In vitro assessment of potential bladder papillary neoplasm treatment with functionalized polyethyleneimine coated magnetic nanoparticles
Normal porcine urothelial cells have been shown to have a much lower rate of endocytosis than urothelial papillary neoplasm cells. This could be used as a mechanism for selective delivery of toxic compounds, such as polyethyleneimine coated nanoparticles (NPs). However, these NPs induce nonselective...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Slovenian Chemical Society
2017-09-01
|
Series: | Acta Chimica Slovenica |
Subjects: | |
Online Access: | https://journals.matheo.si/index.php/ACSi/article/view/2876 |
id |
doaj-4c4dfaea88cd40328983b5b5b1cb5a31 |
---|---|
record_format |
Article |
spelling |
doaj-4c4dfaea88cd40328983b5b5b1cb5a312020-11-25T01:28:54ZengSlovenian Chemical SocietyActa Chimica Slovenica1318-02071580-31552017-09-0164354354810.17344/acsi.2016.2876450In vitro assessment of potential bladder papillary neoplasm treatment with functionalized polyethyleneimine coated magnetic nanoparticlesKlemen Strojan0Jasna Lojk1Vladimir Boštjan Bregar2Mateja Erdani Kreft3Jurij Svete4Peter Veranič5Mojca Pavlin6Group for nano and biotechnological applications, University of Ljubljana, Faculty of Electrical Engineering, Trzaska cesta 25, Ljubljana, SloveniaGroup for nano and biotechnological applications, University of Ljubljana, Faculty of Electrical Engineering, Trzaska cesta 25, Ljubljana, Slovenia Institute of Cell biology, University of Ljubljana, Faculty of Medicine, Vrazov trg 2, Ljubljana, SloveniaGroup for nano and biotechnological applications, University of Ljubljana, Faculty of Electrical Engineering, Trzaska cesta 25, Ljubljana, SloveniaInstitute of Cell biology, University of Ljubljana, Faculty of Medicine, Vrazov trg 2, Ljubljana, SloveniaFaculty of Chemistry and Chemical Technology, Vecna Pot 113, Ljubljana, SloveniaInstitute of Cell biology, University of Ljubljana, Faculty of Medicine, Vrazov trg 2, Ljubljana, SloveniaGroup for nano and biotechnological applications, University of Ljubljana, Faculty of Electrical Engineering, Trzaska cesta 25, Ljubljana, Slovenia Institute of Biophysics, University of Ljubljana, Faculty of Medicine, Vrazov trg 2, Ljubljana, SloveniaNormal porcine urothelial cells have been shown to have a much lower rate of endocytosis than urothelial papillary neoplasm cells. This could be used as a mechanism for selective delivery of toxic compounds, such as polyethyleneimine coated nanoparticles (NPs). However, these NPs induce nonselective toxicity through direct membrane disruption. This toxicity can be reduced by functionalization of NPs with L-glutathione reduced or bovine serum albumin by reducing their surface charge. Functionalization was confirmed with Fourier Transform Infrared Spectroscopy, Dynamic Light Scattering and Zeta potential measurements. Viability assays showed that bovine serum albumin coating reduced NPs cytotoxicity immediately after 3 h exposure and that such NPs were more toxic to urothelial papillary neoplasm cells compared to normal porcine urothelial cells at 50 µg/ml NPs concentration. However, 24 h after exposure, bovine serum albumin functionalized NPs had similar effect on viability of both cell lines. NPs showed some selective toxicity towards urothelial papillary neoplasm cells compared to normal cells after 3 h, however this was not confirmed after 24 h.https://journals.matheo.si/index.php/ACSi/article/view/2876polyethyleneimineurothelial cell modelsmagnetic nanoparticlestoxicityurothelial papillary neoplasm |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Klemen Strojan Jasna Lojk Vladimir Boštjan Bregar Mateja Erdani Kreft Jurij Svete Peter Veranič Mojca Pavlin |
spellingShingle |
