Ferroptosis driven by radical oxidation of n-6 polyunsaturated fatty acids mediates acetaminophen-induced acute liver failure
Abstract Acetaminophen (APAP) overdose is a common cause of drug-induced acute liver failure. Although hepatocyte cell death is considered to be the critical event in APAP-induced hepatotoxicity, the underlying mechanism remains unclear. Ferroptosis is a newly discovered type of cell death that is c...
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2020-02-01
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doaj-4c3e813c18814f5e811902c02c3b77172021-02-23T09:27:21ZengNature Publishing GroupCell Death and Disease2041-48892020-02-0111211610.1038/s41419-020-2334-2Ferroptosis driven by radical oxidation of n-6 polyunsaturated fatty acids mediates acetaminophen-induced acute liver failureNaoya Yamada0Tadayoshi Karasawa1Hiroaki Kimura2Sachiko Watanabe3Takanori Komada4Ryo Kamata5Ariunaa Sampilvanjil6Junya Ito7Kiyotaka Nakagawa8Hiroshi Kuwata9Shuntaro Hara10Koichi Mizuta11Yasunaru Sakuma12Naohiro Sata13Masafumi Takahashi14Division of Inflammation Research, Center for Molecular Medicine, Jichi Medical UniversityDivision of Inflammation Research, Center for Molecular Medicine, Jichi Medical UniversityDivision of Inflammation Research, Center for Molecular Medicine, Jichi Medical UniversityDivision of Inflammation Research, Center for Molecular Medicine, Jichi Medical UniversityDivision of Inflammation Research, Center for Molecular Medicine, Jichi Medical UniversityDivision of Inflammation Research, Center for Molecular Medicine, Jichi Medical UniversityDivision of Inflammation Research, Center for Molecular Medicine, Jichi Medical UniversityFood and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku UniversityFood and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku UniversityDivision of Health Chemistry, Department of Healthcare and Regulatory Sciences, School of Pharmacy, Showa UniversityDivision of Health Chemistry, Department of Healthcare and Regulatory Sciences, School of Pharmacy, Showa UniversityDepartment of Transplant Surgery, Saitama Children’s Medical CenterDivision of Gastroenterological, General and Transplant Surgery, Department of Surgery, Jichi Medical UniversityDivision of Gastroenterological, General and Transplant Surgery, Department of Surgery, Jichi Medical UniversityDivision of Inflammation Research, Center for Molecular Medicine, Jichi Medical UniversityAbstract Acetaminophen (APAP) overdose is a common cause of drug-induced acute liver failure. Although hepatocyte cell death is considered to be the critical event in APAP-induced hepatotoxicity, the underlying mechanism remains unclear. Ferroptosis is a newly discovered type of cell death that is caused by a loss of cellular redox homeostasis. As glutathione (GSH) depletion triggers APAP-induced hepatotoxicity, we investigated the role of ferroptosis in a murine model of APAP-induced acute liver failure. APAP-induced hepatotoxicity (evaluated in terms of ALT, AST, and the histopathological score), lipid peroxidation (4-HNE and MDA), and upregulation of the ferroptosis maker PTGS2 mRNA were markedly prevented by the ferroptosis-specific inhibitor ferrostatin-1 (Fer-1). Fer-1 treatment also completely prevented mortality induced by high-dose APAP. Similarly, APAP-induced hepatotoxicity and lipid peroxidation were prevented by the iron chelator deferoxamine. Using mass spectrometry, we found that lipid peroxides derived from n-6 fatty acids, mainly arachidonic acid, were elevated by APAP, and that auto-oxidation is the predominant mechanism of APAP-derived lipid oxidation. APAP-induced hepatotoxicity was also prevented by genetic inhibition of acyl-CoA synthetase long-chain family member 4 or α-tocopherol supplementation. We found that ferroptosis is responsible for APAP-induced hepatocyte cell death. Our findings provide new insights into the mechanism of APAP-induced hepatotoxicity and suggest that ferroptosis is a potential therapeutic target for APAP-induced acute liver failure.https://doi.org/10.1038/s41419-020-2334-2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Naoya Yamada Tadayoshi Karasawa Hiroaki Kimura Sachiko Watanabe Takanori Komada Ryo Kamata Ariunaa Sampilvanjil Junya Ito Kiyotaka Nakagawa Hiroshi Kuwata Shuntaro Hara Koichi Mizuta Yasunaru Sakuma Naohiro Sata Masafumi Takahashi |
