Site-Specific Expression Pattern of PIWI-Interacting RNA in Skin and Oral Mucosal Wound Healing
<b> </b>The<b> </b>oral mucosa exhibits exceptional healing capability when compared to skin. Recent studies suggest that intrinsic differences in coding genes and regulatory small non-coding RNA (sncRNA) genes (e.g., microRNAs) may underlie the exceptional healing that occur...
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doaj-4e324d58093c47acad6a8fd3e8dd83702020-11-25T00:35:16ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-01-0121252110.3390/ijms21020521ijms21020521Site-Specific Expression Pattern of PIWI-Interacting RNA in Skin and Oral Mucosal Wound HealingLin Chen0Zujian Chen1Alyne Simões2Xinming Wu3Yang Dai4Luisa A. DiPietro5Xiaofeng Zhou6Center for Wound Healing and Tissue Regeneration, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USADepartment of Periodontics, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USACenter for Wound Healing and Tissue Regeneration, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USADepartment of Periodontics, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USADepartment of Bioengineering, College of Engineering, University of Illinois at Chicago, Chicago, IL 60607, USACenter for Wound Healing and Tissue Regeneration, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USACenter for Wound Healing and Tissue Regeneration, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USA<b> </b>The<b> </b>oral mucosa exhibits exceptional healing capability when compared to skin. Recent studies suggest that intrinsic differences in coding genes and regulatory small non-coding RNA (sncRNA) genes (e.g., microRNAs) may underlie the exceptional healing that occurs in the oral mucosa. Here, we investigate the role of a novel class of sncRNA—Piwi-interacting RNA (piRNA)—in the tissue-specific differential response to injury. An abundance of piRNAs was detected in both skin and oral mucosal epithelium during wound healing. The expression of <i>PIWI</i> genes (the obligate binding partners of piRNAs) was also detected in skin and oral wound healing. This data suggested that PIWI-piRNA machinery may serve an unknown function in the highly orchestrated wound healing process. Furthermore, unique tissue-specific piRNA profiles were obtained in the skin and oral mucosal epithelium, and substantially more changes in piRNA expression were observed during skin wound healing than oral mucosal wound healing. Thus, we present the first clue suggesting a role of piRNA in wound healing, and provide the first site-specific piRNA profile of skin and oral mucosal wound healing. These results serve as a foundation for the future investigation of the functional contribution(s) of piRNA in wound repair and tissue regeneration.https://www.mdpi.com/1422-0067/21/2/521wound healingpirnapiwi geneoral mucosaskin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lin Chen Zujian Chen Alyne Simões Xinming Wu Yang Dai Luisa A. DiPietro Xiaofeng Zhou |
spellingShingle |
Lin Chen Zujian Chen Alyne Simões Xinming Wu Yang Dai Luisa A. DiPietro Xiaofeng Zhou Site-Specific Expression Pattern of PIWI-Interacting RNA in Skin and Oral Mucosal Wound Healing International Journal of Molecular Sciences wound healing pirna piwi gene oral mucosa skin |
author_facet |
Lin Chen Zujian Chen Alyne Simões Xinming Wu Yang Dai Luisa A. DiPietro Xiaofeng Zhou |
author_sort |
Lin Chen |
title |
Site-Specific Expression Pattern of PIWI-Interacting RNA in Skin and Oral Mucosal Wound Healing |
title_short |
Site-Specific Expression Pattern of PIWI-Interacting RNA in Skin and Oral Mucosal Wound Healing |
title_full |
Site-Specific Expression Pattern of PIWI-Interacting RNA in Skin and Oral Mucosal Wound Healing |
title_fullStr |
Site-Specific Expression Pattern of PIWI-Interacting RNA in Skin and Oral Mucosal Wound Healing |
title_full_unstemmed |
Site-Specific Expression Pattern of PIWI-Interacting RNA in Skin and Oral Mucosal Wound Healing |
title_sort |
site-specific expression pattern of piwi-interacting rna in skin and oral mucosal wound healing |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2020-01-01 |
description |
<b> </b>The<b> </b>oral mucosa exhibits exceptional healing capability when compared to skin. Recent studies suggest that intrinsic differences in coding genes and regulatory small non-coding RNA (sncRNA) genes (e.g., microRNAs) may underlie the exceptional healing that occurs in the oral mucosa. Here, we investigate the role of a novel class of sncRNA—Piwi-interacting RNA (piRNA)—in the tissue-specific differential response to injury. An abundance of piRNAs was detected in both skin and oral mucosal epithelium during wound healing. The expression of <i>PIWI</i> genes (the obligate binding partners of piRNAs) was also detected in skin and oral wound healing. This data suggested that PIWI-piRNA machinery may serve an unknown function in the highly orchestrated wound healing process. Furthermore, unique tissue-specific piRNA profiles were obtained in the skin and oral mucosal epithelium, and substantially more changes in piRNA expression were observed during skin wound healing than oral mucosal wound healing. Thus, we present the first clue suggesting a role of piRNA in wound healing, and provide the first site-specific piRNA profile of skin and oral mucosal wound healing. These results serve as a foundation for the future investigation of the functional contribution(s) of piRNA in wound repair and tissue regeneration. |
topic |
wound healing pirna piwi gene oral mucosa skin |
url |
https://www.mdpi.com/1422-0067/21/2/521 |
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