Novel fibrin-fibronectin matrix accelerates mice skin wound healing
Plasma fibrinogen (F1) and fibronectin (pFN) polymerize to form a fibrin clot that is both a hemostatic and provisional matrix for wound healing. About 90% of plasma F1 has a homodimeric pair of γ chains (γγF1), and 10% has a heterodimeric pair of γ and more acidic γ′ chains (γγ′F1). We have synthes...
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KeAi Communications Co., Ltd.
2020-12-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X20301183 |
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doaj-4e497cd22be0493a84c904fa1dd13ea92021-04-02T09:39:21ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2020-12-0154949962Novel fibrin-fibronectin matrix accelerates mice skin wound healingCarlos Poblete Jara0Ou Wang1Thais Paulino do Prado2Ayman Ismail3Frank Marco Fabian4Han Li5Licio A. Velloso6Mark A. Carlson7William Burgess8Yuguo Lei9William H. Velander10Eliana P. Araújo11Nursing School, University of Campinas, Campinas, SP, Brazil; Laboratory of Cell Signaling, University of Campinas, Campinas, SP, BrazilDepartment of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, NE, USA; Biomedical Engineering Program, University of Nebraska, Lincoln, NE, USANursing School, University of Campinas, Campinas, SP, Brazil; Laboratory of Cell Signaling, University of Campinas, Campinas, SP, BrazilDepartment of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, NE, USADepartment of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, NE, USADepartment of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, NE, USA; School of Biological Science, University of Nebraska, Lincoln, NE, USALaboratory of Cell Signaling, University of Campinas, Campinas, SP, BrazilDepartment of Surgery, University of Nebraska Medical Center and the VA Nebraska-Western Iowa Health Care System, USA; Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE, USADepartment of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, NE, USADepartment of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, NE, USA; Biomedical Engineering Program, University of Nebraska, Lincoln, NE, USA; Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE, USA; Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE, USA; Corresponding author. The University of Nebraska, Lincoln, NE, USA.Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, NE, USA; Corresponding author. The University of Nebraska, Lincoln, NE, USA.Nursing School, University of Campinas, Campinas, SP, Brazil; Laboratory of Cell Signaling, University of Campinas, Campinas, SP, Brazil; Corresponding author. The University of Campinas, Brazil.Plasma fibrinogen (F1) and fibronectin (pFN) polymerize to form a fibrin clot that is both a hemostatic and provisional matrix for wound healing. About 90% of plasma F1 has a homodimeric pair of γ chains (γγF1), and 10% has a heterodimeric pair of γ and more acidic γ′ chains (γγ′F1). We have synthesized a novel fibrin matrix exclusively from a 1:1 (molar ratio) complex of γγ′F1 and pFN in the presence of highly active thrombin and recombinant Factor XIII (rFXIIIa). In this matrix, the fibrin nanofibers were decorated with pFN nanoclusters (termed γγ′F1:pFN fibrin). In contrast, fibrin made from 1:1 mixture of γγF1 and pFN formed a sporadic distribution of “pFN droplets” (termed γγF1+pFN fibrin). The γγ′F1:pFN fibrin enhanced the adhesion of primary human umbilical vein endothelium cells (HUVECs) relative to the γγF1+FN fibrin. Three dimensional (3D) culturing showed that the γγ′F1:pFN complex fibrin matrix enhanced the proliferation of both HUVECs and primary human fibroblasts. HUVECs in the 3D γγ′F1:pFN fibrin exhibited a starkly enhanced vascular morphogenesis while an apoptotic growth profile was observed in the γγF1+pFN fibrin. Relative to γγF1+pFN fibrin, mouse dermal wounds that were sealed by γγ′F1:pFN fibrin exhibited accelerated and enhanced healing. This study suggests that a 3D pFN presentation on a fibrin matrix promotes wound healing.http://www.sciencedirect.com/science/article/pii/S2452199X20301183FibrinFibronectinNanoclustersWound healing |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Carlos Poblete Jara Ou Wang Thais Paulino do Prado Ayman Ismail Frank Marco Fabian Han Li Licio A. Velloso Mark A. Carlson William Burgess Yuguo Lei William H. Velander Eliana P. Araújo |
| spellingShingle |
Carlos Poblete Jara Ou Wang Thais Paulino do Prado Ayman Ismail Frank Marco Fabian Han Li Licio A. Velloso Mark A. Carlson William Burgess Yuguo Lei William H. Velander Eliana P. Araújo Novel fibrin-fibronectin matrix accelerates mice skin wound healing Bioactive Materials Fibrin Fibronectin Nanoclusters Wound healing |
| author_facet |
Carlos Poblete Jara Ou Wang Thais Paulino do Prado Ayman Ismail Frank Marco Fabian Han Li Licio A. Velloso Mark A. Carlson William Burgess Yuguo Lei William H. Velander Eliana P. Araújo |
| author_sort |
Carlos Poblete Jara |
| title |
Novel fibrin-fibronectin matrix accelerates mice skin wound healing |
| title_short |
Novel fibrin-fibronectin matrix accelerates mice skin wound healing |
| title_full |
Novel fibrin-fibronectin matrix accelerates mice skin wound healing |
| title_fullStr |
Novel fibrin-fibronectin matrix accelerates mice skin wound healing |
| title_full_unstemmed |
Novel fibrin-fibronectin matrix accelerates mice skin wound healing |
| title_sort |
novel fibrin-fibronectin matrix accelerates mice skin wound healing |
| publisher |
KeAi Communications Co., Ltd. |
| series |
Bioactive Materials |
| issn |
2452-199X |
| publishDate |
2020-12-01 |
| description |
Plasma fibrinogen (F1) and fibronectin (pFN) polymerize to form a fibrin clot that is both a hemostatic and provisional matrix for wound healing. About 90% of plasma F1 has a homodimeric pair of γ chains (γγF1), and 10% has a heterodimeric pair of γ and more acidic γ′ chains (γγ′F1). We have synthesized a novel fibrin matrix exclusively from a 1:1 (molar ratio) complex of γγ′F1 and pFN in the presence of highly active thrombin and recombinant Factor XIII (rFXIIIa). In this matrix, the fibrin nanofibers were decorated with pFN nanoclusters (termed γγ′F1:pFN fibrin). In contrast, fibrin made from 1:1 mixture of γγF1 and pFN formed a sporadic distribution of “pFN droplets” (termed γγF1+pFN fibrin). The γγ′F1:pFN fibrin enhanced the adhesion of primary human umbilical vein endothelium cells (HUVECs) relative to the γγF1+FN fibrin. Three dimensional (3D) culturing showed that the γγ′F1:pFN complex fibrin matrix enhanced the proliferation of both HUVECs and primary human fibroblasts. HUVECs in the 3D γγ′F1:pFN fibrin exhibited a starkly enhanced vascular morphogenesis while an apoptotic growth profile was observed in the γγF1+pFN fibrin. Relative to γγF1+pFN fibrin, mouse dermal wounds that were sealed by γγ′F1:pFN fibrin exhibited accelerated and enhanced healing. This study suggests that a 3D pFN presentation on a fibrin matrix promotes wound healing. |
| topic |
Fibrin Fibronectin Nanoclusters Wound healing |
| url |
http://www.sciencedirect.com/science/article/pii/S2452199X20301183 |
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