RNASeq Analysis of <i>Aedes albopictus</i> Mosquito Midguts after Chikungunya Virus Infection
Chikungunya virus (CHIKV) is an emerging pathogen around the world and causes significant morbidity in patients. A single amino acid mutation in the envelope protein of CHIKV has led to a shift in vector preference towards <i>Aedes</i> <i>albopictus</i>. While mosquitoes are...
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doaj-4c960e5d80184b84922894d28763635e2020-11-25T01:11:34ZengMDPI AGViruses1999-49152019-06-0111651310.3390/v11060513v11060513RNASeq Analysis of <i>Aedes albopictus</i> Mosquito Midguts after Chikungunya Virus InfectionRavi kiran Vedururu0Matthew J. Neave1Mary Tachedjian2Melissa J. Klein3Paul R. Gorry4Jean-Bernard Duchemin5Prasad N. Paradkar6CSIRO Health & Biosecurity, Australian Animal Health Laboratory, Geelong 3220, AustraliaCSIRO Health & Biosecurity, Australian Animal Health Laboratory, Geelong 3220, AustraliaCSIRO Health & Biosecurity, Australian Animal Health Laboratory, Geelong 3220, AustraliaCSIRO Health & Biosecurity, Australian Animal Health Laboratory, Geelong 3220, AustraliaSchool of Health and Biomedical Science, RMIT University, Bundoora 3083, AustraliaCSIRO Health & Biosecurity, Australian Animal Health Laboratory, Geelong 3220, AustraliaCSIRO Health & Biosecurity, Australian Animal Health Laboratory, Geelong 3220, AustraliaChikungunya virus (CHIKV) is an emerging pathogen around the world and causes significant morbidity in patients. A single amino acid mutation in the envelope protein of CHIKV has led to a shift in vector preference towards <i>Aedes</i> <i>albopictus</i>. While mosquitoes are known to mount an antiviral immune response post-infection, molecular interactions during the course of infection at the tissue level remain largely uncharacterised. We performed whole transcriptome analysis on dissected midguts of <i>Aedes albopictus</i> infected with CHIKV to identify differentially expressed genes. For this, RNA was extracted at two days post-infection (2-dpi) from pooled midguts. We initially identified 25 differentially expressed genes (<i>p</i>-value < 0.05) when mapped to a reference transcriptome. Further, multiple differentially expressed genes were identified from a custom <i>de novo</i> transcriptome, which was assembled using the reads that did not align with the reference genome. Thirteen of the identified transcripts, possibly involved in immunity, were validated by qRT-PCR. Homologues of seven of these genes were also found to be significantly upregulated in <i>Aedes aegypti</i> midguts 2 dpi, indicating a conserved mechanism at play. These results will help us to characterise the molecular interaction between <i>Aedes albopictus</i> and CHIKV and can be utilised to reduce the impact of this viral infection.https://www.mdpi.com/1999-4915/11/6/513Chikungunya<i>Aedes albopictus</i>RNASeqHost–pathogen interactions |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ravi kiran Vedururu Matthew J. Neave Mary Tachedjian Melissa J. Klein Paul R. Gorry Jean-Bernard Duchemin Prasad N. Paradkar |
spellingShingle |
Ravi kiran Vedururu Matthew J. Neave Mary Tachedjian Melissa J. Klein Paul R. Gorry Jean-Bernard Duchemin Prasad N. Paradkar RNASeq Analysis of <i>Aedes albopictus</i> Mosquito Midguts after Chikungunya Virus Infection Viruses Chikungunya <i>Aedes albopictus</i> RNASeq Host–pathogen interactions |
author_facet |
Ravi kiran Vedururu Matthew J. Neave Mary Tachedjian Melissa J. Klein Paul R. Gorry Jean-Bernard Duchemin Prasad N. Paradkar |
author_sort |
Ravi kiran Vedururu |
title |
RNASeq Analysis of <i>Aedes albopictus</i> Mosquito Midguts after Chikungunya Virus Infection |
title_short |
RNASeq Analysis of <i>Aedes albopictus</i> Mosquito Midguts after Chikungunya Virus Infection |
title_full |
RNASeq Analysis of <i>Aedes albopictus</i> Mosquito Midguts after Chikungunya Virus Infection |
title_fullStr |
RNASeq Analysis of <i>Aedes albopictus</i> Mosquito Midguts after Chikungunya Virus Infection |
title_full_unstemmed |
RNASeq Analysis of <i>Aedes albopictus</i> Mosquito Midguts after Chikungunya Virus Infection |
title_sort |
rnaseq analysis of <i>aedes albopictus</i> mosquito midguts after chikungunya virus infection |
publisher |
MDPI AG |
series |
Viruses |
issn |
1999-4915 |
publishDate |
2019-06-01 |
description |
Chikungunya virus (CHIKV) is an emerging pathogen around the world and causes significant morbidity in patients. A single amino acid mutation in the envelope protein of CHIKV has led to a shift in vector preference towards <i>Aedes</i> <i>albopictus</i>. While mosquitoes are known to mount an antiviral immune response post-infection, molecular interactions during the course of infection at the tissue level remain largely uncharacterised. We performed whole transcriptome analysis on dissected midguts of <i>Aedes albopictus</i> infected with CHIKV to identify differentially expressed genes. For this, RNA was extracted at two days post-infection (2-dpi) from pooled midguts. We initially identified 25 differentially expressed genes (<i>p</i>-value < 0.05) when mapped to a reference transcriptome. Further, multiple differentially expressed genes were identified from a custom <i>de novo</i> transcriptome, which was assembled using the reads that did not align with the reference genome. Thirteen of the identified transcripts, possibly involved in immunity, were validated by qRT-PCR. Homologues of seven of these genes were also found to be significantly upregulated in <i>Aedes aegypti</i> midguts 2 dpi, indicating a conserved mechanism at play. These results will help us to characterise the molecular interaction between <i>Aedes albopictus</i> and CHIKV and can be utilised to reduce the impact of this viral infection. |
topic |
Chikungunya <i>Aedes albopictus</i> RNASeq Host–pathogen interactions |
url |
https://www.mdpi.com/1999-4915/11/6/513 |
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