Searching for convergent evolution in manganese superoxidase dismutase using hydrophobic cluster analysis
There are numerous examples of convergent evolution in nature. Major ecological adaptations such as flight, loss of limbs in vertebrates, pesticide resistance, adaptation to a parasitic way of life, etc., have all evolved more than once, as seen by their analogous functions in separate taxa. But wha...
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2014-06-01
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doaj-4d98ed8db0014df5823f82ef15f2ed6f2020-11-24T21:11:04ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-46852014-06-0137246047010.1590/S1415-47572014000300019S1415-47572014000300019Searching for convergent evolution in manganese superoxidase dismutase using hydrophobic cluster analysisHeng Xiang0Ruizhi Zhang1Nengzhang Li2Charles R. Vossbrinck3Southwest UniversitySouthwest UniversitySouthwest UniversityConnecticut Agricultural Experiment StationThere are numerous examples of convergent evolution in nature. Major ecological adaptations such as flight, loss of limbs in vertebrates, pesticide resistance, adaptation to a parasitic way of life, etc., have all evolved more than once, as seen by their analogous functions in separate taxa. But what about protein evolution? Does the environment have a strong enough influence on intracellular processes that enzymes and other functional proteins play, to evolve similar functional roles separately in different organisms? Manganese Superoxide Dismutase (MnSOD) is a manganesedependant metallo-enzyme which plays a crucial role in protecting cells from anti-oxidative stress by eliminating reactive (superoxide) oxygen species. It is a ubiquitous housekeeping enzyme found in nearly all organisms. In this study we compare phylogenies based on MnSOD protein sequences to those based on scores from Hydrophobic Cluster Analysis (HCA). We calculated HCA similarity values for each pair of taxa to obtain a pair-wise distance matrix. A UPGMA tree based on the HCA distance matrix and a common tree based on the primary protein sequence for MnSOD was constructed. Differences between these two trees within animals, enterobacteriaceae, planctomycetes and cyanobacteria are presented and cited as possible examples of convergence. We note that several residue changes result in changes in hydrophobicity at positions which apparently are under the effect of positive selection.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300019&lng=en&tlng=enconvergencehydrophobic cluster analysismanganese superoxide dismutasephylogenystructure |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Heng Xiang Ruizhi Zhang Nengzhang Li Charles R. Vossbrinck |
spellingShingle |
Heng Xiang Ruizhi Zhang Nengzhang Li Charles R. Vossbrinck Searching for convergent evolution in manganese superoxidase dismutase using hydrophobic cluster analysis Genetics and Molecular Biology convergence hydrophobic cluster analysis manganese superoxide dismutase phylogeny structure |
author_facet |
Heng Xiang Ruizhi Zhang Nengzhang Li Charles R. Vossbrinck |
author_sort |
Heng Xiang |
title |
Searching for convergent evolution in manganese superoxidase dismutase using hydrophobic cluster analysis |
title_short |
Searching for convergent evolution in manganese superoxidase dismutase using hydrophobic cluster analysis |
title_full |
Searching for convergent evolution in manganese superoxidase dismutase using hydrophobic cluster analysis |
title_fullStr |
Searching for convergent evolution in manganese superoxidase dismutase using hydrophobic cluster analysis |
title_full_unstemmed |
Searching for convergent evolution in manganese superoxidase dismutase using hydrophobic cluster analysis |
title_sort |
searching for convergent evolution in manganese superoxidase dismutase using hydrophobic cluster analysis |
publisher |
Sociedade Brasileira de Genética |
series |
Genetics and Molecular Biology |
issn |
1678-4685 |
publishDate |
2014-06-01 |
description |
There are numerous examples of convergent evolution in nature. Major ecological adaptations such as flight, loss of limbs in vertebrates, pesticide resistance, adaptation to a parasitic way of life, etc., have all evolved more than once, as seen by their analogous functions in separate taxa. But what about protein evolution? Does the environment have a strong enough influence on intracellular processes that enzymes and other functional proteins play, to evolve similar functional roles separately in different organisms? Manganese Superoxide Dismutase (MnSOD) is a manganesedependant metallo-enzyme which plays a crucial role in protecting cells from anti-oxidative stress by eliminating reactive (superoxide) oxygen species. It is a ubiquitous housekeeping enzyme found in nearly all organisms. In this study we compare phylogenies based on MnSOD protein sequences to those based on scores from Hydrophobic Cluster Analysis (HCA). We calculated HCA similarity values for each pair of taxa to obtain a pair-wise distance matrix. A UPGMA tree based on the HCA distance matrix and a common tree based on the primary protein sequence for MnSOD was constructed. Differences between these two trees within animals, enterobacteriaceae, planctomycetes and cyanobacteria are presented and cited as possible examples of convergence. We note that several residue changes result in changes in hydrophobicity at positions which apparently are under the effect of positive selection. |
topic |
convergence hydrophobic cluster analysis manganese superoxide dismutase phylogeny structure |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300019&lng=en&tlng=en |
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