In vivo calcium imaging from dentate granule cells with wide-field fluorescence microscopy.
A combination of genetically-encoded calcium indicators and micro-optics has enabled monitoring of large-scale dynamics of neuronal activity from behaving animals. In these studies, wide-field microscopy is often used to visualize neural activity. However, this method lacks optical sectioning capabi...
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doaj-4db02a4b2db24ab4b2f88afed917a9632020-11-25T01:32:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01127e018045210.1371/journal.pone.0180452In vivo calcium imaging from dentate granule cells with wide-field fluorescence microscopy.Yuichiro HayashiSatoshi YawataKazuo FunabikiTakatoshi HikidaA combination of genetically-encoded calcium indicators and micro-optics has enabled monitoring of large-scale dynamics of neuronal activity from behaving animals. In these studies, wide-field microscopy is often used to visualize neural activity. However, this method lacks optical sectioning capability, and therefore its axial resolution is generally poor. At present, it is unclear whether wide-field microscopy can visualize activity of densely packed small neurons at cellular resolution. To examine the applicability of wide-field microscopy for small-sized neurons, we recorded calcium activity of dentate granule cells having a small soma diameter of approximately 10 micrometers. Using a combination of high numerical aperture (0.8) objective lens and independent component analysis-based image segmentation technique, activity of putative single granule cell activity was separated from wide-field calcium imaging data. The result encourages wider application of wide-field microscopy in in vivo neurophysiology.http://europepmc.org/articles/PMC5507494?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuichiro Hayashi Satoshi Yawata Kazuo Funabiki Takatoshi Hikida |
spellingShingle |
Yuichiro Hayashi Satoshi Yawata Kazuo Funabiki Takatoshi Hikida In vivo calcium imaging from dentate granule cells with wide-field fluorescence microscopy. PLoS ONE |
author_facet |
Yuichiro Hayashi Satoshi Yawata Kazuo Funabiki Takatoshi Hikida |
author_sort |
Yuichiro Hayashi |
title |
In vivo calcium imaging from dentate granule cells with wide-field fluorescence microscopy. |
title_short |
In vivo calcium imaging from dentate granule cells with wide-field fluorescence microscopy. |
title_full |
In vivo calcium imaging from dentate granule cells with wide-field fluorescence microscopy. |
title_fullStr |
In vivo calcium imaging from dentate granule cells with wide-field fluorescence microscopy. |
title_full_unstemmed |
In vivo calcium imaging from dentate granule cells with wide-field fluorescence microscopy. |
title_sort |
in vivo calcium imaging from dentate granule cells with wide-field fluorescence microscopy. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2017-01-01 |
description |
A combination of genetically-encoded calcium indicators and micro-optics has enabled monitoring of large-scale dynamics of neuronal activity from behaving animals. In these studies, wide-field microscopy is often used to visualize neural activity. However, this method lacks optical sectioning capability, and therefore its axial resolution is generally poor. At present, it is unclear whether wide-field microscopy can visualize activity of densely packed small neurons at cellular resolution. To examine the applicability of wide-field microscopy for small-sized neurons, we recorded calcium activity of dentate granule cells having a small soma diameter of approximately 10 micrometers. Using a combination of high numerical aperture (0.8) objective lens and independent component analysis-based image segmentation technique, activity of putative single granule cell activity was separated from wide-field calcium imaging data. The result encourages wider application of wide-field microscopy in in vivo neurophysiology. |
url |
http://europepmc.org/articles/PMC5507494?pdf=render |
work_keys_str_mv |
AT yuichirohayashi invivocalciumimagingfromdentategranulecellswithwidefieldfluorescencemicroscopy AT satoshiyawata invivocalciumimagingfromdentategranulecellswithwidefieldfluorescencemicroscopy AT kazuofunabiki invivocalciumimagingfromdentategranulecellswithwidefieldfluorescencemicroscopy AT takatoshihikida invivocalciumimagingfromdentategranulecellswithwidefieldfluorescencemicroscopy |
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