The fabrication of magnetic particle-based chemiluminescence immunoassay for human epididymis protein-4 detection in ovarian cancer

The fabrication of magnetic particle-based chemiluminescence immunoassay for human epididymis protein-4 detection in ovarian cancer

The magnetic particles have a significant influence on the immunoassay detection and cancer therapy. Herein, the chemiluminescence immunoassay combined with the magnetic particles (MPCLIA) was presented for the clinical determination and analysis of human epididymis protein 4 (HE4) in the human seru...

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Main Authors: Xiaoling Fu, Yangyang Liu, Ruiyun Qiu, Mohamed F. Foda, Yong Zhang, Tao Wang, Jinshan Li
Format: Article
Language:English
Published: Elsevier 2018-03-01
Series:Biochemistry and Biophysics Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2405580817302480
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spelling doaj-4dc5be391ec14600bf79c268dec671a92020-11-24T21:04:07ZengElsevierBiochemistry and Biophysics Reports2405-58082018-03-01137377The fabrication of magnetic particle-based chemiluminescence immunoassay for human epididymis protein-4 detection in ovarian cancerXiaoling Fu0Yangyang Liu1Ruiyun Qiu2Mohamed F. Foda3Yong Zhang4Tao Wang5Jinshan Li6Department of Orthopedic, The 2nd Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province 330006, PR ChinaCollege of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR ChinaComputer Center, Nanchang University, Nanchang, Jiangxi Province 330006, PR ChinaCollege of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China; State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China; Department of Biochemistry, Faculty of Agriculture, Benha University, Moshtohor, Toukh 13736, EgyptCollege of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR ChinaCollege of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR ChinaCollege of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China; State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China; Corresponding author at: College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.The magnetic particles have a significant influence on the immunoassay detection and cancer therapy. Herein, the chemiluminescence immunoassay combined with the magnetic particles (MPCLIA) was presented for the clinical determination and analysis of human epididymis protein 4 (HE4) in the human serum. Under the optimized experiment conditions, the secure MPCLIA method can detect HE4 in the broader range of 0–1000 pmol/L, with a lower detection limit of 1.35 pmol/L. The satisfactory recovery rate of the method in the serum ranged from 83.62% to 105.10%, which was well within the requirement of clinical analysis. Moreover, the results showed the good correlation with enzyme-linked immunosorbent assay (ELISA), with the correlation coefficient of 0.9589. This proposed method has been successfully applied to the clinical determination of HE4 in the human serum. Keywords: Chemiluminescence immunoassay, Magnetic particles, Human epididymis protein 4http://www.sciencedirect.com/science/article/pii/S2405580817302480
collection DOAJ
language English
format Article
sources DOAJ
author Xiaoling Fu
Yangyang Liu
Ruiyun Qiu
Mohamed F. Foda
Yong Zhang
Tao Wang
Jinshan Li
spellingShingle Xiaoling Fu
Yangyang Liu
Ruiyun Qiu
Mohamed F. Foda
Yong Zhang
Tao Wang
Jinshan Li
The fabrication of magnetic particle-based chemiluminescence immunoassay for human epididymis protein-4 detection in ovarian cancer
Biochemistry and Biophysics Reports
author_facet Xiaoling Fu
Yangyang Liu
Ruiyun Qiu
Mohamed F. Foda
Yong Zhang
Tao Wang
Jinshan Li
author_sort Xiaoling Fu
title The fabrication of magnetic particle-based chemiluminescence immunoassay for human epididymis protein-4 detection in ovarian cancer
title_short The fabrication of magnetic particle-based chemiluminescence immunoassay for human epididymis protein-4 detection in ovarian cancer
title_full The fabrication of magnetic particle-based chemiluminescence immunoassay for human epididymis protein-4 detection in ovarian cancer
title_fullStr The fabrication of magnetic particle-based chemiluminescence immunoassay for human epididymis protein-4 detection in ovarian cancer
title_full_unstemmed The fabrication of magnetic particle-based chemiluminescence immunoassay for human epididymis protein-4 detection in ovarian cancer
title_sort fabrication of magnetic particle-based chemiluminescence immunoassay for human epididymis protein-4 detection in ovarian cancer
publisher Elsevier
series Biochemistry and Biophysics Reports
issn 2405-5808
publishDate 2018-03-01
description The magnetic particles have a significant influence on the immunoassay detection and cancer therapy. Herein, the chemiluminescence immunoassay combined with the magnetic particles (MPCLIA) was presented for the clinical determination and analysis of human epididymis protein 4 (HE4) in the human serum. Under the optimized experiment conditions, the secure MPCLIA method can detect HE4 in the broader range of 0–1000 pmol/L, with a lower detection limit of 1.35 pmol/L. The satisfactory recovery rate of the method in the serum ranged from 83.62% to 105.10%, which was well within the requirement of clinical analysis. Moreover, the results showed the good correlation with enzyme-linked immunosorbent assay (ELISA), with the correlation coefficient of 0.9589. This proposed method has been successfully applied to the clinical determination of HE4 in the human serum. Keywords: Chemiluminescence immunoassay, Magnetic particles, Human epididymis protein 4
url http://www.sciencedirect.com/science/article/pii/S2405580817302480
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