In Vitro Effects of Pumpkin (Cucurbita moschata) Seed Extracts on Echinococcus granulosus Protoscoleces

• Main Authors: Zahra HESARI, Meysam SHARIFDINI, Mohammad Kazem SHARIFI-YAZDI, Saeedeh GHAFARI, Sara GHASEMI, Shahram MAHMOUDI, Mehdi MOHEBALI, Bahram NIKMANESH
• Format: Article
• Language: English
• Published: Tehran University of Medical Sciences 2020-03-01
• Series: Iranian Journal of Parasitology
• Subjects: Cucurbita moschata; Pumpkin seed extract; Echinococcus granulosus; Scolicidal effect
• Online Access: https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2838

Background: Echinococcus granulosus parasite causes a zoonotic disease which is important for public and veterinary health. Since pumpkin seeds (Cucurbita sp.) are used as traditional vermifuge in Iran, they may be a potential herbal anthelmintic. Methods: This study was designed in 2016 to evaluate the in vitro scolicidal effect of Cucurbita moschata seeds form northern part of Iran. Hydroalcoholic and petroleum ether extracts were prepared by maceration and soxhlet respectively. Both extracts with four different concentrations (100, 10, 1, 0.1 mg/ml) were incubated against protoscoleces in 5, 15, 30 and 60 min. Results: Maximum mortality was 16% with 1% hydroalcoholic extract in 60 min. The highest mortality with organic extract was 4% with 10% concentration in 60 min (P=0.015). Conclusion: Since highest mortality was 16%, the extract did not reach to LD50 (50% mortality). Therefore, the potency of the total extract is not sufficient as potential scolicidal drug.