Differences in Hypertrophic Scar Fibroblasts according to Scar Severity: Expression of Transforming Growth Factor beta1 at the mRNA and Protein Levels
Background Hypertrophic scars result from excessive collagen deposition and increased transforming growth factor beta-1 (TGF-beta1) levels. We hypothesized that the expression of TGF-beta1 mRNA and protein would increase with the clinical severity of hypertrophic scars. Methods Primary dermal fibrob...
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Korean Society for Aesthetic Plastic Surgery
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Online Access: | http://e-aaps.org/upload/pdf/aaps-21-116.pdf |
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doaj-4cd50ee8372845e7a96165adb96fcf902020-11-25T03:00:18ZengKorean Society for Aesthetic Plastic SurgeryArchives of Aesthetic Plastic Surgery2234-08312288-93372015-10-0121311612010.14730/aaps.2015.21.3.11689Differences in Hypertrophic Scar Fibroblasts according to Scar Severity: Expression of Transforming Growth Factor beta1 at the mRNA and Protein LevelsSe Young Kim0Seung Min NamEun Soo Park1Yong Bae Kim2 Department of Plastic and Reconstructive Surgery, College of Medicine, Soonchunhyang University, Bucheon, Korea Department of Plastic and Reconstructive Surgery, College of Medicine, Soonchunhyang University, Bucheon, Korea Department of Plastic and Reconstructive Surgery, College of Medicine, Soonchunhyang University, Bucheon, KoreaBackground Hypertrophic scars result from excessive collagen deposition and increased transforming growth factor beta-1 (TGF-beta1) levels. We hypothesized that the expression of TGF-beta1 mRNA and protein would increase with the clinical severity of hypertrophic scars. Methods Primary dermal fibroblasts were isolated from cultures of normal skin and hypertrophic scars. The hypertrophic scars were classified by grade based on the Vancouver Scar Scale. After 96 hours of serum starvation, TGF-beta1 levels in the supernatant were determined using solid-phase, enzyme-linked immunosorbent assay (ELISA). Quantitative reverse transcription-polymerase chain reaction was performed to quantify TGF-beta1 mRNA expression. Results TGF-beta1 protein levels of hypertrophic scars tended to increase with increasing severity of the scars, according to the Vancouver Scar Scale. The differences between the normal dermal tissue (NS), hypertrophic scar grade (HS) 1, and HS4 groups were statistically significant (P<0.01). The TGF-beta1 mRNA levels of hypertrophic scars also tended to increase according to scar severity. The differences between the NS, HS1, HS2, HS3, and HS4 groups were statistically significant (P<0.01). Conclusions The classification of hypertrophic scars according to the Vancouver Scar Scale usually matches the severity of the microenvironment of the hypertrophic scar.http://e-aaps.org/upload/pdf/aaps-21-116.pdfhypertrophic scartransforming growth factor-betavancouver scar scale |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Se Young Kim Seung Min Nam Eun Soo Park Yong Bae Kim |
spellingShingle |
Se Young Kim Seung Min Nam Eun Soo Park Yong Bae Kim Differences in Hypertrophic Scar Fibroblasts according to Scar Severity: Expression of Transforming Growth Factor beta1 at the mRNA and Protein Levels Archives of Aesthetic Plastic Surgery hypertrophic scar transforming growth factor-beta vancouver scar scale |
author_facet |
Se Young Kim Seung Min Nam Eun Soo Park Yong Bae Kim |
author_sort |
Se Young Kim |
title |
Differences in Hypertrophic Scar Fibroblasts according to Scar Severity: Expression of Transforming Growth Factor beta1 at the mRNA and Protein Levels |
title_short |
Differences in Hypertrophic Scar Fibroblasts according to Scar Severity: Expression of Transforming Growth Factor beta1 at the mRNA and Protein Levels |
title_full |
Differences in Hypertrophic Scar Fibroblasts according to Scar Severity: Expression of Transforming Growth Factor beta1 at the mRNA and Protein Levels |
title_fullStr |
Differences in Hypertrophic Scar Fibroblasts according to Scar Severity: Expression of Transforming Growth Factor beta1 at the mRNA and Protein Levels |
title_full_unstemmed |
Differences in Hypertrophic Scar Fibroblasts according to Scar Severity: Expression of Transforming Growth Factor beta1 at the mRNA and Protein Levels |
title_sort |
differences in hypertrophic scar fibroblasts according to scar severity: expression of transforming growth factor beta1 at the mrna and protein levels |
publisher |
Korean Society for Aesthetic Plastic Surgery |
series |
Archives of Aesthetic Plastic Surgery |
issn |
2234-0831 2288-9337 |
publishDate |
2015-10-01 |
description |
Background Hypertrophic scars result from excessive collagen deposition and increased transforming growth factor beta-1 (TGF-beta1) levels. We hypothesized that the expression of TGF-beta1 mRNA and protein would increase with the clinical severity of hypertrophic scars. Methods Primary dermal fibroblasts were isolated from cultures of normal skin and hypertrophic scars. The hypertrophic scars were classified by grade based on the Vancouver Scar Scale. After 96 hours of serum starvation, TGF-beta1 levels in the supernatant were determined using solid-phase, enzyme-linked immunosorbent assay (ELISA). Quantitative reverse transcription-polymerase chain reaction was performed to quantify TGF-beta1 mRNA expression. Results TGF-beta1 protein levels of hypertrophic scars tended to increase with increasing severity of the scars, according to the Vancouver Scar Scale. The differences between the normal dermal tissue (NS), hypertrophic scar grade (HS) 1, and HS4 groups were statistically significant (P<0.01). The TGF-beta1 mRNA levels of hypertrophic scars also tended to increase according to scar severity. The differences between the NS, HS1, HS2, HS3, and HS4 groups were statistically significant (P<0.01). Conclusions The classification of hypertrophic scars according to the Vancouver Scar Scale usually matches the severity of the microenvironment of the hypertrophic scar. |
topic |
hypertrophic scar transforming growth factor-beta vancouver scar scale |
url |
http://e-aaps.org/upload/pdf/aaps-21-116.pdf |
work_keys_str_mv |
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