Evaluation of intestinal microbiota, short-chain fatty acids, and immunoglobulin a in diversion colitis
It is reported that an increase in aerobic bacteria, a lack of short-chain fatty acids (SCFAs), and immune disorders in the diverted colon are major causes of diversion colitis. However, the precise pathogenesis of this condition remains unclear. The aim of the present study was to examine the micro...
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Format: | Article |
Language: | English |
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Elsevier
2021-03-01
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Series: | Biochemistry and Biophysics Reports |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580820302028 |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kentaro Tominaga Atsunori Tsuchiya Takeshi Mizusawa Asami Matsumoto Ayaka Minemura Kentaro Oka Motomichi Takahashi Tomoaki Yosida Yuzo Kawata Kazuya Takahashi Hiroki Sato Satoshi Ikarashi Kazunao Hayashi Ken-ichi Mizuno Yosuke Tajima Masato Nakano Yoshifumi Shimada Hitoshi Kameyama Junji Yokoyama Toshifumi Wakai Shuji Terai |
spellingShingle |
Kentaro Tominaga Atsunori Tsuchiya Takeshi Mizusawa Asami Matsumoto Ayaka Minemura Kentaro Oka Motomichi Takahashi Tomoaki Yosida Yuzo Kawata Kazuya Takahashi Hiroki Sato Satoshi Ikarashi Kazunao Hayashi Ken-ichi Mizuno Yosuke Tajima Masato Nakano Yoshifumi Shimada Hitoshi Kameyama Junji Yokoyama Toshifumi Wakai Shuji Terai Evaluation of intestinal microbiota, short-chain fatty acids, and immunoglobulin a in diversion colitis Biochemistry and Biophysics Reports Diversion colitis Microbiota Short-chain fatty acids Immunoglobulin A |
author_facet |
Kentaro Tominaga Atsunori Tsuchiya Takeshi Mizusawa Asami Matsumoto Ayaka Minemura Kentaro Oka Motomichi Takahashi Tomoaki Yosida Yuzo Kawata Kazuya Takahashi Hiroki Sato Satoshi Ikarashi Kazunao Hayashi Ken-ichi Mizuno Yosuke Tajima Masato Nakano Yoshifumi Shimada Hitoshi Kameyama Junji Yokoyama Toshifumi Wakai Shuji Terai |
author_sort |
Kentaro Tominaga |
title |
Evaluation of intestinal microbiota, short-chain fatty acids, and immunoglobulin a in diversion colitis |
title_short |
Evaluation of intestinal microbiota, short-chain fatty acids, and immunoglobulin a in diversion colitis |
title_full |
Evaluation of intestinal microbiota, short-chain fatty acids, and immunoglobulin a in diversion colitis |
title_fullStr |
Evaluation of intestinal microbiota, short-chain fatty acids, and immunoglobulin a in diversion colitis |
title_full_unstemmed |
Evaluation of intestinal microbiota, short-chain fatty acids, and immunoglobulin a in diversion colitis |
title_sort |
evaluation of intestinal microbiota, short-chain fatty acids, and immunoglobulin a in diversion colitis |
publisher |
Elsevier |
series |
Biochemistry and Biophysics Reports |
issn |
2405-5808 |
publishDate |
2021-03-01 |
description |
It is reported that an increase in aerobic bacteria, a lack of short-chain fatty acids (SCFAs), and immune disorders in the diverted colon are major causes of diversion colitis. However, the precise pathogenesis of this condition remains unclear. The aim of the present study was to examine the microbiota, intestinal SCFAs, and immunoglobulin A (IgA) in the diverted colon. Eight patients underwent operative procedures for colostomies. We assessed the diverted colon using endoscopy and obtained intestinal samples from the diverted colon and oral colon in these patients. We analyzed the microbiota and SCFAs of the intestinal samples. The bacterial communities were investigated using a 16S rRNA gene sequencing method. The microbiota demonstrated a change in the proportion of some species, especially Lactobacillus, which significantly decreased in the diverted colon at the genus level. We also showed that intestinal SCFA values were significantly decreased in the diverted colon. Furthermore, intestinal IgA levels were significantly increased in the diverted colon. This study was the first to show that intestinal SCFAs were significantly decreased and intestinal IgA was significantly increased in the diverted colon. Our data suggest that SCFAs affect the microbiota and may play an immunological role in diversion colitis. |
topic |
Diversion colitis Microbiota Short-chain fatty acids Immunoglobulin A |
url |
http://www.sciencedirect.com/science/article/pii/S2405580820302028 |
work_keys_str_mv |
AT kentarotominaga evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT atsunoritsuchiya evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT takeshimizusawa evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT asamimatsumoto evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT ayakaminemura evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT kentarooka evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT motomichitakahashi evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT tomoakiyosida evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT yuzokawata evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT kazuyatakahashi evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT hirokisato evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT satoshiikarashi evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT kazunaohayashi evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT kenichimizuno evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT yosuketajima evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT masatonakano evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT yoshifumishimada evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT hitoshikameyama evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT junjiyokoyama evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT toshifumiwakai evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis AT shujiterai evaluationofintestinalmicrobiotashortchainfattyacidsandimmunoglobulinaindiversioncolitis |