Klemen Strojan Jasna Lojk Vladimir Boštjan Bregar Mateja Erdani Kreft Jurij Svete Peter Veranič Mojca Pavlin In vitro assessment of potential bladder papillary neoplasm treatment with functionalized polyethyleneimine coated magnetic nanoparticles Acta Chimica Slovenica polyethyleneimine urothelial cell models magnetic nanoparticles toxicity urothelial papillary neoplasm |
author_facet |
Klemen Strojan Jasna Lojk Vladimir Boštjan Bregar Mateja Erdani Kreft Jurij Svete Peter Veranič Mojca Pavlin |
author_sort |
Klemen Strojan |
title |
In vitro assessment of potential bladder papillary neoplasm treatment with functionalized polyethyleneimine coated magnetic nanoparticles |
title_short |
In vitro assessment of potential bladder papillary neoplasm treatment with functionalized polyethyleneimine coated magnetic nanoparticles |
title_full |
In vitro assessment of potential bladder papillary neoplasm treatment with functionalized polyethyleneimine coated magnetic nanoparticles |
title_fullStr |
In vitro assessment of potential bladder papillary neoplasm treatment with functionalized polyethyleneimine coated magnetic nanoparticles |
title_full_unstemmed |
In vitro assessment of potential bladder papillary neoplasm treatment with functionalized polyethyleneimine coated magnetic nanoparticles |
title_sort |
in vitro assessment of potential bladder papillary neoplasm treatment with functionalized polyethyleneimine coated magnetic nanoparticles |
publisher |
Slovenian Chemical Society |
series |
Acta Chimica Slovenica |
issn |
1318-0207 1580-3155 |
publishDate |
2017-09-01 |
description |
Normal porcine urothelial cells have been shown to have a much lower rate of endocytosis than urothelial papillary neoplasm cells. This could be used as a mechanism for selective delivery of toxic compounds, such as polyethyleneimine coated nanoparticles (NPs). However, these NPs induce nonselective toxicity through direct membrane disruption. This toxicity can be reduced by functionalization of NPs with L-glutathione reduced or bovine serum albumin by reducing their surface charge. Functionalization was confirmed with Fourier Transform Infrared Spectroscopy, Dynamic Light Scattering and Zeta potential measurements. Viability assays showed that bovine serum albumin coating reduced NPs cytotoxicity immediately after 3 h exposure and that such NPs were more toxic to urothelial papillary neoplasm cells compared to normal porcine urothelial cells at 50 µg/ml NPs concentration. However, 24 h after exposure, bovine serum albumin functionalized NPs had similar effect on viability of both cell lines. NPs showed some selective toxicity towards urothelial papillary neoplasm cells compared to normal cells after 3 h, however this was not confirmed after 24 h. |
topic |
polyethyleneimine urothelial cell models magnetic nanoparticles toxicity urothelial papillary neoplasm |
url |
https://journals.matheo.si/index.php/ACSi/article/view/2876 |
work_keys_str_mv |
AT klemenstrojan invitroassessmentofpotentialbladderpapillaryneoplasmtreatmentwithfunctionalizedpolyethyleneiminecoatedmagneticnanoparticles AT jasnalojk invitroassessmentofpotentialbladderpapillaryneoplasmtreatmentwithfunctionalizedpolyethyleneiminecoatedmagneticnanoparticles AT vladimirbostjanbregar invitroassessmentofpotentialbladderpapillaryneoplasmtreatmentwithfunctionalizedpolyethyleneiminecoatedmagneticnanoparticles AT matejaerdanikreft invitroassessmentofpotentialbladderpapillaryneoplasmtreatmentwithfunctionalizedpolyethyleneiminecoatedmagneticnanoparticles AT jurijsvete invitroassessmentofpotentialbladderpapillaryneoplasmtreatmentwithfunctionalizedpolyethyleneiminecoatedmagneticnanoparticles AT peterveranic invitroassessmentofpotentialbladderpapillaryneoplasmtreatmentwithfunctionalizedpolyethyleneiminecoatedmagneticnanoparticles AT mojcapavlin invitroassessmentofpotentialbladderpapillaryneoplasmtreatmentwithfunctionalizedpolyethyleneiminecoatedmagneticnanoparticles |
_version_ |
1725099597070598144 |