IL-37 Expression Reduces Lean Body Mass in Mice by Reducing Food Intake

The human cytokine interleukin (IL)-37 is an anti-inflammatory member of the IL-1 family of cytokines. Transgenic expression of IL-37 in mice protects them from diet-induced obesity and associated metabolic complications including dyslipidemia, inflammation and insulin resistance. The precise mechan...

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Main Authors: Eline N. Kuipers, Andrea D. van Dam, Dov B. Ballak, Ellemiek A. de Wit, Charles A. Dinarello, Rinke Stienstra, Janna A. van Diepen, Patrick C.N. Rensen, Mariëtte R. Boon
Format: Article
Language:English
Published: MDPI AG 2018-08-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:http://www.mdpi.com/1422-0067/19/8/2264
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spelling doaj-4dbb2c7596974308ac867267ee07ce912020-11-24T21:06:13ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-08-01198226410.3390/ijms19082264ijms19082264IL-37 Expression Reduces Lean Body Mass in Mice by Reducing Food IntakeEline N. Kuipers0Andrea D. van Dam1Dov B. Ballak2Ellemiek A. de Wit3Charles A. Dinarello4Rinke Stienstra5Janna A. van Diepen6Patrick C.N. Rensen7Mariëtte R. Boon8Department of Medicine, Division of Endocrinology, Leiden University Medical Center, Post Zone C7Q, P.O. Box 9600, 2300 RC Leiden, The NetherlandsDepartment of Medicine, Division of Endocrinology, Leiden University Medical Center, Post Zone C7Q, P.O. Box 9600, 2300 RC Leiden, The NetherlandsDepartment of Medicine, University of Colorado, Aurora, CO 80045, USADepartment of Medicine, Division of Endocrinology, Leiden University Medical Center, Post Zone C7Q, P.O. Box 9600, 2300 RC Leiden, The NetherlandsDepartment of Medicine, University of Colorado, Aurora, CO 80045, USADepartment of General Internal Medicine and Radboud Center for Infectious Diseases, Radboud University Nijmegen Medical Center, 6525 HP Nijmegen, The NetherlandsDepartment of General Internal Medicine and Radboud Center for Infectious Diseases, Radboud University Nijmegen Medical Center, 6525 HP Nijmegen, The NetherlandsDepartment of Medicine, Division of Endocrinology, Leiden University Medical Center, Post Zone C7Q, P.O. Box 9600, 2300 RC Leiden, The NetherlandsDepartment of Medicine, Division of Endocrinology, Leiden University Medical Center, Post Zone C7Q, P.O. Box 9600, 2300 RC Leiden, The NetherlandsThe human cytokine interleukin (IL)-37 is an anti-inflammatory member of the IL-1 family of cytokines. Transgenic expression of IL-37 in mice protects them from diet-induced obesity and associated metabolic complications including dyslipidemia, inflammation and insulin resistance. The precise mechanism of action leading to these beneficial metabolic effects is not entirely known. Therefore, we aimed to assess in detail the effect of transgenic IL-37 expression on energy balance, including food intake and energy expenditure. Feeding homozygous IL-37 transgenic mice and wild-type (WT) control mice a high-fat diet (HFD; 45% kcal palm fat) for 6 weeks showed that IL-37 reduced body weight related to a marked decrease in food intake. Subsequent mechanistic studies in mice with heterozygous IL-37 expression versus WT littermates, fed the HFD for 18 weeks, confirmed that IL-37 reduces food intake, which led to a decrease in lean body mass, but did not reduce fat mass and plasma lipid levels or alterations in energy expenditure independent of lean body mass. Taken together, this suggests that IL-37 reduces lean body mass by reducing food intake.http://www.mdpi.com/1422-0067/19/8/2264IL-37energy metabolismfood intakehigh fat diet
collection DOAJ
language English
format Article
sources DOAJ
author Eline N. Kuipers
Andrea D. van Dam
Dov B. Ballak
Ellemiek A. de Wit
Charles A. Dinarello
Rinke Stienstra
Janna A. van Diepen
Patrick C.N. Rensen
Mariëtte R. Boon
spellingShingle Eline N. Kuipers
Andrea D. van Dam
Dov B. Ballak
Ellemiek A. de Wit
Charles A. Dinarello
Rinke Stienstra
Janna A. van Diepen
Patrick C.N. Rensen
Mariëtte R. Boon
IL-37 Expression Reduces Lean Body Mass in Mice by Reducing Food Intake
International Journal of Molecular Sciences
IL-37
energy metabolism
food intake
high fat diet
author_facet Eline N. Kuipers
Andrea D. van Dam
Dov B. Ballak
Ellemiek A. de Wit
Charles A. Dinarello
Rinke Stienstra
Janna A. van Diepen
Patrick C.N. Rensen
Mariëtte R. Boon
author_sort Eline N. Kuipers
title IL-37 Expression Reduces Lean Body Mass in Mice by Reducing Food Intake
title_short IL-37 Expression Reduces Lean Body Mass in Mice by Reducing Food Intake
title_full IL-37 Expression Reduces Lean Body Mass in Mice by Reducing Food Intake
title_fullStr IL-37 Expression Reduces Lean Body Mass in Mice by Reducing Food Intake
title_full_unstemmed IL-37 Expression Reduces Lean Body Mass in Mice by Reducing Food Intake
title_sort il-37 expression reduces lean body mass in mice by reducing food intake
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2018-08-01
description The human cytokine interleukin (IL)-37 is an anti-inflammatory member of the IL-1 family of cytokines. Transgenic expression of IL-37 in mice protects them from diet-induced obesity and associated metabolic complications including dyslipidemia, inflammation and insulin resistance. The precise mechanism of action leading to these beneficial metabolic effects is not entirely known. Therefore, we aimed to assess in detail the effect of transgenic IL-37 expression on energy balance, including food intake and energy expenditure. Feeding homozygous IL-37 transgenic mice and wild-type (WT) control mice a high-fat diet (HFD; 45% kcal palm fat) for 6 weeks showed that IL-37 reduced body weight related to a marked decrease in food intake. Subsequent mechanistic studies in mice with heterozygous IL-37 expression versus WT littermates, fed the HFD for 18 weeks, confirmed that IL-37 reduces food intake, which led to a decrease in lean body mass, but did not reduce fat mass and plasma lipid levels or alterations in energy expenditure independent of lean body mass. Taken together, this suggests that IL-37 reduces lean body mass by reducing food intake.
topic IL-37
energy metabolism
food intake
high fat diet
url http://www.mdpi.com/1422-0067/19/8/2264
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