Effects of PEDF on the expression of MCP-1 in mice retina of oxygen-induced retinopathy
AIM: To observe the effect of pigment epithelium-derived factor(PEDF)on retinal neovascularization(RNV)and monocyte chemoattractant protein-1(MCP-1)expressions in mice retina of oxygen-induced retinopathy(OIR), and to investigate the protective effect of PEDF on ischemia hypoxia retinopathy and the...
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doaj-4da04415cd01418493bc44870a8770192020-11-24T21:51:53ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232019-01-01191212510.3980/j.issn.1672-5123.2019.1.04Effects of PEDF on the expression of MCP-1 in mice retina of oxygen-induced retinopathyYa-Nuo Wang0Lei Zhang1Department of Ophthalmology, Shanghai Seventh People's Hospital of Shanghai University of TCM, Shanghai 200137, ChinaDepartment of Ophthalmology, Shanghai Seventh People's Hospital of Shanghai University of TCM, Shanghai 200137, ChinaAIM: To observe the effect of pigment epithelium-derived factor(PEDF)on retinal neovascularization(RNV)and monocyte chemoattractant protein-1(MCP-1)expressions in mice retina of oxygen-induced retinopathy(OIR), and to investigate the protective effect of PEDF on ischemia hypoxia retinopathy and the possible mechanism. <p>METHODS: A total of 160 postnatal day(P)7 C57BL/6 mice were obtained. All mice except normal control group were exposed to(75±2)% oxygen environment for 5d and then kept in room air for another 5d to establish the OIR mice model. All mice in normal control group(40 mice)were exposed to room air only. At P12 and P14, respectively, mice in PEDF treatment group were injected intravitreously with recombinant human PEDF(2μg/eye,1μL)in the right eye, while mice in treatment control group were injected intravitreously with the same volume of vehicle [1μL, 10mmol/L phosphate buffered saline(PH7.4), PBS] in the right eye. All mice were euthanized at P17. Eyes were whole mounted and stained with Lectin to observe the growth of abnormal RNV; And retinal specimens were prepared for PEDF, MCP-1 protein and mRNA analysis by Western blot and real time RT-PCR respectively. <p>RESULTS: Changes of retinal vessels had been detected by fluorescence microscopy on flat-mounted retina. The relative RNV areas were significantly increased in OIR model group compared with those in normal control group(<i>P</i><0.01). However, the relative RNV areas were significantly reduced in PEDF treatment group compared with those in PBS treatment control group(<i>P</i><0.01). The specific expression of MCP-1 protein and mRNA in the OIR model group were higher than those of normal control group, presenting a statistically significance(both <i>P</i><0.05). The specific expression of PEDF protein and mRNA in the OIR model group showed a considerable decline in comparison with normal control group, presenting a statistically significance(both <i>P</i><0.01). And the specific expression of MCP-1 protein and mRNA in those of PEDF-treated group showed a considerable decline in comparison with PBS-treated group, and the differences were statistically significant(both <i>P</i><0.05). However, there were increase of the expression of MCP-1 protein and mRNA between normal control group and PEDF-treated group, presenting no statistically significance(both <i>P</i>>0.05). <p>CONCLUSION: PEDF could inhibit oxygen-induced retinal neovascularization and down-regulate retinal MCP-1 expression under hypoxia, which may underlie its anti-neovascularization effects and play a role of protection in ischemic retinopathy.http://ies.ijo.cn/cn_publish/2019/1/201901004.pdfoxygen-induced retinopathypigment epithelium derived factormonocyte chemoattractant protein-1retinal neovascularization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ya-Nuo Wang Lei Zhang |
spellingShingle |
Ya-Nuo Wang Lei Zhang Effects of PEDF on the expression of MCP-1 in mice retina of oxygen-induced retinopathy Guoji Yanke Zazhi oxygen-induced retinopathy pigment epithelium derived factor monocyte chemoattractant protein-1 retinal neovascularization |
