Isolation Of Compounds Of Steroids Teripang Gamat (Stichopus variegatus) With Various Types Of Solvents
Sea cucumber is one of the fisheries commodity that has an important economic value. Generally is<br />traded in dried form (beche-de-mer). One of thebioactive substances contained in sea cucumber is steroid<br />compounds that serves as an aphrodisiac and sex reversal. The purpose of th...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Bogor Agricultural University
2016-12-01
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Series: | Jurnal Pengolahan Hasil Perikanan Indonesia |
Subjects: | |
Online Access: | http://jurnal.ipb.ac.id/index.php/jphpi/article/view/14548 |
Summary: | Sea cucumber is one of the fisheries commodity that has an important economic value. Generally is<br />traded in dried form (beche-de-mer). One of thebioactive substances contained in sea cucumber is steroid<br />compounds that serves as an aphrodisiac and sex reversal. The purpose of this study was to extract the<br />steroid of the gamma sea cucumber by using three types of solvents (methanol, ethyl acetate and hexane)<br />and get the best solvent in producing the highest yield of the steroids. The study revealed that steroid of<br />gamma sea cucumber (Stichopus variegatus) dissolved completely ethyl acetate (semi-polar solvent) during<br />the first phase, second phase and the third phase of extraction. In the methanol (polar solvent) steroids only<br />dissolved in the first extraction phase, while using the hexane (non polar solvent) steroid was undetectable.<br />Fractionation by thin layer chromatography was obtained two fractions that identified as cholesterol (Rf =<br />0.96) and testosterone (Rf = 0.91).<br /><br /> |
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ISSN: | 2303-2111 2354-886X |