Quick assessment of influenza a virus infectivity with a long-range reverse-transcription quantitative polymerase chain reaction assay

Quick assessment of influenza a virus infectivity with a long-range reverse-transcription quantitative polymerase chain reaction assay

Abstract Background The polymerase chain reaction (PCR) is commonly used to detect viral pathogens because of its high sensitivity and specificity. However, conventional PCR methods cannot determine virus infectivity. Virus infectivity is conventionally examined with methods such as the plaque assay...

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Bibliographic Details
Main Authors: Yuki Nakaya, Takashi Fukuda, Hiroki Ashiba, Masato Yasuura, Makoto Fujimaki
Format: Article
Language:English
Published: BMC 2020-08-01
Series:BMC Infectious Diseases
Subjects:
Influenza virus
Ultraviolet rays
Long-range RT-qPCR
Infectivity
Strand break
Water treatment
Online Access:http://link.springer.com/article/10.1186/s12879-020-05317-8

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http://link.springer.com/article/10.1186/s12879-020-05317-8

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