MKL-1 regulates the stem cell marker. CD44 in breast cancer cells

• Main Authors: Dai Zhou-Tong, Yao Ao, Xiang Yuan, Li Jia Peng, Guo Wei, Zhang Hui-Min, Huang Feng, Li Han-Han, Zhang Zi-Jiang, Li Hui, Liao Xing Hua
• Format: Article
• Language: English
• Published: EDP Sciences 2019-01-01
• Series: E3S Web of Conferences
• Online Access: https://doi.org/10.1051/e3sconf/20197801002

CD44, cluster of differentiation 44 is a typical marker of stem cells. At present, it has been found that CD44 is prevalent in various human malignant tumors, but its expression regulation mechanism is still not clear. The initiation of gene expression, the modification of RNA levels, and the regulation of protein levels are the main factors affecting the expression level of genes, and the most critical one is the regulation of gene expression by signaling pathways. Up to now, there has been no report on the role of MKL-1 in the cloning of the cd44 promoter. Therefore, this study intends to clone the cd44 gene promoter, construct its luciferase reporter gene vector, transfect the MKL-1 overexpression vector, and analyze how it affects transcriptional activity, in order to further study the expression regulation of cd44. The mechanism provides a powerful tool in the future.