YjbH regulates virulence genes expression and oxidative stress resistance in Staphylococcus aureus
We previously reported that disruption of the yjbI gene reduced virulence of Staphylococcus aureus. In this study, we found virulence in both silkworms and mice was restored by introducing the yjbH gene but not the yjbI gene to both yjbI and yjbH genes-disrupted mutants, suggesting that yjbH, the ge...
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Online Access: | http://dx.doi.org/10.1080/21505594.2021.1875683 |
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doaj-4e1bae3b7c0b45f58023bb8cf8dfba032021-01-26T12:33:40ZengTaylor & Francis GroupVirulence2150-55942150-56082021-01-0112147048010.1080/21505594.2021.18756831875683YjbH regulates virulence genes expression and oxidative stress resistance in Staphylococcus aureusAtmika Paudel0Suresh Panthee1Hiroshi Hamamoto2Tom Grunert3Kazuhisa Sekimizu4Teikyo University Institute of Medical MycologyTeikyo University Institute of Medical MycologyTeikyo University Institute of Medical MycologyUniversity of Veterinary MedicineTeikyo University Institute of Medical MycologyWe previously reported that disruption of the yjbI gene reduced virulence of Staphylococcus aureus. In this study, we found virulence in both silkworms and mice was restored by introducing the yjbH gene but not the yjbI gene to both yjbI and yjbH genes-disrupted mutants, suggesting that yjbH, the gene downstream to the yjbI gene in a two-gene operon-yjbIH, is responsible for this phenomenon. We further observed a decrease in various surface-associated proteins and changes in cell envelope glycostructures in the mutants. RNA-seq analysis revealed that disruption of the yjbI and the yjbH genes resulted in differential expression of a broad range of genes, notably, significant downregulation of genes involved in virulence and oxidative stress. Administration of N-acetyl-L-cysteine, a free-radical scavenger, restored the virulence in both the mutants. Our findings suggested that YjbH plays a role in staphylococcal pathogenicity by regulating virulence gene expression, affecting the bacterial surface structure, and conferring resistance to oxidative stress in a host.http://dx.doi.org/10.1080/21505594.2021.1875683staphylococcus aureusvirulenceyjbhyjbioxidative stresssurface structuregene expressionproteasepigmentsilkworm |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Atmika Paudel Suresh Panthee Hiroshi Hamamoto Tom Grunert Kazuhisa Sekimizu |
spellingShingle |
Atmika Paudel Suresh Panthee Hiroshi Hamamoto Tom Grunert Kazuhisa Sekimizu YjbH regulates virulence genes expression and oxidative stress resistance in Staphylococcus aureus Virulence staphylococcus aureus virulence yjbh yjbi oxidative stress surface structure gene expression protease pigment silkworm |
author_facet |
Atmika Paudel Suresh Panthee Hiroshi Hamamoto Tom Grunert Kazuhisa Sekimizu |
author_sort |
Atmika Paudel |
title |
YjbH regulates virulence genes expression and oxidative stress resistance in Staphylococcus aureus |
title_short |
YjbH regulates virulence genes expression and oxidative stress resistance in Staphylococcus aureus |
title_full |
YjbH regulates virulence genes expression and oxidative stress resistance in Staphylococcus aureus |
title_fullStr |
YjbH regulates virulence genes expression and oxidative stress resistance in Staphylococcus aureus |
title_full_unstemmed |
YjbH regulates virulence genes expression and oxidative stress resistance in Staphylococcus aureus |
title_sort |
yjbh regulates virulence genes expression and oxidative stress resistance in staphylococcus aureus |
publisher |
Taylor & Francis Group |
series |
Virulence |
issn |
2150-5594 2150-5608 |
publishDate |
2021-01-01 |
description |
We previously reported that disruption of the yjbI gene reduced virulence of Staphylococcus aureus. In this study, we found virulence in both silkworms and mice was restored by introducing the yjbH gene but not the yjbI gene to both yjbI and yjbH genes-disrupted mutants, suggesting that yjbH, the gene downstream to the yjbI gene in a two-gene operon-yjbIH, is responsible for this phenomenon. We further observed a decrease in various surface-associated proteins and changes in cell envelope glycostructures in the mutants. RNA-seq analysis revealed that disruption of the yjbI and the yjbH genes resulted in differential expression of a broad range of genes, notably, significant downregulation of genes involved in virulence and oxidative stress. Administration of N-acetyl-L-cysteine, a free-radical scavenger, restored the virulence in both the mutants. Our findings suggested that YjbH plays a role in staphylococcal pathogenicity by regulating virulence gene expression, affecting the bacterial surface structure, and conferring resistance to oxidative stress in a host. |
topic |
staphylococcus aureus virulence yjbh yjbi oxidative stress surface structure gene expression protease pigment silkworm |
url |
http://dx.doi.org/10.1080/21505594.2021.1875683 |
work_keys_str_mv |
AT atmikapaudel yjbhregulatesvirulencegenesexpressionandoxidativestressresistanceinstaphylococcusaureus AT sureshpanthee yjbhregulatesvirulencegenesexpressionandoxidativestressresistanceinstaphylococcusaureus AT hiroshihamamoto yjbhregulatesvirulencegenesexpressionandoxidativestressresistanceinstaphylococcusaureus AT tomgrunert yjbhregulatesvirulencegenesexpressionandoxidativestressresistanceinstaphylococcusaureus AT kazuhisasekimizu yjbhregulatesvirulencegenesexpressionandoxidativestressresistanceinstaphylococcusaureus |
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1724322585848578048 |