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1724274594932588544 |
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doaj-4d395b1d532e4444b5ab20836e1c16732021-02-11T04:22:19ZengElsevierBiochemistry and Biophysics Reports2405-58082021-03-0125100892Evaluation of intestinal microbiota, short-chain fatty acids, and immunoglobulin a in diversion colitisKentaro Tominaga0Atsunori Tsuchiya1Takeshi Mizusawa2Asami Matsumoto3Ayaka Minemura4Kentaro Oka5Motomichi Takahashi6Tomoaki Yosida7Yuzo Kawata8Kazuya Takahashi9Hiroki Sato10Satoshi Ikarashi11Kazunao Hayashi12Ken-ichi Mizuno13Yosuke Tajima14Masato Nakano15Yoshifumi Shimada16Hitoshi Kameyama17Junji Yokoyama18Toshifumi Wakai19Shuji Terai20Division of Gastroenterology and Hepatology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, JapanDivision of Gastroenterology and Hepatology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan; Corresponding author. Division of Gastroenterology and Hepatology Graduate School of Medical and Dental Sciences Niigata University, 1–757 Asahimachi–dori Chuo–ku, Niigata, 9518510, Japan.Division of Gastroenterology and Hepatology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, JapanResearch Department, R&D Division, Miyarisan Pharmaceutical Co., Ltd., Saitama, JapanResearch Department, R&D Division, Miyarisan Pharmaceutical Co., Ltd., Saitama, JapanResearch Department, R&D Division, Miyarisan Pharmaceutical Co., Ltd., Saitama, JapanResearch Department, R&D Division, Miyarisan Pharmaceutical Co., Ltd., Saitama, JapanDivision of Gastroenterology and Hepatology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, JapanDivision of Gastroenterology and Hepatology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, JapanDivision of Gastroenterology and Hepatology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, JapanDivision of Gastroenterology and Hepatology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, JapanDivision of Gastroenterology and Hepatology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, JapanDivision of Gastroenterology and Hepatology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, JapanDivision of Gastroenterology and Hepatology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, JapanDivision of Digestive and General Surgery, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, JapanDivision of Digestive and General Surgery, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, JapanDivision of Digestive and General Surgery, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, JapanDivision of Digestive and General Surgery, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, JapanDivision of Gastroenterology and Hepatology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, JapanDivision of Digestive and General Surgery, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, JapanDivision of Gastroenterology and Hepatology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan; Corresponding author. Division of Gastroenterology and Hepatology Graduate School of Medical and Dental Sciences Niigata University, 1–757 Asahimachi–dori Chuo–ku, Niigata, 9518510, Japan.It is reported that an increase in aerobic bacteria, a lack of short-chain fatty acids (SCFAs), and immune disorders in the diverted colon are major causes of diversion colitis. However, the precise pathogenesis of this condition remains unclear. The aim of the present study was to examine the microbiota, intestinal SCFAs, and immunoglobulin A (IgA) in the diverted colon. Eight patients underwent operative procedures for colostomies. We assessed the diverted colon using endoscopy and obtained intestinal samples from the diverted colon and oral colon in these patients. We analyzed the microbiota and SCFAs of the intestinal samples. The bacterial communities were investigated using a 16S rRNA gene sequencing method. The microbiota demonstrated a change in the proportion of some species, especially Lactobacillus, which significantly decreased in the diverted colon at the genus level. We also showed that intestinal SCFA values were significantly decreased in the diverted colon. Furthermore, intestinal IgA levels were significantly increased in the diverted colon. This study was the first to show that intestinal SCFAs were significantly decreased and intestinal IgA was significantly increased in the diverted colon. Our data suggest that SCFAs affect the microbiota and may play an immunological role in diversion colitis.http://www.sciencedirect.com/science/article/pii/S2405580820302028Diversion colitisMicrobiotaShort-chain fatty acidsImmunoglobulin A |