Is <i>Lactobacillus</i> Gram-Positive? A Case Study of <i>Lactobacillus iners</i>

• Main Authors: Hyaekang Kim, Taehyun Kim, Jaeku Kang, Younghoon Kim, Heebal Kim
• Format: Article
• Language: English
• Published: MDPI AG 2020-06-01
• Series: Microorganisms
• Subjects: Lactobacillus iners; TEM; peptidoglycan; gram-negative
• Online Access: https://www.mdpi.com/2076-2607/8/7/969

<i>Lactobacillus iners</i> is the most prevalent bacterial species in the human vaginal microbiome, and there have been few reports of its Gram-negative stain appearances despite the fact that the genus <i>Lactobacillus </i>is universally described as Gram-positive. Here, using transmission electron microscopy, we reveal that the thinness of the cell wall (17.39 ± 0.8 nm) gives the Gram-negative stain appearance, which can lead to over-diagnosis of bacterial vaginosis. Moreover, comparative genome analysis identified four genes commonly absent in <i>L.</i> <i>iners</i> genomes that might contribute to this phenotypic difference. We suggest that, along with the several niche-specific attributes identified, this unique feature may contribute to the species’ distinguished capability to thrive as the predominant species in the fluctuating vaginal environment as well.