Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer Embryos
Cloned pigs generated by somatic cell nuclear transfer (SCNT) show a greater ratio of early abortion during mid-gestation than normal controls. X-linked genes have been demonstrated to be important for the development of cloned embryos. To determine the relationship between the expression of X-linke...
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doaj-4d5ad1da35524d13b8adebbc42428b1c2020-11-25T01:01:30ZengMDPI AGInternational Journal of Molecular Sciences1422-00672014-11-011512216312164310.3390/ijms151221631ijms151221631Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer EmbryosLin Yuan0Anfeng Wang1Chaogang Yao2Yongye Huang3Feifei Duan4Qinyan Lv5Dongxu Wang6Hongsheng Ouyang7Zhanjun Li8Liangxue Lai9Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaCloned pigs generated by somatic cell nuclear transfer (SCNT) show a greater ratio of early abortion during mid-gestation than normal controls. X-linked genes have been demonstrated to be important for the development of cloned embryos. To determine the relationship between the expression of X-linked genes and abortion of cloned porcine fetuses, the expression of X-linked genes were investigated by quantitative real-time polymerase chain reaction (q-PCR) and the methylation status of Xist DMR was performed by bisulfate-specific PCR (BSP). q-PCR analysis indicated that there was aberrant expression of X-linked genes, especially the upregulated expression of Xist in both female and male aborted fetuses compared to control fetuses. Results of BSP suggested that hypomethylation of Xist occurred in aborted fetuses, whether male or female. These results suggest that the abnormal expression of Xist may be associated with the abortion of fetuses derived from somatic cell nuclear transfer embryos.http://www.mdpi.com/1422-0067/15/12/21631porcinesomatic cell nuclear transferDNA methylationXist |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lin Yuan Anfeng Wang Chaogang Yao Yongye Huang Feifei Duan Qinyan Lv Dongxu Wang Hongsheng Ouyang Zhanjun Li Liangxue Lai |
spellingShingle |
Lin Yuan Anfeng Wang Chaogang Yao Yongye Huang Feifei Duan Qinyan Lv Dongxu Wang Hongsheng Ouyang Zhanjun Li Liangxue Lai Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer Embryos International Journal of Molecular Sciences porcine somatic cell nuclear transfer DNA methylation Xist |
author_facet |
Lin Yuan Anfeng Wang Chaogang Yao Yongye Huang Feifei Duan Qinyan Lv Dongxu Wang Hongsheng Ouyang Zhanjun Li Liangxue Lai |
author_sort |
Lin Yuan |
title |
Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer Embryos |
title_short |
Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer Embryos |
title_full |
Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer Embryos |
title_fullStr |
Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer Embryos |
title_full_unstemmed |
Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer Embryos |
title_sort |
aberrant expression of xist in aborted porcine fetuses derived from somatic cell nuclear transfer embryos |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2014-11-01 |
description |
Cloned pigs generated by somatic cell nuclear transfer (SCNT) show a greater ratio of early abortion during mid-gestation than normal controls. X-linked genes have been demonstrated to be important for the development of cloned embryos. To determine the relationship between the expression of X-linked genes and abortion of cloned porcine fetuses, the expression of X-linked genes were investigated by quantitative real-time polymerase chain reaction (q-PCR) and the methylation status of Xist DMR was performed by bisulfate-specific PCR (BSP). q-PCR analysis indicated that there was aberrant expression of X-linked genes, especially the upregulated expression of Xist in both female and male aborted fetuses compared to control fetuses. Results of BSP suggested that hypomethylation of Xist occurred in aborted fetuses, whether male or female. These results suggest that the abnormal expression of Xist may be associated with the abortion of fetuses derived from somatic cell nuclear transfer embryos. |
topic |
porcine somatic cell nuclear transfer DNA methylation Xist |
url |
http://www.mdpi.com/1422-0067/15/12/21631 |
work_keys_str_mv |
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