In Vitro Evaluation of Dentin Tubule Occlusion for Novel Calcium Lactate Phosphate (CLP) Paste

• Main Authors: Jen-Chang Yang, Hsin-Tai Hu, Sheng-Yang Lee, Sung-Chih Hsieh, Pei-Chi Huang, Chen-Feng Ma, Dian-Yu Ji, Liang-Yu Chang, Nai-Chia Teng
• Format: Article
• Language: English
• Published: MDPI AG 2017-02-01
• Series: Materials
• Subjects: dentin hypersensitivity; calcium lactate phosphate (CLP); desensitizing agent
• Online Access: http://www.mdpi.com/1996-1944/10/3/228

Introduction: The objective of this in vitro study is to evaluate the effective and long-term occlusion of dentinal tubules using a novel calcium lactate phosphate (CLP) based desensitizing agent. Methods: Dentin disks (n = 9) were pre-etched using 1 M lactic acid for 30 s and individually treated with Colgate® Pro-Relief™ paste, CLP paste, and double distilled water (ddH2O) by a rubber-cupped handpiece. Dentin disks were analyzed under optical micrographs for pre-treatment, directly after treatment, and 14 days post-treatment. One-way ANOVA and post-hoc Tukey’s test were used to determine whether there were any statistically significant differences in dentinal tubule diameter. Results: A significant decrease occurred in the mean tubule diameter for dentin disks treated with CLP paste. A decrease was observed from 3.52 ± 0.83 µm to 2.62 ± 0.42 µm right after treatment, further decreasing to 1.71 ± 0.45 µm after immersion in artificial saliva for 14 days (p < 0.05). Conclusions: The results suggest that the CLP based desensitizing paste has remineralization properties and provides instant and lasting effectiveness in dentinal tubule occlusion.