spellingShingle |
Naoya Yamada Tadayoshi Karasawa Hiroaki Kimura Sachiko Watanabe Takanori Komada Ryo Kamata Ariunaa Sampilvanjil Junya Ito Kiyotaka Nakagawa Hiroshi Kuwata Shuntaro Hara Koichi Mizuta Yasunaru Sakuma Naohiro Sata Masafumi Takahashi Ferroptosis driven by radical oxidation of n-6 polyunsaturated fatty acids mediates acetaminophen-induced acute liver failure Cell Death and Disease |
author_facet |
Naoya Yamada Tadayoshi Karasawa Hiroaki Kimura Sachiko Watanabe Takanori Komada Ryo Kamata Ariunaa Sampilvanjil Junya Ito Kiyotaka Nakagawa Hiroshi Kuwata Shuntaro Hara Koichi Mizuta Yasunaru Sakuma Naohiro Sata Masafumi Takahashi |
author_sort |
Naoya Yamada |
title |
Ferroptosis driven by radical oxidation of n-6 polyunsaturated fatty acids mediates acetaminophen-induced acute liver failure |
title_short |
Ferroptosis driven by radical oxidation of n-6 polyunsaturated fatty acids mediates acetaminophen-induced acute liver failure |
title_full |
Ferroptosis driven by radical oxidation of n-6 polyunsaturated fatty acids mediates acetaminophen-induced acute liver failure |
title_fullStr |
Ferroptosis driven by radical oxidation of n-6 polyunsaturated fatty acids mediates acetaminophen-induced acute liver failure |
title_full_unstemmed |
Ferroptosis driven by radical oxidation of n-6 polyunsaturated fatty acids mediates acetaminophen-induced acute liver failure |
title_sort |
ferroptosis driven by radical oxidation of n-6 polyunsaturated fatty acids mediates acetaminophen-induced acute liver failure |
publisher |
Nature Publishing Group |
series |
Cell Death and Disease |
issn |
2041-4889 |
publishDate |
2020-02-01 |
description |
Abstract Acetaminophen (APAP) overdose is a common cause of drug-induced acute liver failure. Although hepatocyte cell death is considered to be the critical event in APAP-induced hepatotoxicity, the underlying mechanism remains unclear. Ferroptosis is a newly discovered type of cell death that is caused by a loss of cellular redox homeostasis. As glutathione (GSH) depletion triggers APAP-induced hepatotoxicity, we investigated the role of ferroptosis in a murine model of APAP-induced acute liver failure. APAP-induced hepatotoxicity (evaluated in terms of ALT, AST, and the histopathological score), lipid peroxidation (4-HNE and MDA), and upregulation of the ferroptosis maker PTGS2 mRNA were markedly prevented by the ferroptosis-specific inhibitor ferrostatin-1 (Fer-1). Fer-1 treatment also completely prevented mortality induced by high-dose APAP. Similarly, APAP-induced hepatotoxicity and lipid peroxidation were prevented by the iron chelator deferoxamine. Using mass spectrometry, we found that lipid peroxides derived from n-6 fatty acids, mainly arachidonic acid, were elevated by APAP, and that auto-oxidation is the predominant mechanism of APAP-derived lipid oxidation. APAP-induced hepatotoxicity was also prevented by genetic inhibition of acyl-CoA synthetase long-chain family member 4 or α-tocopherol supplementation. We found that ferroptosis is responsible for APAP-induced hepatocyte cell death. Our findings provide new insights into the mechanism of APAP-induced hepatotoxicity and suggest that ferroptosis is a potential therapeutic target for APAP-induced acute liver failure. |
url |
https://doi.org/10.1038/s41419-020-2334-2 |
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