Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39
Chitosanases play an important role in chitosan degradation, forming enzymatic degradation products with several biological activities. Although many chitosanases have been discovered and studied, the enzymes with special characteristics are still rather rare. In this study, a new chitosanase, CsnM,...
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doaj-4c44807c81584d0fbef75b9ca5366caa2020-11-25T00:46:11ZengMDPI AGMolecules1420-30492019-01-0124118310.3390/molecules24010183molecules24010183Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39Yu Zhou0Xuehong Chen1Xiao Li2Yantao Han3Yanan Wang4Ruyong Yao5Shangyong Li6Department of Pharmacology, College of Basic Medicine, Qingdao University, Qingdao 266071, ChinaDepartment of Pharmacology, College of Basic Medicine, Qingdao University, Qingdao 266071, ChinaDepartment of Pharmacology, College of Basic Medicine, Qingdao University, Qingdao 266071, ChinaDepartment of Pharmacology, College of Basic Medicine, Qingdao University, Qingdao 266071, ChinaDepartment of Pharmacology, College of Basic Medicine, Qingdao University, Qingdao 266071, ChinaCentral laboratory, Qingdao University, Qingdao 266071, ChinaDepartment of Pharmacology, College of Basic Medicine, Qingdao University, Qingdao 266071, ChinaChitosanases play an important role in chitosan degradation, forming enzymatic degradation products with several biological activities. Although many chitosanases have been discovered and studied, the enzymes with special characteristics are still rather rare. In this study, a new chitosanase, CsnM, with an apparent molecular weight of 28 kDa was purified from the marine bacterium Pseudoalteromonas sp. SY39. CsnM is a cold-adapted enzyme, which shows highest activity at 40 °C and exhibits 30.6% and 49.4% of its maximal activity at 10 and 15 °C, respectively. CsnM is also a thermo-tolerant enzyme that recovers 95.2%, 89.1% and 88.1% of its initial activity after boiling for 5, 10 and 20 min, respectively. Additionally, CsnM is an endo-type chitosanase that yields chitodisaccharide as the main product (69.9% of the total product). It’s cold-adaptation, thermo-tolerance and high chitodisaccharide yield make CsnM a superior candidate for biotechnological application to produce chitooligosaccharides.http://www.mdpi.com/1420-3049/24/1/183chitosanasecold-adaptationthermo-tolerancechitooligosaccharide |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yu Zhou Xuehong Chen Xiao Li Yantao Han Yanan Wang Ruyong Yao Shangyong Li |
spellingShingle |
Yu Zhou Xuehong Chen Xiao Li Yantao Han Yanan Wang Ruyong Yao Shangyong Li Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39 Molecules chitosanase cold-adaptation thermo-tolerance chitooligosaccharide |
author_facet |
Yu Zhou Xuehong Chen Xiao Li Yantao Han Yanan Wang Ruyong Yao Shangyong Li |
author_sort |
Yu Zhou |
title |
Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39 |
title_short |
Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39 |
title_full |
Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39 |
title_fullStr |
Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39 |
title_full_unstemmed |
Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39 |
title_sort |
purification and characterization of a new cold-adapted and thermo-tolerant chitosanase from marine bacterium pseudoalteromonas sp. sy39 |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2019-01-01 |
description |
Chitosanases play an important role in chitosan degradation, forming enzymatic degradation products with several biological activities. Although many chitosanases have been discovered and studied, the enzymes with special characteristics are still rather rare. In this study, a new chitosanase, CsnM, with an apparent molecular weight of 28 kDa was purified from the marine bacterium Pseudoalteromonas sp. SY39. CsnM is a cold-adapted enzyme, which shows highest activity at 40 °C and exhibits 30.6% and 49.4% of its maximal activity at 10 and 15 °C, respectively. CsnM is also a thermo-tolerant enzyme that recovers 95.2%, 89.1% and 88.1% of its initial activity after boiling for 5, 10 and 20 min, respectively. Additionally, CsnM is an endo-type chitosanase that yields chitodisaccharide as the main product (69.9% of the total product). It’s cold-adaptation, thermo-tolerance and high chitodisaccharide yield make CsnM a superior candidate for biotechnological application to produce chitooligosaccharides. |
topic |
chitosanase cold-adaptation thermo-tolerance chitooligosaccharide |
url |
http://www.mdpi.com/1420-3049/24/1/183 |
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