author_facet |
Ya-Nuo Wang Lei Zhang |
author_sort |
Ya-Nuo Wang |
title |
Effects of PEDF on the expression of MCP-1 in mice retina of oxygen-induced retinopathy |
title_short |
Effects of PEDF on the expression of MCP-1 in mice retina of oxygen-induced retinopathy |
title_full |
Effects of PEDF on the expression of MCP-1 in mice retina of oxygen-induced retinopathy |
title_fullStr |
Effects of PEDF on the expression of MCP-1 in mice retina of oxygen-induced retinopathy |
title_full_unstemmed |
Effects of PEDF on the expression of MCP-1 in mice retina of oxygen-induced retinopathy |
title_sort |
effects of pedf on the expression of mcp-1 in mice retina of oxygen-induced retinopathy |
publisher |
Press of International Journal of Ophthalmology (IJO PRESS) |
series |
Guoji Yanke Zazhi |
issn |
1672-5123 1672-5123 |
publishDate |
2019-01-01 |
description |
AIM: To observe the effect of pigment epithelium-derived factor(PEDF)on retinal neovascularization(RNV)and monocyte chemoattractant protein-1(MCP-1)expressions in mice retina of oxygen-induced retinopathy(OIR), and to investigate the protective effect of PEDF on ischemia hypoxia retinopathy and the possible mechanism. <p>METHODS: A total of 160 postnatal day(P)7 C57BL/6 mice were obtained. All mice except normal control group were exposed to(75±2)% oxygen environment for 5d and then kept in room air for another 5d to establish the OIR mice model. All mice in normal control group(40 mice)were exposed to room air only. At P12 and P14, respectively, mice in PEDF treatment group were injected intravitreously with recombinant human PEDF(2μg/eye,1μL)in the right eye, while mice in treatment control group were injected intravitreously with the same volume of vehicle [1μL, 10mmol/L phosphate buffered saline(PH7.4), PBS] in the right eye. All mice were euthanized at P17. Eyes were whole mounted and stained with Lectin to observe the growth of abnormal RNV; And retinal specimens were prepared for PEDF, MCP-1 protein and mRNA analysis by Western blot and real time RT-PCR respectively. <p>RESULTS: Changes of retinal vessels had been detected by fluorescence microscopy on flat-mounted retina. The relative RNV areas were significantly increased in OIR model group compared with those in normal control group(<i>P</i><0.01). However, the relative RNV areas were significantly reduced in PEDF treatment group compared with those in PBS treatment control group(<i>P</i><0.01). The specific expression of MCP-1 protein and mRNA in the OIR model group were higher than those of normal control group, presenting a statistically significance(both <i>P</i><0.05). The specific expression of PEDF protein and mRNA in the OIR model group showed a considerable decline in comparison with normal control group, presenting a statistically significance(both <i>P</i><0.01). And the specific expression of MCP-1 protein and mRNA in those of PEDF-treated group showed a considerable decline in comparison with PBS-treated group, and the differences were statistically significant(both <i>P</i><0.05). However, there were increase of the expression of MCP-1 protein and mRNA between normal control group and PEDF-treated group, presenting no statistically significance(both <i>P</i>>0.05). <p>CONCLUSION: PEDF could inhibit oxygen-induced retinal neovascularization and down-regulate retinal MCP-1 expression under hypoxia, which may underlie its anti-neovascularization effects and play a role of protection in ischemic retinopathy. |
topic |
oxygen-induced retinopathy pigment epithelium derived factor monocyte chemoattractant protein-1 retinal neovascularization |
url |
http://ies.ijo.cn/cn_publish/2019/1/201901004.pdf |
work_keys_str_mv |
AT yanuowang effectsofpedfontheexpressionofmcp1inmiceretinaofoxygeninducedretinopathy AT leizhang effectsofpedfontheexpressionofmcp1inmiceretinaofoxygeninducedretinopathy |
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