Biofilm Formation in Staphylococcus Aureus and its Relation to Phenotypic and Genotypic Criteria
Abstract Background and Objective: Biofilm is a complex microbial community embedded in a self-produced extracellular polymeric matrix. We aimed to study the extent of biofilm formation by S. Areas isolates and its relation to some phenotypic and genotypic criteria. Material and Methods: One hundre...
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doaj-4e8e59ab332e4f398cf9eed912e069222021-09-01T13:20:25ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492014-08-018317Biofilm Formation in Staphylococcus Aureus and its Relation to Phenotypic and Genotypic CriteriaM Hasannejad Bibalan0N Javid1M Samet2F Shakeri3EA Ghaemi4 Abstract Background and Objective: Biofilm is a complex microbial community embedded in a self-produced extracellular polymeric matrix. We aimed to study the extent of biofilm formation by S. Areas isolates and its relation to some phenotypic and genotypic criteria. Material and Methods: One hundred-fifty strains of Staphylococcus aureus isolated from Gorgan were studied. Microtiter plate assay method was used for investigation of biofilm formation.The biofilm formation of strains were recorded and its relation to accessory gene regulator (agr) and antibiotic resistance were assessed by X2 test. Results: Eighty-four isolates (56%) were able to form biofilm. The strength of biofilm formation in agr group I was more than that of other groups. The biofilm formation among S. Areas isolated from the wound and urine (both with 75 %) had the highest capability. Methicillin-resistant isolates had a greater ability to biofilm formation. Conclusion: Methicillin resistant isolates had a greater ability to biofilm formation. Given the importance and treatment related problems of Methicillin-Resistant Staphylococcus Aureus (MRSA) especially Community Acquired-Methicillin-Resistant Staphylococcus Aureus (CA-MRSA), it is a necessity to control or remove the biofilm formation alongside antibiotic treatment. Keywords: Staphylococcus Aureus, Biofilm, Microtiter Plates Assay, PCRhttp://mlj.goums.ac.ir/article-1-504-en.htmlstaphylococcus aureusbiofilmmicrotiter plates assaypcr |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
M Hasannejad Bibalan N Javid M Samet F Shakeri EA Ghaemi |
spellingShingle |
M Hasannejad Bibalan N Javid M Samet F Shakeri EA Ghaemi Biofilm Formation in Staphylococcus Aureus and its Relation to Phenotypic and Genotypic Criteria Medical Laboratory Journal staphylococcus aureus biofilm microtiter plates assay pcr |
author_facet |
M Hasannejad Bibalan N Javid M Samet F Shakeri EA Ghaemi |
author_sort |
M Hasannejad Bibalan |
title |
Biofilm Formation in Staphylococcus Aureus and its Relation to Phenotypic and Genotypic Criteria |
title_short |
Biofilm Formation in Staphylococcus Aureus and its Relation to Phenotypic and Genotypic Criteria |
title_full |
Biofilm Formation in Staphylococcus Aureus and its Relation to Phenotypic and Genotypic Criteria |
title_fullStr |
Biofilm Formation in Staphylococcus Aureus and its Relation to Phenotypic and Genotypic Criteria |
title_full_unstemmed |
Biofilm Formation in Staphylococcus Aureus and its Relation to Phenotypic and Genotypic Criteria |
title_sort |
biofilm formation in staphylococcus aureus and its relation to phenotypic and genotypic criteria |
publisher |
Golestan University of Medical Sciences |
series |
Medical Laboratory Journal |
issn |
2538-4449 |
publishDate |
2014-08-01 |
description |
Abstract
Background and Objective: Biofilm is a complex microbial community embedded in a self-produced extracellular polymeric matrix. We aimed to study the extent of biofilm formation by S. Areas isolates and its relation to some phenotypic and genotypic criteria.
Material and Methods: One hundred-fifty strains of Staphylococcus aureus isolated from Gorgan were studied. Microtiter plate assay method was used for investigation of biofilm formation.The biofilm formation of strains were recorded and its relation to accessory gene regulator (agr) and antibiotic resistance were assessed by X2 test.
Results: Eighty-four isolates (56%) were able to form biofilm. The strength of biofilm formation in agr group I was more than that of other groups. The biofilm formation among S. Areas isolated from the wound and urine (both with 75 %) had the highest capability. Methicillin-resistant isolates had a greater ability to biofilm formation.
Conclusion: Methicillin resistant isolates had a greater ability to biofilm formation. Given the importance and treatment related problems of Methicillin-Resistant Staphylococcus Aureus (MRSA) especially Community Acquired-Methicillin-Resistant Staphylococcus Aureus (CA-MRSA), it is a necessity to control or remove the biofilm formation alongside antibiotic treatment.
Keywords: Staphylococcus Aureus, Biofilm, Microtiter Plates Assay, PCR |
topic |
staphylococcus aureus biofilm microtiter plates assay pcr |
url |
http://mlj.goums.ac.ir/article-1-504-en.html |
work_keys_str_mv |
AT mhasannejadbibalan biofilmformationinstaphylococcusaureusanditsrelationtophenotypicandgenotypiccriteria AT njavid biofilmformationinstaphylococcusaureusanditsrelationtophenotypicandgenotypiccriteria AT msamet biofilmformationinstaphylococcusaureusanditsrelationtophenotypicandgenotypiccriteria AT fshakeri biofilmformationinstaphylococcusaureusanditsrelationtophenotypicandgenotypiccriteria AT eaghaemi biofilmformationinstaphylococcusaureusanditsrelationtophenotypicandgenotypiccriteria |
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