The Lifestyle-Dependent Microbial Interactions Vary Between Upstream and Downstream of the Three Gorges Dam
Dams represent the most significant anthropogenic disturbance to global rivers. Previous studies have shown that free-living and particle-attached microbes exhibited differentially in river and reservoir ecosystems. However, little is known about the dam’s effect on their co-occurrence patterns. Her...
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doaj-4d26c9fc849446c4833aad73e1aaf1f32021-05-24T05:28:57ZengFrontiers Media S.A.Frontiers in Ecology and Evolution2296-701X2021-05-01910.3389/fevo.2021.624476624476The Lifestyle-Dependent Microbial Interactions Vary Between Upstream and Downstream of the Three Gorges DamShang Wang0Weiguo Hou1Hongchen Jiang2Hailiang Dong3Liuqin Huang4Shu Chen5Bin Wang6Yongcan Chen7Binliang Lin8Ye Deng9CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Beijing, ChinaSchool of Ocean Sciences, China University of Geosciences, Beijing, ChinaState Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Beijing, ChinaState Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, ChinaSchool of Environment and Resource, Southwest University of Science and Technology, Sichuan, ChinaSchool of Environment and Resource, Southwest University of Science and Technology, Sichuan, ChinaSchool of Environment and Resource, Southwest University of Science and Technology, Sichuan, ChinaDepartment of Hydraulic Engineering, Tsinghua University, Beijing, ChinaCAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, ChinaDams represent the most significant anthropogenic disturbance to global rivers. Previous studies have shown that free-living and particle-attached microbes exhibited differentially in river and reservoir ecosystems. However, little is known about the dam’s effect on their co-occurrence patterns. Here, a random matrix theory (RMT)-based network approach was used to construct microbial ecological networks for free-living and particle-attached communities in the immediate vicinity of the Three Gorges Dam (TGD), based on a high-throughput sequencing of 16S rRNA gene. Microbial distribution pattern showed that differences caused by lifestyle (free-living vs. particle-attached) were greater than those caused by geographic position (upstream vs. downstream of the TGD). Network analysis revealed higher connectivity and a lower number of modules in the overall downstream networks. Furthermore, considering the lifestyle, the network structures and properties for free-living and particle-attached microbes were different between upstream and downstream of the dam. Specifically, free-living communities located upstream of the dam exhibited a more complex co-occurrence pattern than the particle-attached communities, whereas the opposite was true for those located downstream of the dam. This variation indicated a strong impact of the dam on microbial interactions for microbes with similar lifestyle in the vicinity of the dam. We identified 112 persistent operational taxonomic unit (OTU)-level species that stably coexisted regardless of lifestyle and geographic positions. These persistent species occupied 21.33–25.57% of the total nodes in each network, and together with their first neighbors, they contributed more than 50% of the nodes and edges belonging to each network. Furthermore, we found that taxonomic affiliations for central nodes (with high degree) varied in these persistent species sub-networks. Collectively, our findings expand the current understanding of the dam’s effect on species interaction variation patterns for free-living and particle-attached communities in the vicinity of the dam, which are more complex than traditional alpha and beta microbial diversity.https://www.frontiersin.org/articles/10.3389/fevo.2021.624476/fullmicrobial interactionthe Three Gorges Damfree-livingparticle-attachedpersistent speciesRMT-based network |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shang Wang Weiguo Hou Hongchen Jiang Hailiang Dong Liuqin Huang Shu Chen Bin Wang Yongcan Chen Binliang Lin Ye Deng |
spellingShingle |
Shang Wang Weiguo Hou Hongchen Jiang Hailiang Dong Liuqin Huang Shu Chen Bin Wang Yongcan Chen Binliang Lin Ye Deng The Lifestyle-Dependent Microbial Interactions Vary Between Upstream and Downstream of the Three Gorges Dam Frontiers in Ecology and Evolution microbial interaction the Three Gorges Dam free-living particle-attached persistent species RMT-based network |
author_facet |
Shang Wang Weiguo Hou Hongchen Jiang Hailiang Dong Liuqin Huang Shu Chen Bin Wang Yongcan Chen Binliang Lin Ye Deng |
author_sort |
Shang Wang |
title |
The Lifestyle-Dependent Microbial Interactions Vary Between Upstream and Downstream of the Three Gorges Dam |
title_short |
The Lifestyle-Dependent Microbial Interactions Vary Between Upstream and Downstream of the Three Gorges Dam |
title_full |
The Lifestyle-Dependent Microbial Interactions Vary Between Upstream and Downstream of the Three Gorges Dam |
title_fullStr |
The Lifestyle-Dependent Microbial Interactions Vary Between Upstream and Downstream of the Three Gorges Dam |
title_full_unstemmed |
The Lifestyle-Dependent Microbial Interactions Vary Between Upstream and Downstream of the Three Gorges Dam |
title_sort |
lifestyle-dependent microbial interactions vary between upstream and downstream of the three gorges dam |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Ecology and Evolution |
issn |
2296-701X |
publishDate |
2021-05-01 |
description |
Dams represent the most significant anthropogenic disturbance to global rivers. Previous studies have shown that free-living and particle-attached microbes exhibited differentially in river and reservoir ecosystems. However, little is known about the dam’s effect on their co-occurrence patterns. Here, a random matrix theory (RMT)-based network approach was used to construct microbial ecological networks for free-living and particle-attached communities in the immediate vicinity of the Three Gorges Dam (TGD), based on a high-throughput sequencing of 16S rRNA gene. Microbial distribution pattern showed that differences caused by lifestyle (free-living vs. particle-attached) were greater than those caused by geographic position (upstream vs. downstream of the TGD). Network analysis revealed higher connectivity and a lower number of modules in the overall downstream networks. Furthermore, considering the lifestyle, the network structures and properties for free-living and particle-attached microbes were different between upstream and downstream of the dam. Specifically, free-living communities located upstream of the dam exhibited a more complex co-occurrence pattern than the particle-attached communities, whereas the opposite was true for those located downstream of the dam. This variation indicated a strong impact of the dam on microbial interactions for microbes with similar lifestyle in the vicinity of the dam. We identified 112 persistent operational taxonomic unit (OTU)-level species that stably coexisted regardless of lifestyle and geographic positions. These persistent species occupied 21.33–25.57% of the total nodes in each network, and together with their first neighbors, they contributed more than 50% of the nodes and edges belonging to each network. Furthermore, we found that taxonomic affiliations for central nodes (with high degree) varied in these persistent species sub-networks. Collectively, our findings expand the current understanding of the dam’s effect on species interaction variation patterns for free-living and particle-attached communities in the vicinity of the dam, which are more complex than traditional alpha and beta microbial diversity. |
topic |
microbial interaction the Three Gorges Dam free-living particle-attached persistent species RMT-based network |
url |
https://www.frontiersin.org/articles/10.3389/fevo.2021.624476/full |
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