Photoperiodic Response of In Vitro Cannabis sativa Plants
The majority of commercial Cannabis sativa L. (cannabis) cultivators use a 12.0-hour uninterrupted dark period to induce flowering; however, scientific information to prove this is the optimal dark period for all genotypes is lacking. Knowing genotype-specific photoperiods may help to promote growth...
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American Society for Horticultural Science (ASHS)
2020-12-01
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doaj-4ccd694acb61483ab580fa3f432e59972021-02-01T19:01:50ZengAmerican Society for Horticultural Science (ASHS)HortScience2327-98342020-12-01561108113https://doi.org/10.21273/HORTSCI15452-20Photoperiodic Response of In Vitro Cannabis sativa PlantsMelissa MoherMax JonesYoubin ZhengThe majority of commercial Cannabis sativa L. (cannabis) cultivators use a 12.0-hour uninterrupted dark period to induce flowering; however, scientific information to prove this is the optimal dark period for all genotypes is lacking. Knowing genotype-specific photoperiods may help to promote growth by providing the optimal photoperiod for photosynthesis. To determine whether the floral initiation of cannabis explants respond to varied photoperiods in vitro, explants were grown under one of six photoperiod treatments: 12.0, 13.2, 13.8, 14.4, 15.0, and 16.0 hours per day for 4 weeks. The percentage of flowering explants was highest under 12.0- and 13.2-hour treatments. There were no treatment effects on the fresh weight, final height, and growth index. Based on the results, it is recommended that an uninterrupted dark period of at least 10.8 hours (i.e., 13.2-hour photoperiod) be used to induce flowering for the ‘802’ genotype. In vitro flowering could provide a unique and high-throughput approach to study floral/seed development and secondary metabolism in cannabis under highly controlled conditions. Further research should determine if this response is the same on the whole-plant level.https://journals.ashs.org/hortsci/view/journals/hortsci/56/1/article-p108.xmlcannabisdark periodlower initiationphotoperiodtissue culture |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Melissa Moher Max Jones Youbin Zheng |
spellingShingle |
Melissa Moher Max Jones Youbin Zheng Photoperiodic Response of In Vitro Cannabis sativa Plants HortScience cannabis dark period lower initiation photoperiod tissue culture |
author_facet |
Melissa Moher Max Jones Youbin Zheng |
author_sort |
Melissa Moher |
title |
Photoperiodic Response of In Vitro Cannabis sativa Plants |
title_short |
Photoperiodic Response of In Vitro Cannabis sativa Plants |
title_full |
Photoperiodic Response of In Vitro Cannabis sativa Plants |
title_fullStr |
Photoperiodic Response of In Vitro Cannabis sativa Plants |
title_full_unstemmed |
Photoperiodic Response of In Vitro Cannabis sativa Plants |
title_sort |
photoperiodic response of in vitro cannabis sativa plants |
publisher |
American Society for Horticultural Science (ASHS) |
series |
HortScience |
issn |
2327-9834 |
publishDate |
2020-12-01 |
description |
The majority of commercial Cannabis sativa L. (cannabis) cultivators use a 12.0-hour uninterrupted dark period to induce flowering; however, scientific information to prove this is the optimal dark period for all genotypes is lacking. Knowing genotype-specific photoperiods may help to promote growth by providing the optimal photoperiod for photosynthesis. To determine whether the floral initiation of cannabis explants respond to varied photoperiods in vitro, explants were grown under one of six photoperiod treatments: 12.0, 13.2, 13.8, 14.4, 15.0, and 16.0 hours per day for 4 weeks. The percentage of flowering explants was highest under 12.0- and 13.2-hour treatments. There were no treatment effects on the fresh weight, final height, and growth index. Based on the results, it is recommended that an uninterrupted dark period of at least 10.8 hours (i.e., 13.2-hour photoperiod) be used to induce flowering for the ‘802’ genotype. In vitro flowering could provide a unique and high-throughput approach to study floral/seed development and secondary metabolism in cannabis under highly controlled conditions. Further research should determine if this response is the same on the whole-plant level. |
topic |
cannabis dark period lower initiation photoperiod tissue culture |
url |
https://journals.ashs.org/hortsci/view/journals/hortsci/56/1/article-p108.xml |
work_keys_str_mv |
AT melissamoher photoperiodicresponseofinvitrocannabissativaplants AT maxjones photoperiodicresponseofinvitrocannabissativaplants AT youbinzheng photoperiodicresponseofinvitrocannabissativaplants |
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