Blood Transcriptomics of Turbot <i>Scophthalmus maximus</i>: A Tool for Health Monitoring and Disease Studies
Blood transcriptomics is emerging as a relevant tool to monitor the status of the immune system and assist in diagnosis, prognosis, treatment and pathogenesis studies of diseases. In fish pathology, the potential of transcriptome profiling of blood is still poorly explored. Here, RNA sequencing was...
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doaj-4c6c2e3674054442bd2ccfb812d7abc42021-04-30T23:05:56ZengMDPI AGAnimals2076-26152021-04-01111296129610.3390/ani11051296Blood Transcriptomics of Turbot <i>Scophthalmus maximus</i>: A Tool for Health Monitoring and Disease StudiesPaolo Ronza0José Antonio Álvarez-Dios1Diego Robledo2Ana Paula Losada3Roberto Romero4Roberto Bermúdez5Belén G. Pardo6Paulino Martínez7María Isabel Quiroga8Departamento de Anatomía, Producción Animal y Ciencias Clínicas Veterinarias, Facultad de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, SpainDepartamento de Matemática Aplicada, Facultad de Matemáticas, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, SpainThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian EH25 9RG, UKDepartamento de Anatomía, Producción Animal y Ciencias Clínicas Veterinarias, Facultad de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, SpainInsuiña SL, Playa del Lago, 27870 Xove, Lugo, SpainDepartamento de Anatomía, Producción Animal y Ciencias Clínicas Veterinarias, Facultad de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, SpainInstituto de Acuicultura, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, SpainInstituto de Acuicultura, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, SpainDepartamento de Anatomía, Producción Animal y Ciencias Clínicas Veterinarias, Facultad de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, SpainBlood transcriptomics is emerging as a relevant tool to monitor the status of the immune system and assist in diagnosis, prognosis, treatment and pathogenesis studies of diseases. In fish pathology, the potential of transcriptome profiling of blood is still poorly explored. Here, RNA sequencing was applied to analyze the blood transcriptional profile of turbot (<i>Scophthalmus maximus</i>), the most important farmed flatfish. The study was conducted in healthy specimens and specimens parasitized by the myxozoan <i>Enteromyxum scophthalmi</i>, which causes one of the most devastating diseases in turbot aquaculture. The blood of healthy turbot showed a transcriptomic profile mainly related to erythrocyte gas transportation function, but also to antigen processing and presentation. In moderately infected turbot, the blood reflected a broad inhibition of the immune response. Particularly, down-regulation of the B cell receptor signaling pathway was shared with heavily parasitized fish, which showed larger transcriptomic changes, including the activation of the inflammatory response. Turbot response to enteromyxosis proved to be delayed, dysregulated and ineffective in stopping the infection. The study evinces that blood transcriptomics can contribute to a better understanding of the teleost immune system and serve as a reliable tool to investigate the physiopathological status of fish.https://www.mdpi.com/2076-2615/11/5/1296RNA-seqteleostimmune systemimmune responseerythrocytesleukocytes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Paolo Ronza José Antonio Álvarez-Dios Diego Robledo Ana Paula Losada Roberto Romero Roberto Bermúdez Belén G. Pardo Paulino Martínez María Isabel Quiroga |
spellingShingle |
Paolo Ronza José Antonio Álvarez-Dios Diego Robledo Ana Paula Losada Roberto Romero Roberto Bermúdez Belén G. Pardo Paulino Martínez María Isabel Quiroga Blood Transcriptomics of Turbot <i>Scophthalmus maximus</i>: A Tool for Health Monitoring and Disease Studies Animals RNA-seq teleost immune system immune response erythrocytes leukocytes |
author_facet |
Paolo Ronza José Antonio Álvarez-Dios Diego Robledo Ana Paula Losada Roberto Romero Roberto Bermúdez Belén G. Pardo Paulino Martínez María Isabel Quiroga |
author_sort |
Paolo Ronza |
title |
Blood Transcriptomics of Turbot <i>Scophthalmus maximus</i>: A Tool for Health Monitoring and Disease Studies |
title_short |
Blood Transcriptomics of Turbot <i>Scophthalmus maximus</i>: A Tool for Health Monitoring and Disease Studies |
title_full |
Blood Transcriptomics of Turbot <i>Scophthalmus maximus</i>: A Tool for Health Monitoring and Disease Studies |
title_fullStr |
Blood Transcriptomics of Turbot <i>Scophthalmus maximus</i>: A Tool for Health Monitoring and Disease Studies |
title_full_unstemmed |
Blood Transcriptomics of Turbot <i>Scophthalmus maximus</i>: A Tool for Health Monitoring and Disease Studies |
title_sort |
blood transcriptomics of turbot <i>scophthalmus maximus</i>: a tool for health monitoring and disease studies |
publisher |
MDPI AG |
series |
Animals |
issn |
2076-2615 |
publishDate |
2021-04-01 |
description |
Blood transcriptomics is emerging as a relevant tool to monitor the status of the immune system and assist in diagnosis, prognosis, treatment and pathogenesis studies of diseases. In fish pathology, the potential of transcriptome profiling of blood is still poorly explored. Here, RNA sequencing was applied to analyze the blood transcriptional profile of turbot (<i>Scophthalmus maximus</i>), the most important farmed flatfish. The study was conducted in healthy specimens and specimens parasitized by the myxozoan <i>Enteromyxum scophthalmi</i>, which causes one of the most devastating diseases in turbot aquaculture. The blood of healthy turbot showed a transcriptomic profile mainly related to erythrocyte gas transportation function, but also to antigen processing and presentation. In moderately infected turbot, the blood reflected a broad inhibition of the immune response. Particularly, down-regulation of the B cell receptor signaling pathway was shared with heavily parasitized fish, which showed larger transcriptomic changes, including the activation of the inflammatory response. Turbot response to enteromyxosis proved to be delayed, dysregulated and ineffective in stopping the infection. The study evinces that blood transcriptomics can contribute to a better understanding of the teleost immune system and serve as a reliable tool to investigate the physiopathological status of fish. |
topic |
RNA-seq teleost immune system immune response erythrocytes leukocytes |
url |
https://www.mdpi.com/2076-2615/11/5/1296 |
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