Lumbrokinase effects on pro- and anti-apoptotic gene expression in Wistar rats with testicular torsion

• Main Authors: Danarto R, Heriyanto DS, Risan M, Yuri P
• Format: Article
• Language: English
• Published: Dove Medical Press 2019-09-01
• Series: Research and Reports in Urology
• Subjects: testicular torsion; pro-apoptotic genes; anti-apoptotic genes; lumbrokinase
• Online Access: https://www.dovepress.com/lumbrokinase-effects-on-pro--and-anti-apoptotic-gene-expression-in-wis-peer-reviewed-article-RRU

Raden Danarto,1 Didik Setyo Heriyanto,2 Muhammad Risan,1 Prahara Yuri1 1Division of Urology, Department of Surgery, Faculty of Medicine, Universitas Gadjah Mada/Dr. Sardjito Hospital, Yogyakarta 55281, Indonesia; 2Department of Anatomical Pathology, Faculty of Medicine, Universitas Gadjah Mada/Dr. Sardjito Hospital, Yogyakarta 55281, IndonesiaCorrespondence: Prahara YuriDivision of Urology, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito Hospital, Jl. Kesehatan No. 1, Yogyakarta 55281, IndonesiaEmail [e-mail]: Testicular reperfusion is believed to be the mechanism by which testicular injury occurs in the ischemic testis. This study was performed to determine the therapeutic efficacy of lumbrokinase for treating ischemia-reperfusion (IR) injury-induced bilateral testicular torsion.Methods: Twenty-four male rats were equally divided into the following groups: torsion only (T), torsion plus lumbrokinase (TL), torsion-detorsion only (TD) and torsion-detorsion plus lumbrokinase (TDL) groups. The right testicle in each groups sample was rotated 720° for 4 h, followed by orchiectomy. The rats in the TD (TD and TDL) groups additionally underwent detorsion for 1 h after the initial rotation. Testicular tissues were collected for measuring anti-apoptotic B-cell lymphoma-2 (BCL-2) and pro-apoptotic BCL-2-associated X protein (BAX) gene expression levels using real-time polymerase chain reaction.Results: Pro- and anti-apoptotic gene expression levels were increased in the TD groups. Lumbrokinase was significantly effective in lowering BAX expression levels, particularly those in the TDL group compared with those in the TD group (P<0.05). Lumbrokinase did not significant change BCL-2 expression levels.Conclusion: The administration of lumbrokinase before orchiectomy can protect against IR-induced testicular damage by reducing pro-apoptotic gene expression levels.Keywords: testicular torsion, pro-apoptotic genes, anti-apoptotic genes, lumbrokinase