Magnetic resonance imaging investigations reveal that PM2.5 exposure triggers visual dysfunction in mice
Objectives: To investigate how PM2.5 exposure affects the microstructure, metabolites or functions of the visual system. Methods: C57BL/6J mice were randomly assigned to groups exposed to the filtered air (the control group) or the concentrated ambient PM2.5 (the PM2.5 group). Visual evoked potentia...
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Elsevier
2021-12-01
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Series: | Ecotoxicology and Environmental Safety |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651321009787 |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Linying Guo He Wang Ji Zhou Weijun Tang Rong Wang Zebin Xiao Lingjie Wu Jie Wang Liping Li Yuan Lei Xinghuai Sun Zuohua Tang |
spellingShingle |
Linying Guo He Wang Ji Zhou Weijun Tang Rong Wang Zebin Xiao Lingjie Wu Jie Wang Liping Li Yuan Lei Xinghuai Sun Zuohua Tang Magnetic resonance imaging investigations reveal that PM2.5 exposure triggers visual dysfunction in mice Ecotoxicology and Environmental Safety Diffusion tensor imaging PM2.5 exposure Proton magnetic resonance spectroscopy Resting state functional MRI Visual electrophysiology |
author_facet |
Linying Guo He Wang Ji Zhou Weijun Tang Rong Wang Zebin Xiao Lingjie Wu Jie Wang Liping Li Yuan Lei Xinghuai Sun Zuohua Tang |
author_sort |
Linying Guo |
title |
Magnetic resonance imaging investigations reveal that PM2.5 exposure triggers visual dysfunction in mice |
title_short |
Magnetic resonance imaging investigations reveal that PM2.5 exposure triggers visual dysfunction in mice |
title_full |
Magnetic resonance imaging investigations reveal that PM2.5 exposure triggers visual dysfunction in mice |
title_fullStr |
Magnetic resonance imaging investigations reveal that PM2.5 exposure triggers visual dysfunction in mice |
title_full_unstemmed |
Magnetic resonance imaging investigations reveal that PM2.5 exposure triggers visual dysfunction in mice |
title_sort |
magnetic resonance imaging investigations reveal that pm2.5 exposure triggers visual dysfunction in mice |
publisher |
Elsevier |
series |
Ecotoxicology and Environmental Safety |
issn |
0147-6513 |
publishDate |
2021-12-01 |
description |
Objectives: To investigate how PM2.5 exposure affects the microstructure, metabolites or functions of the visual system. Methods: C57BL/6J mice were randomly assigned to groups exposed to the filtered air (the control group) or the concentrated ambient PM2.5 (the PM2.5 group). Visual evoked potentials (VEP), electroretinograms (ERG), diffusion tensor imaging (DTI), proton magnetic resonance spectroscopy (1H-MRS) and resting-state functional MRI (rsfMRI) were performed. Parameters were obtained and compared between the two groups, including latencies and amplitudes of the P1 wave, N1 wave and P2 wave from VEP, latencies and amplitudes of the a wave and b wave from ERG, fractional anisotropy (FA), mean diffusion (MD), axial diffusivity (AD) and radial diffusivity (RD) from DTI, visual cortex (VC) metabolites from 1H-MRS, and regional homogeneity (ReHo) from rsfMRI. Results: Compared with the values of the control group, the PM2.5 group showed a prolonged N1 latency (43.11 ± 7.94 ms vs. 38.75 ± 4.60 ms) and lowered P1 amplitude (5.62 ± 4.38 μV vs. 8.56 ± 5.92 μV) on VEP (all p < 0.05). On ERG, the amplitude of the a wave was lowered (− 91.39 ± 56.29 μV vs. − 138.68 ± 89.05 μV), the amplitude of the b wave was lowered (194.38 ± 126.27 μV vs. 284.72 ± 170.99 μV), and the latency of the b wave was prolonged (37.78 ± 10.72 ms vs. 33.01 ± 4.34 ms) than the values of the control group (all p < 0.05). DTI indicated FA increase in the bilateral piriform cortex (Pir), FA decrease in the bilateral somatosensory cortex (S) and the bilateral striatum (Stri), AD decrease in the bilateral VC, the right S and the bilateral Pir, MD decrease in the bilateral Pir, and RD decrease in the bilateral Pir in the PM2.5 mice (all p < 0.05, Alphasim corrected). 1H-MRS showed Glutamate (Glu) increase and Phosphocholine (PCh) increase in the VC of the PM2.5 group than those of the control group (PCh 1.63 ± 0.25 vs. 1.50 ± 0.25; PCh/total creatine(tCr) 0.19 ± 0.03 vs. 0.18 ± 0.03; Glu 10.46 ± 1.50 vs. 9.60 ± 1.19; Glu/tcr 1.23 ± 0.11 vs. 1.12 ± 0.11) (all p < 0.05). rsfMRI showed higher ReHo in the PM2.5 mice in the left superior colliculus, the left motor cortex, the hippocampus, the periaqueductal gray and the right mesencephalic reticular formation (all p < 0.01, AlphaSim corrected). Conclusions: This study revealed that PM2.5 exposure triggered visual dysfunction, and altered microstructure, metabolite and function in the retina and visual brain areas along the visual system. |
topic |
Diffusion tensor imaging PM2.5 exposure Proton magnetic resonance spectroscopy Resting state functional MRI Visual electrophysiology |
url |
http://www.sciencedirect.com/science/article/pii/S0147651321009787 |
