Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer Embryos

Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer Embryos

Cloned pigs generated by somatic cell nuclear transfer (SCNT) show a greater ratio of early abortion during mid-gestation than normal controls. X-linked genes have been demonstrated to be important for the development of cloned embryos. To determine the relationship between the expression of X-linke...

Full description

Bibliographic Details
Main Authors: Lin Yuan, Anfeng Wang, Chaogang Yao, Yongye Huang, Feifei Duan, Qinyan Lv, Dongxu Wang, Hongsheng Ouyang, Zhanjun Li, Liangxue Lai
Format: Article
Language:English
Published: MDPI AG 2014-11-01
Series:International Journal of Molecular Sciences
Subjects:
porcine
somatic cell nuclear transfer
DNA methylation
Xist
Online Access:http://www.mdpi.com/1422-0067/15/12/21631
id doaj-4d5ad1da35524d13b8adebbc42428b1c
record_format Article
spelling doaj-4d5ad1da35524d13b8adebbc42428b1c2020-11-25T01:01:30ZengMDPI AGInternational Journal of Molecular Sciences1422-00672014-11-011512216312164310.3390/ijms151221631ijms151221631Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer EmbryosLin Yuan0Anfeng Wang1Chaogang Yao2Yongye Huang3Feifei Duan4Qinyan Lv5Dongxu Wang6Hongsheng Ouyang7Zhanjun Li8Liangxue Lai9Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, ChinaCloned pigs generated by somatic cell nuclear transfer (SCNT) show a greater ratio of early abortion during mid-gestation than normal controls. X-linked genes have been demonstrated to be important for the development of cloned embryos. To determine the relationship between the expression of X-linked genes and abortion of cloned porcine fetuses, the expression of X-linked genes were investigated by quantitative real-time polymerase chain reaction (q-PCR) and the methylation status of Xist DMR was performed by bisulfate-specific PCR (BSP). q-PCR analysis indicated that there was aberrant expression of X-linked genes, especially the upregulated expression of Xist in both female and male aborted fetuses compared to control fetuses. Results of BSP suggested that hypomethylation of Xist occurred in aborted fetuses, whether male or female. These results suggest that the abnormal expression of Xist may be associated with the abortion of fetuses derived from somatic cell nuclear transfer embryos.http://www.mdpi.com/1422-0067/15/12/21631porcinesomatic cell nuclear transferDNA methylationXist
collection DOAJ
language English
format Article
sources DOAJ
author Lin Yuan
Anfeng Wang
Chaogang Yao
Yongye Huang
Feifei Duan
Qinyan Lv
Dongxu Wang
Hongsheng Ouyang
Zhanjun Li
Liangxue Lai
spellingShingle Lin Yuan
Anfeng Wang
Chaogang Yao
Yongye Huang
Feifei Duan
Qinyan Lv
Dongxu Wang
Hongsheng Ouyang
Zhanjun Li
Liangxue Lai
Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer Embryos
International Journal of Molecular Sciences
porcine
somatic cell nuclear transfer
DNA methylation
Xist
author_facet Lin Yuan
Anfeng Wang
Chaogang Yao
Yongye Huang
Feifei Duan
Qinyan Lv
Dongxu Wang
Hongsheng Ouyang
Zhanjun Li
Liangxue Lai
author_sort Lin Yuan
title Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer Embryos
title_short Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer Embryos
title_full Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer Embryos
title_fullStr Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer Embryos
title_full_unstemmed Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer Embryos
title_sort aberrant expression of xist in aborted porcine fetuses derived from somatic cell nuclear transfer embryos
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2014-11-01
description Cloned pigs generated by somatic cell nuclear transfer (SCNT) show a greater ratio of early abortion during mid-gestation than normal controls. X-linked genes have been demonstrated to be important for the development of cloned embryos. To determine the relationship between the expression of X-linked genes and abortion of cloned porcine fetuses, the expression of X-linked genes were investigated by quantitative real-time polymerase chain reaction (q-PCR) and the methylation status of Xist DMR was performed by bisulfate-specific PCR (BSP). q-PCR analysis indicated that there was aberrant expression of X-linked genes, especially the upregulated expression of Xist in both female and male aborted fetuses compared to control fetuses. Results of BSP suggested that hypomethylation of Xist occurred in aborted fetuses, whether male or female. These results suggest that the abnormal expression of Xist may be associated with the abortion of fetuses derived from somatic cell nuclear transfer embryos.
topic porcine
somatic cell nuclear transfer
DNA methylation
Xist
url http://www.mdpi.com/1422-0067/15/12/21631
work_keys_str_mv AT linyuan aberrantexpressionofxistinabortedporcinefetusesderivedfromsomaticcellnucleartransferembryos
AT anfengwang aberrantexpressionofxistinabortedporcinefetusesderivedfromsomaticcellnucleartransferembryos
AT chaogangyao aberrantexpressionofxistinabortedporcinefetusesderivedfromsomaticcellnucleartransferembryos
AT yongyehuang aberrantexpressionofxistinabortedporcinefetusesderivedfromsomaticcellnucleartransferembryos
AT feifeiduan aberrantexpressionofxistinabortedporcinefetusesderivedfromsomaticcellnucleartransferembryos
AT qinyanlv aberrantexpressionofxistinabortedporcinefetusesderivedfromsomaticcellnucleartransferembryos
AT dongxuwang aberrantexpressionofxistinabortedporcinefetusesderivedfromsomaticcellnucleartransferembryos
AT hongshengouyang aberrantexpressionofxistinabortedporcinefetusesderivedfromsomaticcellnucleartransferembryos
AT zhanjunli aberrantexpressionofxistinabortedporcinefetusesderivedfromsomaticcellnucleartransferembryos
AT liangxuelai aberrantexpressionofxistinabortedporcinefetusesderivedfromsomaticcellnucleartransferembryos
_version_ 1725208973421838336

Similar Items