Silver Nanoparticles on Chitosan/Silica Nanofibers: Characterization and Antibacterial Activity
A simple, low-cost, and reproducible method for creating materials with even silver nanoparticles (AgNP) dispersion was established. Chitosan nanofibers with silica phase (CS/silica) were synthesized by an electrospinning technique to obtain highly porous 3D nanofiber scaffolds. Silver nanoparticles...
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doaj-4c89fcc5a870465280cf51c3208d6cd32020-11-25T00:13:22ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-12-0121116610.3390/ijms21010166ijms21010166Silver Nanoparticles on Chitosan/Silica Nanofibers: Characterization and Antibacterial ActivityMałgorzata Zienkiewicz-Strzałka0Anna Deryło-Marczewska1Yury A. Skorik2Valentina A. Petrova3Adam Choma4Iwona Komaniecka5Department of Physical Chemistry, Institute of Chemical Sciences, Maria Curie-Skłodowska University, sq. Maria Curie-Skłodowska 3, 20-031 Lublin, PolandDepartment of Physical Chemistry, Institute of Chemical Sciences, Maria Curie-Skłodowska University, sq. Maria Curie-Skłodowska 3, 20-031 Lublin, PolandInstitute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoi pr. VO 31, St. Petersburg 199004, RussiaInstitute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoi pr. VO 31, St. Petersburg 199004, RussiaDepartment of Genetics and Microbiology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, PolandDepartment of Genetics and Microbiology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, PolandA simple, low-cost, and reproducible method for creating materials with even silver nanoparticles (AgNP) dispersion was established. Chitosan nanofibers with silica phase (CS/silica) were synthesized by an electrospinning technique to obtain highly porous 3D nanofiber scaffolds. Silver nanoparticles in the form of a well-dispersed metallic phase were synthesized in an external preparation step and embedded in the CS/silica nanofibers by deposition for obtaining chitosan nanofibers with silica phase decorated by silver nanoparticles (Ag/CS/silica). The antibacterial activity of investigated materials was tested using Gram-positive and Gram-negative bacteria. The results were compared with the properties of the nanocomposite without silver nanoparticles and a colloidal solution of AgNP. The minimum inhibitory concentration (MIC) of obtained AgNP against <i>Staphylococcus aureus</i> (<i>S. aureus</i>) ATCC25923 and <i>Escherichia coli</i> (<i>E. coli</i>) ATCC25922 was determined. The physicochemical characterization of Ag/CS/silica nanofibers using various analytical techniques, as well as the applicability of these techniques in the characterization of this type of nanocomposite, is presented. The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM). The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).https://www.mdpi.com/1422-0067/21/1/166chitosan nanofibernanocompositesilver nanoparticlesantibacterial activityelectrospinning |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Małgorzata Zienkiewicz-Strzałka Anna Deryło-Marczewska Yury A. Skorik Valentina A. Petrova Adam Choma Iwona Komaniecka |
spellingShingle |
Małgorzata Zienkiewicz-Strzałka Anna Deryło-Marczewska Yury A. Skorik Valentina A. Petrova Adam Choma Iwona Komaniecka Silver Nanoparticles on Chitosan/Silica Nanofibers: Characterization and Antibacterial Activity International Journal of Molecular Sciences chitosan nanofiber nanocomposite silver nanoparticles antibacterial activity electrospinning |
author_facet |
Małgorzata Zienkiewicz-Strzałka Anna Deryło-Marczewska Yury A. Skorik Valentina A. Petrova Adam Choma Iwona Komaniecka |
author_sort |
Małgorzata Zienkiewicz-Strzałka |
title |
Silver Nanoparticles on Chitosan/Silica Nanofibers: Characterization and Antibacterial Activity |
title_short |
Silver Nanoparticles on Chitosan/Silica Nanofibers: Characterization and Antibacterial Activity |
title_full |
Silver Nanoparticles on Chitosan/Silica Nanofibers: Characterization and Antibacterial Activity |
title_fullStr |
Silver Nanoparticles on Chitosan/Silica Nanofibers: Characterization and Antibacterial Activity |
title_full_unstemmed |
Silver Nanoparticles on Chitosan/Silica Nanofibers: Characterization and Antibacterial Activity |
title_sort |
silver nanoparticles on chitosan/silica nanofibers: characterization and antibacterial activity |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-12-01 |
description |
A simple, low-cost, and reproducible method for creating materials with even silver nanoparticles (AgNP) dispersion was established. Chitosan nanofibers with silica phase (CS/silica) were synthesized by an electrospinning technique to obtain highly porous 3D nanofiber scaffolds. Silver nanoparticles in the form of a well-dispersed metallic phase were synthesized in an external preparation step and embedded in the CS/silica nanofibers by deposition for obtaining chitosan nanofibers with silica phase decorated by silver nanoparticles (Ag/CS/silica). The antibacterial activity of investigated materials was tested using Gram-positive and Gram-negative bacteria. The results were compared with the properties of the nanocomposite without silver nanoparticles and a colloidal solution of AgNP. The minimum inhibitory concentration (MIC) of obtained AgNP against <i>Staphylococcus aureus</i> (<i>S. aureus</i>) ATCC25923 and <i>Escherichia coli</i> (<i>E. coli</i>) ATCC25922 was determined. The physicochemical characterization of Ag/CS/silica nanofibers using various analytical techniques, as well as the applicability of these techniques in the characterization of this type of nanocomposite, is presented. The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM). The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM). |
topic |
chitosan nanofiber nanocomposite silver nanoparticles antibacterial activity electrospinning |
url |
https://www.mdpi.com/1422-0067/21/1/166 |
work_keys_str_mv |
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