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doaj-4c8e2814a6514118ac9ab6aeb88d08852021-10-09T04:35:20ZengElsevierEcotoxicology and Environmental Safety0147-65132021-12-01227112866Magnetic resonance imaging investigations reveal that PM2.5 exposure triggers visual dysfunction in miceLinying Guo0He Wang1Ji Zhou2Weijun Tang3Rong Wang4Zebin Xiao5Lingjie Wu6Jie Wang7Liping Li8Yuan Lei9Xinghuai Sun10Zuohua Tang11Department of Radiology, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, ChinaInstitute of Science and Technology for Brain-Inspired Intelligence, Fudan University, ChinaShanghai Key Laboratory of Meteorology and Health, Shanghai Meteorological Bureau, Shanghai 200030, China; Shanghai Typhoon Institute, CMA, Shanghai 200030, China; Department of Atmospheric and Oceanic Sciences & Institute of Atmospheric Sciences, Fudan University, Shanghai 200031, ChinaDepartment of Radiology, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, ChinaDepartment of Radiology, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, ChinaDepartment of Radiology, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China; Department of Biomedical Sciences, University of Pennsylvania, 22, Philadelphia, PA 19104, United StatesDepartment of Ear, Nose & Throat, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, ChinaDepartment of Radiotherapy, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, ChinaDepartment of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, ChinaDepartment of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China; NHC Key Laboratory of Myopia, Chinese Academy of Medical Sciences, and Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, China; Correspondence to: Department of Ophthalmology & Visual Science, Eye Institute, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China.Department of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China; NHC Key Laboratory of Myopia, Chinese Academy of Medical Sciences, and Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, China; State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai 200032, China; Corresponding author at: Department of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China.Department of Radiology, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China; Correspondence to: Department of Radiology, Eye & ENT Hospital, Shanghai Medical College, Fudan University, 83 Fenyang Road, Shanghai 200031, China.Objectives: To investigate how PM2.5 exposure affects the microstructure, metabolites or functions of the visual system. Methods: C57BL/6J mice were randomly assigned to groups exposed to the filtered air (the control group) or the concentrated ambient PM2.5 (the PM2.5 group). Visual evoked potentials (VEP), electroretinograms (ERG), diffusion tensor imaging (DTI), proton magnetic resonance spectroscopy (1H-MRS) and resting-state functional MRI (rsfMRI) were performed. Parameters were obtained and compared between the two groups, including latencies and amplitudes of the P1 wave, N1 wave and P2 wave from VEP, latencies and amplitudes of the a wave and b wave from ERG, fractional anisotropy (FA), mean diffusion (MD), axial diffusivity (AD) and radial diffusivity (RD) from DTI, visual cortex (VC) metabolites from 1H-MRS, and regional homogeneity (ReHo) from rsfMRI. Results: Compared with the values of the control group, the PM2.5 group showed a prolonged N1 latency (43.11 ± 7.94 ms vs. 38.75 ± 4.60 ms) and lowered P1 amplitude (5.62 ± 4.38 μV vs. 8.56 ± 5.92 μV) on VEP (all p < 0.05). On ERG, the amplitude of the a wave was lowered (− 91.39 ± 56.29 μV vs. − 138.68 ± 89.05 μV), the amplitude of the b wave was lowered (194.38 ± 126.27 μV vs. 284.72 ± 170.99 μV), and the latency of the b wave was prolonged (37.78 ± 10.72 ms vs. 33.01 ± 4.34 ms) than the values of the control group (all p < 0.05). DTI indicated FA increase in the bilateral piriform cortex (Pir), FA decrease in the bilateral somatosensory cortex (S) and the bilateral striatum (Stri), AD decrease in the bilateral VC, the right S and the bilateral Pir, MD decrease in the bilateral Pir, and RD decrease in the bilateral Pir in the PM2.5 mice (all p < 0.05, Alphasim corrected). 1H-MRS showed Glutamate (Glu) increase and Phosphocholine (PCh) increase in the VC of the PM2.5 group than those of the control group (PCh 1.63 ± 0.25 vs. 1.50 ± 0.25; PCh/total creatine(tCr) 0.19 ± 0.03 vs. 0.18 ± 0.03; Glu 10.46 ± 1.50 vs. 9.60 ± 1.19; Glu/tcr 1.23 ± 0.11 vs. 1.12 ± 0.11) (all p < 0.05). rsfMRI showed higher ReHo in the PM2.5 mice in the left superior colliculus, the left motor cortex, the hippocampus, the periaqueductal gray and the right mesencephalic reticular formation (all p < 0.01, AlphaSim corrected). Conclusions: This study revealed that PM2.5 exposure triggered visual dysfunction, and altered microstructure, metabolite and function in the retina and visual brain areas along the visual system.http://www.sciencedirect.com/science/article/pii/S0147651321009787Diffusion tensor imagingPM2.5 exposureProton magnetic resonance spectroscopyResting state functional MRIVisual electrophysiology |