Allele and genotype frequencies of milkprotein kappa-casein (CSN3) in artifical insemination bulls of Czech Fleckvieh and Holstein breed

• Main Author: Jiří Bezdíček
• Format: Article
• Language: English
• Published: Mendel University Press 2007-01-01
• Series: Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
• Subjects: kappa-casein; allele frequencies; genotype frequencies; Czech Fleckvieh bulls; Holstein bulls
• Online Access: https://acta.mendelu.cz/55/5/0017/

This thesis was meant to evaluate allele and genotype frequencies of kappa-casein (CSN3) in artificial insemination bulls in the Czech Republic of Czech Fleckvieh breed (n = 133) and Holstein breed (n = 58). Studied bulls were born between 1990 and 2005. Calculation was made by PowerMarker (Liu K., Muse S.V. 2005). Genotype frequencies established in Czech Fleckvieh bulls were AA = 0.391 AB = 0.4887 AE = 0.0075 BB = 0.0902 BE = 0.0226 and EE = 0 and allele frequencies were A = 0.6391 B = 0.3459 E = 0.015. Genotype frequencies established in Holstein bulls were AA = 0.5517 AB = 0.2759 AE = 0.1207 BB = 0 BE = 0.0345 and EE = 0.0172 and allele frequencies A = 0.75 B = 0.1552 and E = 0.0948. In Holstein breed we found higher frequency of E allele and higher frequency of genotypes with this allele. Genetic diversity, heterozygosis and PIC values in Czech Fleckvieh Bulls were following 0.4717; 0.5188 and 0.3737 and 0.4044; 0.4310 and 0.3668 in Holstein Bulls. These results show the higher genetic variability in bulls of Czech Fleckvieh breed. Results of χ2 test (Czech Fleckvieh 4.58; Holstein 2.55) show genetical disequilibrium in the Bulls both examined breeds used in artificial insemination in the Czech Republic.