Polyinosinic-polycytidylic Acid Mediates PD-1 Expression in T Cells at Early Stage of Activation and Inhibits Melanoma Growth
Objective To investigate the expression level of programmed cell death protein 1 (PD-1) in T cells at the early stage of activation (T cells remained in spleen, before migration to target organ). Methods C57BL/6 mice (6-8 weeks old) were immunized with Poly I:C peptide vaccine after randomly groupin...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
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Magazine House of Cancer Research on Prevention and Treatment
2019-03-01
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| Series: | Zhongliu Fangzhi Yanjiu |
| Subjects: | |
| Online Access: | http://html.rhhz.net/ZLFZYJ/html/8578.2019.18.1213.htm |
| Summary: | Objective To investigate the expression level of programmed cell death protein 1 (PD-1) in T cells at the early stage of activation (T cells remained in spleen, before migration to target organ). Methods C57BL/6 mice (6-8 weeks old) were immunized with Poly I:C peptide vaccine after randomly grouping. Mice spleen cells were harvested 7 days after immunization. The effects of Poly I:C on the activation of antigen specific or nonspecific T cells were evaluated. PD-1 expression on the antigen specific CD8+T cells was also detected. Furthermore, correlation between the anti-tumor effects of Poly I:C and CD8+T cells was assessed by CD8 cell knockout. Results Poly I:C significantly promoted the activation of antigen specific or nonspecific T cells in mice spleens. Although antigen specific T cells increased the expression of PD-1, they could still synthesize IFN-γ, a T cell functional marker. Meanwhile, Poly I:C significantly inhibited the growth of melanoma in vivo (P=0.0243), which was correlated with CD8+T cells. Conclusion Poly I:C could promote the activation of T cells; although, T cells at the early stage of activation (remained in spleen) enhanced the expression of PD-1, they are still functional. |
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| ISSN: | 1000-8578 1000-8578 |