St. John’s Wort Suppresses Growth in Triple-Negative Breast Cancer Cell Line MDA-MB-231 by Inducing Prodeath Autophagy and Apoptosis
The rational regulation of programmed cell death by means of autophagy and apoptosis has been considered a potential treatment strategy for cancer. We demonstrated the inhibitory effect of St. John’s Wort (SJW) on growth in the triple-negative breast cancer (TNBC) cell line and xenografted mice and...
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MDPI AG
2020-10-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/10/3175 |
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doaj-4da9783b52554f899af47dcd30934d7e2020-11-25T03:41:41ZengMDPI AGNutrients2072-66432020-10-01123175317510.3390/nu12103175St. John’s Wort Suppresses Growth in Triple-Negative Breast Cancer Cell Line MDA-MB-231 by Inducing Prodeath Autophagy and ApoptosisMikyoung You0Young-Hyun Lee1Hwa-Jin Kim2Ji Hyun Kook3Hyeon-A Kim4Department of Nutrition, University of Massachusetts Amherst, Amherst, MA 01003, USADepartment of Food and Nutrition, Mokpo National University, Jeollanam-do 58554, KoreaDepartment of Food and Nutrition, Mokpo National University, Jeollanam-do 58554, KoreaDepartment of Food and Nutrition, Mokpo National University, Jeollanam-do 58554, KoreaDepartment of Food and Nutrition, Mokpo National University, Jeollanam-do 58554, KoreaThe rational regulation of programmed cell death by means of autophagy and apoptosis has been considered a potential treatment strategy for cancer. We demonstrated the inhibitory effect of St. John’s Wort (SJW) on growth in the triple-negative breast cancer (TNBC) cell line and xenografted mice and its target mechanism concerning autophagic and apoptotic cell death. SJW ethanol extract (SJWE) inhibited proliferation in a dose-dependent manner. SJWE treatment dramatically increased autophagy flux and apoptosis compared with the control. The autophagy inhibitor, 3-methyladenine (3-MA), reversed the SJWE-induced inhibition of cell proliferation and regulation of autophagy and apoptosis, indicating that SJWE induced apoptosis through prodeath autophagy. Furthermore, SJWE inhibited tumor growth and induced autophagy and apoptosis in the tumor of MDA-MB-231 xenografted athymic nude mice. Our results indicate that SJWE might have great potential as a new anticancer therapy for triple-negative breast cancer by inducing prodeath autophagy and apoptosis.https://www.mdpi.com/2072-6643/12/10/3175triple-negative breast cancerautophagyapoptosisSt. John’s Wort |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Mikyoung You Young-Hyun Lee Hwa-Jin Kim Ji Hyun Kook Hyeon-A Kim |
| spellingShingle |
Mikyoung You Young-Hyun Lee Hwa-Jin Kim Ji Hyun Kook Hyeon-A Kim St. John’s Wort Suppresses Growth in Triple-Negative Breast Cancer Cell Line MDA-MB-231 by Inducing Prodeath Autophagy and Apoptosis Nutrients triple-negative breast cancer autophagy apoptosis St. John’s Wort |
| author_facet |
Mikyoung You Young-Hyun Lee Hwa-Jin Kim Ji Hyun Kook Hyeon-A Kim |
| author_sort |
Mikyoung You |
| title |
St. John’s Wort Suppresses Growth in Triple-Negative Breast Cancer Cell Line MDA-MB-231 by Inducing Prodeath Autophagy and Apoptosis |
| title_short |
St. John’s Wort Suppresses Growth in Triple-Negative Breast Cancer Cell Line MDA-MB-231 by Inducing Prodeath Autophagy and Apoptosis |
| title_full |
St. John’s Wort Suppresses Growth in Triple-Negative Breast Cancer Cell Line MDA-MB-231 by Inducing Prodeath Autophagy and Apoptosis |
| title_fullStr |
St. John’s Wort Suppresses Growth in Triple-Negative Breast Cancer Cell Line MDA-MB-231 by Inducing Prodeath Autophagy and Apoptosis |
| title_full_unstemmed |
St. John’s Wort Suppresses Growth in Triple-Negative Breast Cancer Cell Line MDA-MB-231 by Inducing Prodeath Autophagy and Apoptosis |
| title_sort |
st. john’s wort suppresses growth in triple-negative breast cancer cell line mda-mb-231 by inducing prodeath autophagy and apoptosis |
| publisher |
MDPI AG |
| series |
Nutrients |
| issn |
2072-6643 |
| publishDate |
2020-10-01 |
| description |
The rational regulation of programmed cell death by means of autophagy and apoptosis has been considered a potential treatment strategy for cancer. We demonstrated the inhibitory effect of St. John’s Wort (SJW) on growth in the triple-negative breast cancer (TNBC) cell line and xenografted mice and its target mechanism concerning autophagic and apoptotic cell death. SJW ethanol extract (SJWE) inhibited proliferation in a dose-dependent manner. SJWE treatment dramatically increased autophagy flux and apoptosis compared with the control. The autophagy inhibitor, 3-methyladenine (3-MA), reversed the SJWE-induced inhibition of cell proliferation and regulation of autophagy and apoptosis, indicating that SJWE induced apoptosis through prodeath autophagy. Furthermore, SJWE inhibited tumor growth and induced autophagy and apoptosis in the tumor of MDA-MB-231 xenografted athymic nude mice. Our results indicate that SJWE might have great potential as a new anticancer therapy for triple-negative breast cancer by inducing prodeath autophagy and apoptosis. |
| topic |
triple-negative breast cancer autophagy apoptosis St. John’s Wort |
| url |
https://www.mdpi.com/2072-6643/12/10/3175 |
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