Genome-Wide Analysis of Respiratory Burst Oxidase Homologs in Grape (Vitis vinifera L.)
Plant respiratory burst oxidase homolog (rboh) genes appear to play crucial roles in plant development, defense reactions and hormone signaling. In this study, a total of seven rboh genes from grape were identified and characterized. Genomic structure and predicted protein sequence analysis indicate...
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doaj-4d423a2a0b7345318ac9004ee9b1d5072020-11-25T02:46:26ZengMDPI AGInternational Journal of Molecular Sciences1422-00672013-12-011412241692418610.3390/ijms141224169ijms141224169Genome-Wide Analysis of Respiratory Burst Oxidase Homologs in Grape (Vitis vinifera L.)Chenxia Cheng0Xiaozhao Xu1Min Gao2Jun Li3Chunlei Guo4Junyang Song5Xiping Wang6State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, ChinaPlant respiratory burst oxidase homolog (rboh) genes appear to play crucial roles in plant development, defense reactions and hormone signaling. In this study, a total of seven rboh genes from grape were identified and characterized. Genomic structure and predicted protein sequence analysis indicated that the sequences of plant rboh genes are highly conserved. Synteny analysis demonstrated that several Vvrboh genes were found in corresponding syntenic blocks of Arabidopsis, suggesting that these genes arose before the divergence of the respective lineages. The expression pattern of Vvrboh genes in different tissues was assessed by qRT-PCR and two were constitutively expressed in all tissues tested. The expression profiles were similarly analyzed following exposure to various stresses and hormone treatments. It was shown that the expression levels of VvrbohA, VvrbohB and VvrbohC1 were significantly increased by salt and drought treatments. VvrbohB, VvrbohC2, and VvrbohD exhibited a dramatic up-regulation after powdery mildew (Uncinula necator (Schw.) Burr.) inoculation, while VvrbohH was down-regulated. Finally, salicylic acid treatment strongly stimulated the expression of VvrbohD and VvrbohH, while abscisic acid treatment induced the expression of VvrbohB and VvrbohH. These results demonstrate that the expression patterns of grape rboh genes exhibit diverse and complex stress-response expression signatures.http://www.mdpi.com/1422-0067/14/12/24169reactive oxygen speciessynteny analysisphylogenetic analysisgene expression |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chenxia Cheng Xiaozhao Xu Min Gao Jun Li Chunlei Guo Junyang Song Xiping Wang |
spellingShingle |
Chenxia Cheng Xiaozhao Xu Min Gao Jun Li Chunlei Guo Junyang Song Xiping Wang Genome-Wide Analysis of Respiratory Burst Oxidase Homologs in Grape (Vitis vinifera L.) International Journal of Molecular Sciences reactive oxygen species synteny analysis phylogenetic analysis gene expression |
author_facet |
Chenxia Cheng Xiaozhao Xu Min Gao Jun Li Chunlei Guo Junyang Song Xiping Wang |
author_sort |
Chenxia Cheng |
title |
Genome-Wide Analysis of Respiratory Burst Oxidase Homologs in Grape (Vitis vinifera L.) |
title_short |
Genome-Wide Analysis of Respiratory Burst Oxidase Homologs in Grape (Vitis vinifera L.) |
title_full |
Genome-Wide Analysis of Respiratory Burst Oxidase Homologs in Grape (Vitis vinifera L.) |
title_fullStr |
Genome-Wide Analysis of Respiratory Burst Oxidase Homologs in Grape (Vitis vinifera L.) |
title_full_unstemmed |
Genome-Wide Analysis of Respiratory Burst Oxidase Homologs in Grape (Vitis vinifera L.) |
title_sort |
genome-wide analysis of respiratory burst oxidase homologs in grape (vitis vinifera l.) |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2013-12-01 |
description |
Plant respiratory burst oxidase homolog (rboh) genes appear to play crucial roles in plant development, defense reactions and hormone signaling. In this study, a total of seven rboh genes from grape were identified and characterized. Genomic structure and predicted protein sequence analysis indicated that the sequences of plant rboh genes are highly conserved. Synteny analysis demonstrated that several Vvrboh genes were found in corresponding syntenic blocks of Arabidopsis, suggesting that these genes arose before the divergence of the respective lineages. The expression pattern of Vvrboh genes in different tissues was assessed by qRT-PCR and two were constitutively expressed in all tissues tested. The expression profiles were similarly analyzed following exposure to various stresses and hormone treatments. It was shown that the expression levels of VvrbohA, VvrbohB and VvrbohC1 were significantly increased by salt and drought treatments. VvrbohB, VvrbohC2, and VvrbohD exhibited a dramatic up-regulation after powdery mildew (Uncinula necator (Schw.) Burr.) inoculation, while VvrbohH was down-regulated. Finally, salicylic acid treatment strongly stimulated the expression of VvrbohD and VvrbohH, while abscisic acid treatment induced the expression of VvrbohB and VvrbohH. These results demonstrate that the expression patterns of grape rboh genes exhibit diverse and complex stress-response expression signatures. |
topic |
reactive oxygen species synteny analysis phylogenetic analysis gene expression |
url |
http://www.mdpi.com/1422-0067/14/12/24169 |
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