Analysis of Complete Genomes of Propionibacterium acnes Reveals a Novel Plasmid and Increased Pseudogenes in an Acne Associated Strain
The human skin harbors a diverse community of bacteria, including the Gram-positive, anaerobic bacterium Propionibacterium acnes. P. acnes has historically been linked to the pathogenesis of acne vulgaris, a common skin disease affecting over 80% of all adolescents in the US. To gain insight into po...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2013-01-01
|
Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2013/918320 |
id |
doaj-4d640c35353a401f832071f5685945ca |
---|---|
record_format |
Article |
spelling |
doaj-4d640c35353a401f832071f5685945ca2020-11-24T22:30:40ZengHindawi LimitedBioMed Research International2314-61332314-61412013-01-01201310.1155/2013/918320918320Analysis of Complete Genomes of Propionibacterium acnes Reveals a Novel Plasmid and Increased Pseudogenes in an Acne Associated StrainGabriela Kasimatis0Sorel Fitz-Gibbon1Shuta Tomida2Marthew Wong3Huiying Li4Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California, Los Angeles, CA 90095, USADepartment of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California, Los Angeles, CA 90095, USADepartment of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California, Los Angeles, CA 90095, USADepartment of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California, Los Angeles, CA 90095, USADepartment of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California, Los Angeles, CA 90095, USAThe human skin harbors a diverse community of bacteria, including the Gram-positive, anaerobic bacterium Propionibacterium acnes. P. acnes has historically been linked to the pathogenesis of acne vulgaris, a common skin disease affecting over 80% of all adolescents in the US. To gain insight into potential P. acnes pathogenic mechanisms, we previously sequenced the complete genome of a P. acnes strain HL096PA1 that is highly associated with acne. In this study, we compared its genome to the first published complete genome KPA171202. HL096PA1 harbors a linear plasmid, pIMPLE-HL096PA1. This is the first described P. acnes plasmid. We also observed a five-fold increase of pseudogenes in HL096PA1, several of which encode proteins in carbohydrate transport and metabolism. In addition, our analysis revealed a few island-like genomic regions that are unique to HL096PA1 and a large genomic inversion spanning the ribosomal operons. Together, these findings offer a basis for understanding P. acnes virulent properties, host adaptation mechanisms, and its potential role in acne pathogenesis at the strain level. Furthermore, the plasmid identified in HL096PA1 may potentially provide a new opportunity for P. acnes genetic manipulation and targeted therapy against specific disease-associated strains.http://dx.doi.org/10.1155/2013/918320 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gabriela Kasimatis Sorel Fitz-Gibbon Shuta Tomida Marthew Wong Huiying Li |
spellingShingle |
Gabriela Kasimatis Sorel Fitz-Gibbon Shuta Tomida Marthew Wong Huiying Li Analysis of Complete Genomes of Propionibacterium acnes Reveals a Novel Plasmid and Increased Pseudogenes in an Acne Associated Strain BioMed Research International |
author_facet |
Gabriela Kasimatis Sorel Fitz-Gibbon Shuta Tomida Marthew Wong Huiying Li |
author_sort |
Gabriela Kasimatis |
title |
Analysis of Complete Genomes of Propionibacterium acnes Reveals a Novel Plasmid and Increased Pseudogenes in an Acne Associated Strain |
title_short |
Analysis of Complete Genomes of Propionibacterium acnes Reveals a Novel Plasmid and Increased Pseudogenes in an Acne Associated Strain |
title_full |
Analysis of Complete Genomes of Propionibacterium acnes Reveals a Novel Plasmid and Increased Pseudogenes in an Acne Associated Strain |
title_fullStr |
Analysis of Complete Genomes of Propionibacterium acnes Reveals a Novel Plasmid and Increased Pseudogenes in an Acne Associated Strain |
title_full_unstemmed |
Analysis of Complete Genomes of Propionibacterium acnes Reveals a Novel Plasmid and Increased Pseudogenes in an Acne Associated Strain |
title_sort |
analysis of complete genomes of propionibacterium acnes reveals a novel plasmid and increased pseudogenes in an acne associated strain |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2013-01-01 |
description |
The human skin harbors a diverse community of bacteria, including the Gram-positive, anaerobic bacterium Propionibacterium acnes. P. acnes has historically been linked to the pathogenesis of acne vulgaris, a common skin disease affecting over 80% of all adolescents in the US. To gain insight into potential P. acnes pathogenic mechanisms, we previously sequenced the complete genome of a P. acnes strain HL096PA1 that is highly associated with acne. In this study, we compared its genome to the first published complete genome KPA171202. HL096PA1 harbors a linear plasmid, pIMPLE-HL096PA1. This is the first described P. acnes plasmid. We also observed a five-fold increase of pseudogenes in HL096PA1, several of which encode proteins in carbohydrate transport and metabolism. In addition, our analysis revealed a few island-like genomic regions that are unique to HL096PA1 and a large genomic inversion spanning the ribosomal operons. Together, these findings offer a basis for understanding P. acnes virulent properties, host adaptation mechanisms, and its potential role in acne pathogenesis at the strain level. Furthermore, the plasmid identified in HL096PA1 may potentially provide a new opportunity for P. acnes genetic manipulation and targeted therapy against specific disease-associated strains. |
url |
http://dx.doi.org/10.1155/2013/918320 |
work_keys_str_mv |
AT gabrielakasimatis analysisofcompletegenomesofpropionibacteriumacnesrevealsanovelplasmidandincreasedpseudogenesinanacneassociatedstrain AT sorelfitzgibbon analysisofcompletegenomesofpropionibacteriumacnesrevealsanovelplasmidandincreasedpseudogenesinanacneassociatedstrain AT shutatomida analysisofcompletegenomesofpropionibacteriumacnesrevealsanovelplasmidandincreasedpseudogenesinanacneassociatedstrain AT marthewwong analysisofcompletegenomesofpropionibacteriumacnesrevealsanovelplasmidandincreasedpseudogenesinanacneassociatedstrain AT huiyingli analysisofcompletegenomesofpropionibacteriumacnesrevealsanovelplasmidandincreasedpseudogenesinanacneassociatedstrain |
_version_ |
1725740057691684864 |