicated that 99mTc-HYNIC-βAla-Bombesin(7-14) uptake was four-fold higher in tumor cells than in other tissues. Scintigraphic images also showed a clear signal at the tumor site. The present data indicate that 99mTc-HYNIC-βAla-Bombesin(7-14) may be useful for the detection of pancreatic adenocarcinoma.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015005054506&lng=en&tlng=en99mTc-HYNIC-βAla-BombesinCapan-1Scintigraphic imagesPancreas tumorBiodistribution |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
F.N. Carlesso L.L. Fuscaldi R.S. Araújo C.S. Teixeira M.C. Oliveira S.O.A. Fernandes G.D. Cassali D.C. Reis A.L.B. Barros V.N. Cardoso |
spellingShingle |
F.N. Carlesso L.L. Fuscaldi R.S. Araújo C.S. Teixeira M.C. Oliveira S.O.A. Fernandes G.D. Cassali D.C. Reis A.L.B. Barros V.N. Cardoso Evaluation of 99mTc-HYNIC-βAla-Bombesin(7-14) as an agent for pancreas tumor detection in mice Brazilian Journal of Medical and Biological Research 99mTc-HYNIC-βAla-Bombesin Capan-1 Scintigraphic images Pancreas tumor Biodistribution |
author_facet |
F.N. Carlesso L.L. Fuscaldi R.S. Araújo C.S. Teixeira M.C. Oliveira S.O.A. Fernandes G.D. Cassali D.C. Reis A.L.B. Barros V.N. Cardoso |
author_sort |
F.N. Carlesso |
title |
Evaluation of 99mTc-HYNIC-βAla-Bombesin(7-14) as an agent for pancreas tumor detection in mice |
title_short |
Evaluation of 99mTc-HYNIC-βAla-Bombesin(7-14) as an agent for pancreas tumor detection in mice |
title_full |
Evaluation of 99mTc-HYNIC-βAla-Bombesin(7-14) as an agent for pancreas tumor detection in mice |
title_fullStr |
Evaluation of 99mTc-HYNIC-βAla-Bombesin(7-14) as an agent for pancreas tumor detection in mice |
title_full_unstemmed |
Evaluation of 99mTc-HYNIC-βAla-Bombesin(7-14) as an agent for pancreas tumor detection in mice |
title_sort |
evaluation of 99mtc-hynic-βala-bombesin(7-14) as an agent for pancreas tumor detection in mice |
publisher |
Associação Brasileira de Divulgação Científica |
series |
Brazilian Journal of Medical and Biological Research |
issn |
1414-431X |
publishDate |
2015-01-01 |
description |
Pancreatic adenocarcinoma is important in oncology because of its high mortality rate. Deaths may be avoided if an early diagnosis could be achieved. Several types of tumors overexpress gastrin-releasing peptide receptors (GRPr), including pancreatic cancer cells. Thus, a radiolabeled peptide derivative of gastrin-releasing peptide (GRP) may be useful as a specific imaging probe. The purpose of the present study was to evaluate the feasibility of using 99mTc-HYNIC-βAla-Bombesin(7-14) as an imaging probe for Capan-1 pancreatic adenocarcinoma. Xenographic pancreatic tumor was developed in nude mice and characterized by histopathological analysis. Biodistribution studies and scintigraphic images were carried out in tumor-bearing nude mice. The two methods showed higher uptake by pancreatic tumor when compared to muscle (used as control), and the tumor-to-muscle ratio indicated that 99mTc-HYNIC-βAla-Bombesin(7-14) uptake was four-fold higher in tumor cells than in other tissues. Scintigraphic images also showed a clear signal at the tumor site. The present data indicate that 99mTc-HYNIC-βAla-Bombesin(7-14) may be useful for the detection of pancreatic adenocarcinoma. |
topic |
99mTc-HYNIC-βAla-Bombesin Capan-1 Scintigraphic images Pancreas tumor Biodistribution |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015005054506&lng=en&tlng=en |
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