Biofilm Formation in Staphylococcus Aureus and its Relation to Phenotypic and Genotypic Criteria

Abstract Background and Objective: Biofilm is a complex microbial community embedded in a self-produced extracellular polymeric matrix. We aimed to study the extent of biofilm formation by S. Areas isolates and its relation to some phenotypic and genotypic criteria. Material and Methods: One hundre...

Full description

Bibliographic Details
Main Authors: M Hasannejad Bibalan, N Javid, M Samet, F Shakeri, EA Ghaemi
Format: Article
Language:English
Published: Golestan University of Medical Sciences 2014-08-01
Series:Medical Laboratory Journal
Subjects:
pcr
Online Access:http://mlj.goums.ac.ir/article-1-504-en.html
id doaj-4e8e59ab332e4f398cf9eed912e06922
record_format Article
spelling doaj-4e8e59ab332e4f398cf9eed912e069222021-09-01T13:20:25ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492014-08-018317Biofilm Formation in Staphylococcus Aureus and its Relation to Phenotypic and Genotypic CriteriaM Hasannejad Bibalan0N Javid1M Samet2F Shakeri3EA Ghaemi4 Abstract Background and Objective: Biofilm is a complex microbial community embedded in a self-produced extracellular polymeric matrix. We aimed to study the extent of biofilm formation by S. Areas isolates and its relation to some phenotypic and genotypic criteria. Material and Methods: One hundred-fifty strains of Staphylococcus aureus isolated from Gorgan were studied. Microtiter plate assay method was used for investigation of biofilm formation.The biofilm formation of strains were recorded and its relation to accessory gene regulator (agr) and antibiotic resistance were assessed by X2 test. Results: Eighty-four isolates (56%) were able to form biofilm. The strength of biofilm formation in agr group I was more than that of other groups. The biofilm formation among S. Areas isolated from the wound and urine (both with 75 %) had the highest capability. Methicillin-resistant isolates had a greater ability to biofilm formation. Conclusion: Methicillin resistant isolates had a greater ability to biofilm formation. Given the importance and treatment related problems of Methicillin-Resistant Staphylococcus Aureus (MRSA) especially Community Acquired-Methicillin-Resistant Staphylococcus Aureus (CA-MRSA), it is a necessity to control or remove the biofilm formation alongside antibiotic treatment. Keywords: Staphylococcus Aureus, Biofilm, Microtiter Plates Assay, PCRhttp://mlj.goums.ac.ir/article-1-504-en.htmlstaphylococcus aureusbiofilmmicrotiter plates assaypcr
collection DOAJ
language English
format Article
sources DOAJ
author M Hasannejad Bibalan
N Javid
M Samet
F Shakeri
EA Ghaemi
spellingShingle M Hasannejad Bibalan
N Javid
M Samet
F Shakeri
EA Ghaemi
Biofilm Formation in Staphylococcus Aureus and its Relation to Phenotypic and Genotypic Criteria
Medical Laboratory Journal
staphylococcus aureus
biofilm
microtiter plates assay
pcr
author_facet M Hasannejad Bibalan
N Javid
M Samet
F Shakeri
EA Ghaemi
author_sort M Hasannejad Bibalan
title Biofilm Formation in Staphylococcus Aureus and its Relation to Phenotypic and Genotypic Criteria
title_short Biofilm Formation in Staphylococcus Aureus and its Relation to Phenotypic and Genotypic Criteria
title_full Biofilm Formation in Staphylococcus Aureus and its Relation to Phenotypic and Genotypic Criteria
title_fullStr Biofilm Formation in Staphylococcus Aureus and its Relation to Phenotypic and Genotypic Criteria
title_full_unstemmed Biofilm Formation in Staphylococcus Aureus and its Relation to Phenotypic and Genotypic Criteria
title_sort biofilm formation in staphylococcus aureus and its relation to phenotypic and genotypic criteria
publisher Golestan University of Medical Sciences
series Medical Laboratory Journal
issn 2538-4449
publishDate 2014-08-01
description Abstract Background and Objective: Biofilm is a complex microbial community embedded in a self-produced extracellular polymeric matrix. We aimed to study the extent of biofilm formation by S. Areas isolates and its relation to some phenotypic and genotypic criteria. Material and Methods: One hundred-fifty strains of Staphylococcus aureus isolated from Gorgan were studied. Microtiter plate assay method was used for investigation of biofilm formation.The biofilm formation of strains were recorded and its relation to accessory gene regulator (agr) and antibiotic resistance were assessed by X2 test. Results: Eighty-four isolates (56%) were able to form biofilm. The strength of biofilm formation in agr group I was more than that of other groups. The biofilm formation among S. Areas isolated from the wound and urine (both with 75 %) had the highest capability. Methicillin-resistant isolates had a greater ability to biofilm formation. Conclusion: Methicillin resistant isolates had a greater ability to biofilm formation. Given the importance and treatment related problems of Methicillin-Resistant Staphylococcus Aureus (MRSA) especially Community Acquired-Methicillin-Resistant Staphylococcus Aureus (CA-MRSA), it is a necessity to control or remove the biofilm formation alongside antibiotic treatment. Keywords: Staphylococcus Aureus, Biofilm, Microtiter Plates Assay, PCR
topic staphylococcus aureus
biofilm
microtiter plates assay
pcr
url http://mlj.goums.ac.ir/article-1-504-en.html
work_keys_str_mv AT mhasannejadbibalan biofilmformationinstaphylococcusaureusanditsrelationtophenotypicandgenotypiccriteria
AT njavid biofilmformationinstaphylococcusaureusanditsrelationtophenotypicandgenotypiccriteria
AT msamet biofilmformationinstaphylococcusaureusanditsrelationtophenotypicandgenotypiccriteria
AT fshakeri biofilmformationinstaphylococcusaureusanditsrelationtophenotypicandgenotypiccriteria
AT eaghaemi biofilmformationinstaphylococcusaureusanditsrelationtophenotypicandgenotypiccriteria
_version_ 1721182652650749952