Pathogenicity Analysis of Weaned Piglets Challenged With Novel Emerging Senecavirus A in Fujian, China
In order to evaluate the pathogenicity of Senecavirus A (SVA) to weaned piglets preliminarily, 28-day-old weaned piglets were challenged with SVA by intramuscular injection. The clinical manifestations, antibody levels, and tissue viral load of infected piglets were detected. The results indicated t...
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doaj-4daf70f560144f8d84bf5319058894e72021-07-07T05:21:35ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-07-01810.3389/fvets.2021.694110694110Pathogenicity Analysis of Weaned Piglets Challenged With Novel Emerging Senecavirus A in Fujian, ChinaCun Liu0Cun Liu1Yanhan Liu2Yanhan Liu3Xiubo Li4Xiubo Li5Lin Liang6Lin Liang7Shangjin Cui8Shangjin Cui9Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaShandong Provincial Center for Animal Disease Control, Ji'nan, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaShandong Provincial Center for Animal Disease Control, Ji'nan, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaBeijing Observation Station for Veterinary Drug and Veterinary Biotechnology, Ministry of Agriculture, Beijing, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaBeijing Observation Station for Veterinary Drug and Veterinary Biotechnology, Ministry of Agriculture, Beijing, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaBeijing Observation Station for Veterinary Drug and Veterinary Biotechnology, Ministry of Agriculture, Beijing, ChinaIn order to evaluate the pathogenicity of Senecavirus A (SVA) to weaned piglets preliminarily, 28-day-old weaned piglets were challenged with SVA by intramuscular injection. The clinical manifestations, antibody levels, and tissue viral load of infected piglets were detected. The results indicated that the piglets challenged with SVA CH/FuJ/2017 showed drowsiness, lameness, oral blisters, diarrhea, and other clinical signs. Lesions on the hooves were observed. Red spots or plaques were initially observed on the hoof and then developed into blisters that cracked and gradually formed scab. The symptoms and signs were relieved after 8 days post-infection (dpi). The sentinel piglet, feeding together with the challenged piglets, showed similar clinical signs with the challenged piglets after 3 dpi. Monitoring of antibody levels showed that anti-SVA antibody could be detected at 5 dpi by competition enzyme-linked immunosorbent assay (cELISA) method, and neutralizing antibody could be detected after 7 dpi. Analysis of viral tissue distribution and viral load indicated that SVA could replicate in the liver, spleen, lung, kidney, and lymph node. In all, Senecavirus disease was successfully replicated by SVA CH/FuJ/2017 isolate, which verified the clinical manifestations of SVA infection in weaned piglets, and provided a foundation for further SVA pathogenesis and vaccine development.https://www.frontiersin.org/articles/10.3389/fvets.2021.694110/fullemerging diseasepathogenicityvesicular diseaseSenecavirus Aweaned piglets |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cun Liu Cun Liu Yanhan Liu Yanhan Liu Xiubo Li Xiubo Li Lin Liang Lin Liang Shangjin Cui Shangjin Cui |
spellingShingle |
Cun Liu Cun Liu Yanhan Liu Yanhan Liu Xiubo Li Xiubo Li Lin Liang Lin Liang Shangjin Cui Shangjin Cui Pathogenicity Analysis of Weaned Piglets Challenged With Novel Emerging Senecavirus A in Fujian, China Frontiers in Veterinary Science emerging disease pathogenicity vesicular disease Senecavirus A weaned piglets |
author_facet |
Cun Liu Cun Liu Yanhan Liu Yanhan Liu Xiubo Li Xiubo Li Lin Liang Lin Liang Shangjin Cui Shangjin Cui |
author_sort |
Cun Liu |
title |
Pathogenicity Analysis of Weaned Piglets Challenged With Novel Emerging Senecavirus A in Fujian, China |
title_short |
Pathogenicity Analysis of Weaned Piglets Challenged With Novel Emerging Senecavirus A in Fujian, China |
title_full |
Pathogenicity Analysis of Weaned Piglets Challenged With Novel Emerging Senecavirus A in Fujian, China |
title_fullStr |
Pathogenicity Analysis of Weaned Piglets Challenged With Novel Emerging Senecavirus A in Fujian, China |
title_full_unstemmed |
Pathogenicity Analysis of Weaned Piglets Challenged With Novel Emerging Senecavirus A in Fujian, China |
title_sort |
pathogenicity analysis of weaned piglets challenged with novel emerging senecavirus a in fujian, china |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Veterinary Science |
issn |
2297-1769 |
publishDate |
2021-07-01 |
description |
In order to evaluate the pathogenicity of Senecavirus A (SVA) to weaned piglets preliminarily, 28-day-old weaned piglets were challenged with SVA by intramuscular injection. The clinical manifestations, antibody levels, and tissue viral load of infected piglets were detected. The results indicated that the piglets challenged with SVA CH/FuJ/2017 showed drowsiness, lameness, oral blisters, diarrhea, and other clinical signs. Lesions on the hooves were observed. Red spots or plaques were initially observed on the hoof and then developed into blisters that cracked and gradually formed scab. The symptoms and signs were relieved after 8 days post-infection (dpi). The sentinel piglet, feeding together with the challenged piglets, showed similar clinical signs with the challenged piglets after 3 dpi. Monitoring of antibody levels showed that anti-SVA antibody could be detected at 5 dpi by competition enzyme-linked immunosorbent assay (cELISA) method, and neutralizing antibody could be detected after 7 dpi. Analysis of viral tissue distribution and viral load indicated that SVA could replicate in the liver, spleen, lung, kidney, and lymph node. In all, Senecavirus disease was successfully replicated by SVA CH/FuJ/2017 isolate, which verified the clinical manifestations of SVA infection in weaned piglets, and provided a foundation for further SVA pathogenesis and vaccine development. |
topic |
emerging disease pathogenicity vesicular disease Senecavirus A weaned piglets |
url |
https://www.frontiersin.org/articles/10.3389/fvets.2021.694110/full |
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